Overview
Catalog No.
RHF45201
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000
Target
Rnd3, RHO8, RND3, Rho-related GTP-binding protein RhoE, Protein MemB, Rho family GTPase 3, Rho-related GTP-binding protein Rho8, ARHE, RHOE
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P61587
Applications
ELISA, FCM, IF, IHC
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3N95
Data Image
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Flow cytometric analysis of Hela cells using RND3 mouse mAb (green) and negative control (red).
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Immunofluorescence analysis of Hela cells using RND3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using RND3 mouse mAb with DAB staining.
References
Mechanistic role of RND3-regulated IL33/ST2 signaling on cardiomyocyte senescence., PMID:38724005
Identification and characterization of a new isoform of small GTPase RhoE., PMID:33060740
Chaperone-mediated autophagy regulates proliferation by targeting RND3 in gastric cancer., PMID:26761524
Melanoma-expressed CD70 is involved in invasion and metastasis., PMID:26671750
Neuronal polarization is impaired in mice lacking RhoE expression., PMID:22428561
p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival., PMID:21079653
Expression of RND proteins in human myometrium., PMID:16554414
RhoE function is regulated by ROCK I-mediated phosphorylation., PMID:15775972
Datasheet
For research use only. Not for human or drug use.
