Catalog No.
RHF46902
Species reactivity
Human
Host species
Mouse
Isotype
IgG2b
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
Ubiquitin-like protein SMT3B, SMT3B, SUMO-3, Smt3B, Sentrin-2, SMT3 homolog 2, SMT3H2, SUMO2, HSMT3, Small ubiquitin-related modifier 2, SUMO-2, SUMO-3, Ubiquitin-like protein SMT3A, SMT3A, SUMO3, SMT3 homolog 1, SMT3H1, Small ubiquitin-related modifier 3, SUMO-2, Smt3A
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P61956, P55854
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3N97
SUMO2 inhibits ACSL3 protein degradation to antagonize erastin-induced ferroptosis in hepatocellular carcinoma., PMID:40526170
A highly sensitive nanochannel device for the detection of SUMO1 peptides., PMID:37564410
An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathway., PMID:36860866
SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate., PMID:36494414
Proteomics datasets of developing rat brain: Synaptic proteome and SUMO2/3-ylome., PMID:35516005
Exploration of autoantibody responses in canine diabetes using protein arrays., PMID:35169238
Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain., PMID:34887727
SUMO-Modification of Human Nrf2 at K110 and K533 Regulates Its Nucleocytoplasmic Localization, Stability and Transcriptional Activity., PMID:33770425
Antibody-free enrichment method for proteome-wide analysis of endogenous SUMOylation sites., PMID:33736815
Proteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation., PMID:33472076
SUMOylation Is Required for ERK5 Nuclear Translocation and ERK5-Mediated Cancer Cell Proliferation., PMID:32209980
Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology., PMID:29138295
SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells., PMID:29038616
Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins., PMID:27631801
Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions Between SUMO Traps and Mono- or PolySUMO Moieties., PMID:27631800
IL-6 induced proliferation and cytotoxic activity of CD8(+) T cells is elevated by SUMO2 overexpression., PMID:27071615
Lung neuroendocrine tumors: correlation of ubiquitinylation and sumoylation with nucleo-cytosolic partitioning of PTEN., PMID:25884169
The SUMO-targeted ubiquitin ligase RNF4 localizes to etoposide-exposed mitotic chromosomes: implication for a novel DNA damage response during mitosis., PMID:24695317
Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies., PMID:24651501
In vivo characterization of the properties of SUMO1-specific monobodies., PMID:24040933
Detecting endogenous SUMO targets in mammalian cells and tissues., PMID:23503365
SUMOylation of the inducible (c-Fos:c-Jun)/AP-1 transcription complex occurs on target promoters to limit transcriptional activation., PMID:23396363
Identification of endogenous SUMO1 accepter sites by mass spectrometry., PMID:22665316
Ubiquitin-related proteins in neuronal and glial intranuclear inclusions in intranuclear inclusion body disease., PMID:22612509
Small ubiquitin-like modifier (SUMO) modification mediates function of the inhibitory domains of developmental regulators FOXC1 and FOXC2., PMID:22493429
Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation., PMID:22118216
Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5., PMID:21931855
Evidence Implicating CCNB1IP1, a RING Domain-Containing Protein Required for Meiotic Crossing Over in Mice, as an E3 SUMO Ligase., PMID:21779533
SUMO proteins are involved in the stress response during spermatogenesis and are localized to DNA double-strand breaks in germ cells., PMID:20385780
Nuclear proteome analysis of monkey embryonic stem cells during differentiation., PMID:20091144
"ChopNSpice," a mass spectrometric approach that allows identification of endogenous small ubiquitin-like modifier-conjugated peptides., PMID:19721078
[Prokaryotic expression, purification and preparation of polyclonal antibody for human SUMO-2 gene]., PMID:18616917
Stabilization of PML nuclear localization by conjugation and oligomerization of SUMO-3., PMID:15940266
Small ubiquitin-related modifiers: A novel and independent class of autoantigens in primary biliary cirrhosis., PMID:15726652
Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1., PMID:12665592
A novel mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localized in the nucleolus at interphase., PMID:11029585
SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage., PMID:10759568
Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3., PMID:10692421