Catalog No.
RHE93204
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IP: 1:20, WB: 1:500-1:1000
Target
EG5, TR-interacting protein 5, Kinesin-like protein KIF11, TRIP-5, KNSL1, Kinesin-like spindle protein HKSP, TRIP5, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, KIF11, Kinesin-like protein 1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P52732
Applications
IF, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3M90
Potent neutralization by a receptor binding domain monoclonal antibody with broad specificity for SARS-CoV-2 JN.1 and other variants., PMID:38746414
Cellular mechanisms associated with sub-optimal immune responses to SARS-CoV-2 bivalent booster vaccination in patients with Multiple Myeloma., PMID:37995467
NAT10 regulates mitotic cell fate by acetylating Eg5 to control bipolar spindle assembly and chromosome segregation., PMID:35210604
Discovery of Potent and Selective Antibody-Drug Conjugates with Eg5 Inhibitors through Linker and Payload Optimization., PMID:31857845
Filanesib for the treatment of multiple myeloma., PMID:31815551
Different Results of IgE Binding- and Crosslinking-Based Allergy Tests Caused by Allergen Immobilization., PMID:27725443
Targeted siRNA Delivery Using a Lipo-Oligoaminoamide Nanocore with an Influenza Peptide and Transferrin Shell., PMID:27109317
Phosphorylation of targeting protein for Xenopus kinesin-like protein 2 (TPX2) at threonine 72 in spindle assembly., PMID:25688093
Intracellular delivery of functionally active proteins using self-assembling pyridylthiourea-polyethylenimine., PMID:24476809
Eg5 restricts anaphase B spindle elongation in mammalian cells., PMID:24285623
Rotavirus viroplasm fusion and perinuclear localization are dynamic processes requiring stabilized microtubules., PMID:23110139
Development of hemiasterlin derivatives as potential anticancer agents that inhibit tubulin polymerization and synergize with a stilbene tubulin inhibitor., PMID:21655917
Inhibition of Kinesin-5, a microtubule-based motor protein, as a strategy for enhancing regeneration of adult axons., PMID:21166743
Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux., PMID:20110350
Interplay of microtubule dynamics and sliding during bipolar spindle formation in mammalian cells., PMID:19931454
Localization and function of Kinesin-5-like proteins during assembly and maintenance of mitotic spindles in Silvetia compressa., PMID:19527496
Truncated form of tenascin-X, XB-S, interacts with mitotic motor kinesin Eg5., PMID:18679583
Disruption of the mitotic kinesin Eg5 gene (Knsl1) results in early embryonic lethality., PMID:18474226
The novel combination of chlorpromazine and pentamidine exerts synergistic antiproliferative effects through dual mitotic action., PMID:18056463
Cytomegalovirus destruction of focal adhesions revealed in a high-throughput Western blot analysis of cellular protein expression., PMID:17522202
Essential roles for cohesin in kinetochore and spindle function in Xenopus egg extracts., PMID:17158911
Deciphering protein function during mitosis in PtK cells using RNAi., PMID:16796742
HURP is part of a Ran-dependent complex involved in spindle formation., PMID:16631581
Centrioles resist forces applied on centrosomes during G2/M transition., PMID:15898952
The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK., PMID:15302853
A novel action of terpendole E on the motor activity of mitotic Kinesin Eg5., PMID:12618185
Involvement of Aurora A kinase during meiosis I-II transition in Xenopus oocytes., PMID:12426316
Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport., PMID:12235119
The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle., PMID:10525540
BimC motor protein KLP61F cycles between mitotic spindles and fusomes in Drosophila germ cells., PMID:10469596
Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development., PMID:9742151
A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity., PMID:9707401
Opposing motor activities are required for the organization of the mammalian mitotic spindle pole., PMID:8896597
Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo., PMID:8548803
A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos., PMID:8083185
The kinesin-related protein Eg5 associates with both interphase and spindle microtubules during Xenopus early development., PMID:8026619
Mitotic spindle organization by a plus-end-directed microtubule motor., PMID:1406972
Distribution of T-cell receptor-bearing lymphocytes in the synovial membrane from patients with rheumatoid arthritis., PMID:2088392
For research use only. Not for human or drug use.
