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Anti-PRKAA2 Antibody (R3N18)

Catalog #:   RHF04003 Specific References (14) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P54646
Overview

Catalog No.

RHF04003

Species reactivity

Human, Mouse, Rat, Rabbit, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:500, IHC: 1:100-1:500, WB: 1:500-1:2000

Target

PRKAA2, Acetyl-CoA carboxylase kinase, AMPK subunit alpha-2, ACACA kinase, AMPK, AMPK2, Hydroxymethylglutaryl-CoA reductase kinase, 5'-AMP-activated protein kinase catalytic subunit alpha-2, HMGCR kinase

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P54646

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N18

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of NIH/3T3 cells using PRKAA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse kidney using PRKAA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse kidney using PRKAA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat kidney using PRKAA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit kidney using PRKAA2 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.
  • Western blot
    Western blot analysis using PRKAA2 mouse mAb against Mouse liver(1)PC-12(2)NIH/3T3(3)NRK(4)C2C12(5)C6(6)cell lysate.
References

4D label-free quantitative proteomic and phosphoproteomic profiling of the kidney in rat models of antimyeloperoxidase-associated vasculitis., PMID:40201969

Exploration and Identification of Vitamin D and Related Genes as Potential Biomarkers for Colorectal Tumors., PMID:39872437

AMPKα2 promotes tumor immune escape by inducing CD8+ T-cell exhaustion and CD4+ Treg cell formation in liver hepatocellular carcinoma., PMID:38424484

Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome., PMID:31398040

Autoantibody against β1-adrenoceptor promotes the differentiation of natural regulatory T cells from activated CD4+ T cells by up-regulating AMPK-mediated fatty acid oxidation., PMID:30770790

CaMKKβ-AMPKα2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells., PMID:25590814

AMPK/TSC2/mTOR-signaling intermediates are not necessary for LKB1-mediated nuclear retention of PTEN tumor suppressor., PMID:21123367

Ablation of AMP-activated protein kinase alpha1 and alpha2 from mouse pancreatic beta cells and RIP2.Cre neurons suppresses insulin release in vivo., PMID:20221584

Activation of AMP-activated protein kinase by vascular endothelial growth factor mediates endothelial angiogenesis independently of nitric-oxide synthase., PMID:20129920

Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle., PMID:19923418

Detection of AMP-activated protein kinase in human sera by immuno-isoelectric focusing., PMID:19818790

Long-term moderate ethanol consumption restores insulin sensitivity in high-fat-fed rats by increasing SLC2A4 (GLUT4) in the adipose tissue by AMP-activated protein kinase activation., PMID:18719073

Expression profiling of the gamma-subunit isoforms of AMP-activated protein kinase suggests a major role for gamma3 in white skeletal muscle., PMID:14559719

Metabolic and mitogenic signal transduction in human skeletal muscle after intense cycling exercise., PMID:12527721

Datasheet

Document Download

Anti-PRKAA2 Antibody (R3N18).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-PRKAA2 Antibody (R3N18) [RHF04003]
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