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Anti-RHOA Antibody (R3N93)

Catalog #:   RHF45102 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, FCM, IF, IHC, WB
Accession: P61586
Overview

Catalog No.

RHF45102

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Rho cDNA clone 12, ARH12, RHO12, ARHA, RHOA, Transforming protein RhoA, h12

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P61586

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N93

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using RHOA mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of HepG2 cells using RHOA mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using RHOA mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of K562 cells using RHOA mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using RHOA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using RHOA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using RHOA mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissues using RHOA mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using RHOA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.
References

Comparison of the Mutational Profile between BCL2- and BCL6-Rearrangement Positive Follicular Lymphoma., PMID:40482882

Epithelial eversion, a collective rearrangement from apical-in to apical-out polarity, is initiated by α6β4 integrins and sustained by increased cell proliferation and anchorage-independence., PMID:40463241

Biological and Clinical Relevance of Genetic Alterations in Peripheral T-cell Lymphomas., PMID:40416001

PRRG4 Brain-Specific Conditional Knockout Mice Display Autism Spectrum Disorder-Like Behaviors., PMID:40380139

Orphan GPR50 Restrains Neurite Outgrowth and Cell Migration by Activating the G12/13 Protein-RhoA Pathway in Neural Progenitor Cells and Tanycytes., PMID:40091563

Binding of RHOA G17V to p300 enhances its HAT activity: a new mechanism of epigenetic deregulation in TFH lymphoma., PMID:40020168

The potential role of RhoA/ROCK-inhibition on locomotor recovery after spinal cord injury: a systematic review of in-vivo studies., PMID:39956860

In Vitro Experiment Present ROCK2 Inhibition Promotes the Therapeutic Effect of Bevacizumab in the Treatment of Glioblastoma Multiforme., PMID:39792535

Tetraspanin CD81 serves as a functional entry factor for porcine circovirus type 2 infection., PMID:39745447

Anillin interacts with RhoA to promote tumor progression in anaplastic thyroid cancer by activating the PI3K/AKT pathway., PMID:39738892

Regulation of BCR-dependent germinal center B-cell formation by HGAL and insight into its emerging myeloid ortholog, C1ORF150., PMID:39474423

Focal Adhesion Kinase (FAK) inhibition induces membrane accumulation of aquaporin2 (AQP2) through endocytosis inhibition and actin depolymerization in renal epithelial cells., PMID:39416213

IL-8 promotes lens capsular residual cells migration by down-regulates expression of E-cadherin and ZO-1 via the CXCR1/2-NF-κB-RhoA signal pathway., PMID:39244903

CD64+ fibroblast-targeted vilanterol and a STING agonist augment CLDN18.2 BiTEs efficacy against pancreatic cancer by reducing desmoplasia and enriching stem-like CD8+ T cells., PMID:39187291

IL-13 and IL-17A activate β1 integrin through an NF-kB/Rho kinase/PIP5K1γ pathway to enhance force transmission in airway smooth muscle., PMID:39136993

A combination of 5-azacytidine and nivolumab is a potentially effective rescue therapy in relapsed/refractory AITL., PMID:38983865

Tumor necrosis factor α receptor 1A transduces the inhibitory effect on axon regeneration triggered by IgG anti-ganglioside GD1a antibodies., PMID:38897255

Desmosomal Cadherin Tension Loss in Pemphigus Vulgaris Mediated by the Inhibition of Active RhoA at Cell-Cell Adhesions., PMID:38766211

IL-13 and IL-17A Activate β1 Integrin through an NF-kB/Rho kinase/PIP5K1γ pathway to Enhance Force Transmission in Airway Smooth Muscle., PMID:38746410

The ion channel TRPV5 regulates B-cell signaling and activation., PMID:38694510

Mutational, immune microenvironment, and clinicopathological profiles of diffuse large B-cell lymphoma and follicular lymphoma with BCL6 rearrangement., PMID:38462571

