Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Recombinant Antibody

Anti-ACTB/β-actin/Beta Actin Antibody (R3N80)

Catalog #:   RHF37503 Specific References (32) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: FCM, IF, IP, WB
Accession: P60709
Overview

Catalog No.

RHF37503

Species reactivity

Human, Mouse, Rat, Dog, Hamster, Monkey

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:50-1:500, IP: 1:100-1:200, WB: 1:10000-1:40000

Target

Beta-actin, ACTB, Actin, cytoplasmic 1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P60709

Applications

FCM, IF, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N80

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).
  • Immunofluorescence
    Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
  • Immunoprecipitation
    Immunoprecipitation using beta-Actin mouse mAb(dilution: 1/250) against HL-60,C2C12,NRK,RSC,Hela,Cos7 cell lysate.
  • Western blot
    Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
  • Western blot
    Western blot analysis using beta-Actin mouse mAb against Rat serum(1),Rabbit serum(2),PC-12(3),Raw264.7(4),NIH/3T3(5),NRK(6),C2C12(7),C6(8),L1210(9),F9(10),CHO3D10(11),Hela(12),Jurkat(13) cell lysate.
  • Western blot
    Western blot analysis using beta-Actin mouse mAb against Rat serum(1),Rabbit serum(2),PC-12(3),Raw264.7(4),NIH/3T3(5),NRK(6),C2C12(7),C6(8),L1210(9),F9(10),CHO3D10(11),Hela(12),Jurkat(13) cell lysate.
References

Si Microanemones Integrated Microfluidic Chip for Highly Efficient Isolation of Extracellular Vesicles., PMID:40395100

Exploring heterogeneous expression of beta-actin (ACTB) in bladder cancer by producing a monoclonal antibody 6D6., PMID:38867273

Proteomic Analysis of Egg Yolk Proteins During Embryonic Development in Wanxi White Goose., PMID:38433387

Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair., PMID:37978868

Alterations in the expression pattern of RBC membrane associated proteins (RMAPs) in whole body γ-irradiated Sprague Dawley rats., PMID:37315317

Porcine reproductive and respiratory syndrome virus degrades DDX10 via SQSTM1/p62-dependent selective autophagy to antagonize its antiviral activity., PMID:36779599

Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells., PMID:35899934

Low anti-CFL1 antibody with high anti-ACTB antibody is a poor prognostic factor in esophageal squamous cell carcinoma., PMID:35780443

Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis., PMID:35491858

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD., PMID:35473441

Routine noninvasive prenatal screening for fetal Rh D in maternal plasma-A 2-year experience from a single center in Belgium., PMID:35352833

Circulating Mir-140 and leptin improve the accuracy of the differential diagnosis between psoriatic arthritis and rheumatoid arthritis: a case-control study., PMID:34380068

Novel serum autoantibodies against ß-actin (ACTB) in amyotrophic lateral sclerosis., PMID:33792444

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

TFEB rearranged renal cell carcinoma. A clinicopathologic and molecular study of 13 cases. Tumors harboring MALAT1-TFEB, ACTB-TFEB, and the novel NEAT1-TFEB translocations constantly express PDL1., PMID:33208882

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545

Identification of miR-200a-5p targeting the peptide transporter TAP1 and its association with the clinical outcome of melanoma patients., PMID:32923135

The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses., PMID:32249716

Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation., PMID:32019420

Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease., PMID:31462148

Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits., PMID:31234698

ATG5 is required for B cell polarization and presentation of particulate antigens., PMID:30196744

Optimization of Recovery and Analysis of RNA in Sorted Cells in mRNA Quantification After Fluorescence-activated Cell Sorting., PMID:27993868

Clinical relevance of miR-mediated HLA-G regulation and the associated immune cell infiltration in renal cell carcinoma., PMID:26155421

Targeting syndecan-1 in breast cancer inhibits osteoclast functions through up-regulation of osteoprotegerin., PMID:26909290

DDX6 post-transcriptionally down-regulates miR-143/145 expression through host gene NCR143/145 in cancer cells., PMID:23932921

Env gene expression of human endogenous retrovirus-k and human endogenous retrovirus-w in childhood acute leukemia cells., PMID:23328642

Immunization with ovarian autoantigens leads to reduced fertility in mice following follicular dysfunction., PMID:22143970

Identification and validation of candidate biomarkers involved in human ovarian autoimmunity., PMID:21890413

Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids., PMID:20477829

An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV., PMID:20398263

Characterizing mouse male germ cell-specific actin capping protein alpha3 (CPalpha3): dynamic patterns of expression in testicular and epididymal sperm., PMID:18645673

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-ACTB/β-actin/Beta Actin Antibody (R3N80) [RHF37503]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only