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Anti-ACLY Antibody (R3N03)

Catalog #:   RHE98003 Specific References (7) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P53396
Overview

Catalog No.

RHE98003

Species reactivity

Human, Mouse, Rat, Rabbit, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:500, IHC: 1:100-1:500, WB: 1:500-1:2000

Target

Citrate cleavage enzyme, ACLY, ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P53396

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N03

Data Image
  • Flow Cytometry
    Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of NIH/3T3 cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ACLY mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using ACLY mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using ACLY mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse liver(A) Mouse kidney(B) using ACLY mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat liver(A) Rat kidney(B) using ACLY mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit liver(A) Rabbit kidney(B) using ACLY mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
  • Western blot
    Western blot analysis using ACLY mouse mAb against PC-12(1)Raw264.7(2)NIH/3T3(3)NRK(4)C6(5)F9(6)COS-7(7)CHO3D10(8)cell lysate.
References

PI3Kβ functions as a protein kinase to promote cellular protein O-GlcNAcylation and acetyl-CoA production for tumor growth., PMID:40132583

IGFBP-3 promotes cachexia-associated lipid loss by suppressing insulin-like growth factor/insulin signaling., PMID:37014770

Exploring the possible neuroprotective and antioxidant potency of lycopene against acrylamide-induced neurotoxicity in rats' brain., PMID:33711552

Pharmacological induction of mesenchymal-epithelial transition via inhibition of H2S biosynthesis and consequent suppression of ACLY activity in colon cancer cells., PMID:33484818

The IKKβ-USP30-ACLY Axis Controls Lipogenesis and Tumorigenesis., PMID:32221968

Influence of elevated protein and tannin-rich peanut skin supplementation on growth performance, blood metabolites, carcass traits and immune-related gene expression of grazing meat goats., PMID:31724236

Cell surface GRP78 promotes tumor cell histone acetylation through metabolic reprogramming: a mechanism which modulates the Warburg effect., PMID:29296215

Datasheet

Document Download

Anti-ACLY Antibody (R3N03).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-ACLY Antibody (R3N03) [RHE98003]
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