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Anti-SUMO2/3 Antibody (R3N97)

Catalog #:   RHF46902 Specific References (38) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: WB
Accession: P61956, P55854
Overview

Catalog No.

RHF46902

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

WB: 1:500-1:1000

Target

Ubiquitin-like protein SMT3B, SMT3B, SUMO-3, Smt3B, Sentrin-2, SMT3 homolog 2, SMT3H2, SUMO2, HSMT3, Small ubiquitin-related modifier 2, SUMO-2, SUMO-3, Ubiquitin-like protein SMT3A, SMT3A, SUMO3, SMT3 homolog 1, SMT3H1, Small ubiquitin-related modifier 3, SUMO-2, Smt3A

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P61956, P55854

Applications

WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N97

Data Image
  • Western blot
    Western blot analysis of SUMO2/3 in Hela lysates using SUMO2/3 antibody.
References

SUMO2 inhibits ACSL3 protein degradation to antagonize erastin-induced ferroptosis in hepatocellular carcinoma., PMID:40526170

A highly sensitive nanochannel device for the detection of SUMO1 peptides., PMID:37564410

An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathway., PMID:36860866

SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate., PMID:36494414

Proteomics datasets of developing rat brain: Synaptic proteome and SUMO2/3-ylome., PMID:35516005

Exploration of autoantibody responses in canine diabetes using protein arrays., PMID:35169238

Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain., PMID:34887727

SUMO-Modification of Human Nrf2 at K110 and K533 Regulates Its Nucleocytoplasmic Localization, Stability and Transcriptional Activity., PMID:33770425

Antibody-free enrichment method for proteome-wide analysis of endogenous SUMOylation sites., PMID:33736815

Proteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation., PMID:33472076

SUMOylation Is Required for ERK5 Nuclear Translocation and ERK5-Mediated Cancer Cell Proliferation., PMID:32209980

Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology., PMID:29138295

SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells., PMID:29038616

Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins., PMID:27631801

Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions Between SUMO Traps and Mono- or PolySUMO Moieties., PMID:27631800

IL-6 induced proliferation and cytotoxic activity of CD8(+) T cells is elevated by SUMO2 overexpression., PMID:27071615

Lung neuroendocrine tumors: correlation of ubiquitinylation and sumoylation with nucleo-cytosolic partitioning of PTEN., PMID:25884169

The SUMO-targeted ubiquitin ligase RNF4 localizes to etoposide-exposed mitotic chromosomes: implication for a novel DNA damage response during mitosis., PMID:24695317

Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies., PMID:24651501

In vivo characterization of the properties of SUMO1-specific monobodies., PMID:24040933

Detecting endogenous SUMO targets in mammalian cells and tissues., PMID:23503365

SUMOylation of the inducible (c-Fos:c-Jun)/AP-1 transcription complex occurs on target promoters to limit transcriptional activation., PMID:23396363

Identification of endogenous SUMO1 accepter sites by mass spectrometry., PMID:22665316

Ubiquitin-related proteins in neuronal and glial intranuclear inclusions in intranuclear inclusion body disease., PMID:22612509

Small ubiquitin-like modifier (SUMO) modification mediates function of the inhibitory domains of developmental regulators FOXC1 and FOXC2., PMID:22493429

Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation., PMID:22118216

Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5., PMID:21931855

Evidence Implicating CCNB1IP1, a RING Domain-Containing Protein Required for Meiotic Crossing Over in Mice, as an E3 SUMO Ligase., PMID:21779533

SUMO proteins are involved in the stress response during spermatogenesis and are localized to DNA double-strand breaks in germ cells., PMID:20385780

Nuclear proteome analysis of monkey embryonic stem cells during differentiation., PMID:20091144

"ChopNSpice," a mass spectrometric approach that allows identification of endogenous small ubiquitin-like modifier-conjugated peptides., PMID:19721078

[Prokaryotic expression, purification and preparation of polyclonal antibody for human SUMO-2 gene]., PMID:18616917

Stabilization of PML nuclear localization by conjugation and oligomerization of SUMO-3., PMID:15940266

Small ubiquitin-related modifiers: A novel and independent class of autoantigens in primary biliary cirrhosis., PMID:15726652

Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1., PMID:12665592

A novel mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localized in the nucleolus at interphase., PMID:11029585

SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage., PMID:10759568

Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3., PMID:10692421

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-SUMO2/3 Antibody (R3N97) [RHF46902]
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