Mesenchymal Transglutaminase 2 Activates Epithelial ADAM17: Link to G-Protein-Coupled Receptor 56 (ADGRG1) Signalling., PMID:38397010

PLK1 phosphorylates RhoGDI1 and promotes cancer cell migration and invasion., PMID:38355643

Geranylgeranyl isoprenoids and hepatic Rap1a regulate basal and statin-induced expression of PCSK9., PMID:38309417

Suppression of Mast Cell Activation by GPR35: GPR35 Is a Primary Target of Disodium Cromoglycate., PMID:38290974

Angiogenic and Fibrogenic Dual-effect of Gremlin1 on Proliferative Diabetic Retinopathy., PMID:38250154

Fluorescent peptide for detecting factor XIIIa activity and fibrin in whole blood clots forming under flow., PMID:38222077

Placenta microRNA profile of patient with Obstetric Antiphospholipid Syndrome., PMID:38155384

Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function., PMID:38049580

Blockade of Rho-associated kinase prevents inhibition of axon regeneration of peripheral nerves induced by anti-ganglioside antibodies., PMID:37843226

RhoA vesicle trafficking-mediated transglutaminase 2 membrane translocation promotes IgA1 mesangial deposition in IgA nephropathy., PMID:37811653

Extracellular pyruvate kinase M2 induces cell migration through p-Tyr42 RhoA-mediated superoxide generation and epithelial-mesenchymal transition., PMID:37722568

RhoA-LIMK Signaling Axis Reveals Rostral-Caudal Plane and Spatial Dysregulation in the Brain of Alzheimer's Disease Mouse Models., PMID:37718806

Composite B-cell and T-cell lymphomas: clinical, pathological, and molecular features of three cases and literature review., PMID:37551557

IGF2BP3/HIF1A/YAP signaling plays a role in driving acute-on-chronic liver failure through activating hepatocyte reprogramming., PMID:37257765

Antibodies Directed Against GalNAc- and GlcNAc-O-Tyrosine Posttranslational Modifications - a New Tool for Glycoproteomic Detection., PMID:37052513

Vascular mechanisms of post-COVID-19 conditions: Rho-kinase is a novel target for therapy., PMID:37019821

Pathologic and molecular insights in nodal T-follicular helper cell lymphomas., PMID:36793612

Endothelial-Specific Targeting of RhoA Signaling via CD31 Antibody-Conjugated Nanoparticles., PMID:36746610

ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cells., PMID:36631535

Flow Cytometry Analysis Reveals a Wide Cytologic and Immunophenotypic Spectrum of Peripheral B Lymphocytes in Angioimmunoblastic T-Cell Lymphoma., PMID:36423589

Involvement of Paired Immunoglobulin-Like Receptor B in Cognitive Dysfunction Through Hippocampal-Dependent Synaptic Plasticity Impairments in Mice Subjected to Chronic Sleep Restriction., PMID:36417104

CD16+ fibroblasts foster a trastuzumab-refractory microenvironment that is reversed by VAV2 inhibition., PMID:36379207

Microtubules restrict F-actin polymerization to the immune synapse via GEF-H1 to maintain polarity in lymphocytes., PMID:36111670

Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signaling., PMID:36059552

Clonal germinal center B cells function as a niche for T-cell lymphoma., PMID:35921527

Stool Interleukin-1β Differentiates Clostridioides difficile Infection (CDI) From Asymptomatic Carriage and Non-CDI Diarrhea., PMID:35906836

Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells., PMID:35883755

Autocrine pro-legumain promotes breast cancer metastasis via binding to integrin αvβ3., PMID:35854065

The Antibody Dependant Neurite Outgrowth Modulation Response Involvement in Spinal Cord Injury., PMID:35784350

Datasheet

Document Download

Anti-RHOA Antibody (R3N93).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-RHOA Antibody (R3N93) [RHF45102]
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