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Anti-EIF5 Antibody (R3N31)

Catalog #:   RHF07502 Specific References (8) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: ELISA, FCM, IF, IHC
Accession: P55010
Overview

Catalog No.

RHF07502

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:250, IHC: 1:200-1:1000

Target

eIF-5, Eukaryotic translation initiation factor 5, EIF5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P55010

Applications

ELISA, FCM, IF, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N31

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using EIF5 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using EIF5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using EIF5 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using EIF5 mouse mAb with DAB staining.
References

Dysregulation of Translation Factors EIF2S1, EIF5A and EIF6 in Intestinal-Type Adenocarcinoma (ITAC)., PMID:34830804

A viral factor, CpBV15α, interacts with a translation initiation factor, eIF2, to suppress host gene expression at a post-transcriptional level., PMID:23711415

Biochemical characterization of mammalian translation initiation factor 3 (eIF3). Molecular cloning reveals that p110 subunit is the mammalian homologue of Saccharomyces cerevisiae protein Prt1., PMID:9388245

Characterization of mammalian translation initiation factor 5 (eIF-5). Demonstration that eIF-5 is a phosphoprotein and is present in cells as a single molecular form of apparent M(r) 58,000., PMID:8376415

Isolation and immunochemical characterization of eukaryotic translation initiation factor 5 from Saccharomyces cerevisiae., PMID:8449940

Function of eukaryotic initiation factor 5 in the formation of an 80 S ribosomal polypeptide chain initiation complex., PMID:1856230

Further characterization of eukaryotic initiation factor 5 from rabbit reticulocytes. Immunochemical characterization and phosphorylation by casein kinase II., PMID:2925684

Translational initiation factors from sea urchin eggs and embryos: functional properties are highly conserved., PMID:3767369

Datasheet

Document Download

Anti-EIF5 Antibody (R3N31).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-EIF5 Antibody (R3N31) [RHF07502]
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