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Anti-VASP Antibody (R3M32)

Catalog #:   RHE77502 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P50552
Overview

Catalog No.

RHE77502

Species reactivity

Human, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Vasodilator-stimulated phosphoprotein, VASP

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P50552

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3M32

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MOLT4 cells using VASP mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of THP-1 cells using VASP mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of HL-60 cells using VASP mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using VASP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using VASP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using VASP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using VASP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using VASP mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using VASP mouse mAb against THP-1 (1), HepG2 (2), Hela (3), HT-29 (4), MCF-7 (5), A549 (6), and COS-7 (7) cell lysate.
References

Unveiling the impact of Cancer-IgG on glioma: Insights into biological behavior and macrophage polarization dynamics., PMID:39395381

Anti-vasodilator-stimulated phosphoprotein (VASP) antibodies are associated with neuropsychiatric disorders in systemic lupus erythematosus., PMID:39296110

Leishmania donovani mevalonate kinase regulates host actin for inducing phagocytosis., PMID:38123120

A prospective observational phase IV study on effectiveness of animal derived polyclonal antibody antivenoms against West African carpet viper (Echis romani) induced coagulopathy and mortality., PMID:37393958

Pre-clinical efficacy of African medicinal plants used in the treatment of snakebite envenoming: A systematic review., PMID:36706926

Platelets and the Cybernetic Regulation of Ischemic Inflammatory Responses through PNC Formation Regulated by Extracellular Nucleotide Metabolism and Signaling., PMID:36230973

Preclinical efficacy of African medicinal plants used in the treatment of snakebite envenoming: a systematic review protocol., PMID:35237433

Influence of long-term proteasome inhibition on platelet responsiveness mediated by bortezomib., PMID:33422688

Challenges and prospects of snake antivenom supply in sub-Saharan Africa., PMID:32817674

NKG2D-DAP10 signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity., PMID:31235500

The role of NO-cGMP pathway inhibition in vascular endothelial-dependent smooth muscle relaxation disorder of AT1-AA positive rats: protective effects of adiponectin., PMID:30831264

Role of Corneal Stromal Cells on Epithelial Cell Function during Wound Healing., PMID:29401709

Effectiveness and safety of ustekinumab in naïve or TNF-inhibitors failure psoriatic arthritis patients: a 24-month prospective multicentric study., PMID:29302829

Detrimental roles of TNF-alpha in the antiphospholipid syndrome and de novo synthesis of antiphospholipid antibodies induced by biopharmaceuticals against TNF-alpha., PMID:29027631

The Ig VH complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor., PMID:27575453

Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes., PMID:26169271

The Ultrastructure of MCF-7 Breast Cancer Cells after Vasodilator-Stimulated Phosphoprotein Knockdown., PMID:26107555

hMENA(11a) contributes to HER3-mediated resistance to PI3K inhibitors in HER2-overexpressing breast cancer cells., PMID:25961924

The nitric oxide donor pentaerythritol tetranitrate reduces platelet activation in congestive heart failure., PMID:25928879

Epithelial-specific A2B adenosine receptor signaling protects the colonic epithelial barrier during acute colitis., PMID:25850656

A modular toolkit to inhibit proline-rich motif-mediated protein-protein interactions., PMID:25848013

Anchoring of protein kinase A by ERM (ezrin-radixin-moesin) proteins is required for proper netrin signaling through DCC (deleted in colorectal cancer)., PMID:25575591

Detection of clopidogrel resistance using ADP induced aggregometry with specific inhibitor PGE1., PMID:25291943

Multiplexed phosphospecific flow cytometry enables large-scale signaling profiling and drug screening in blood platelets., PMID:25056834

Staphylococcus aureus lipoteichoic acid inhibits platelet activation and thrombus formation via the Paf receptor., PMID:23911710

Structure and function of palladin's actin binding domain., PMID:23806659

β1-Adrenoceptor autoantibodies from DCM patients enhance the proliferation of T lymphocytes through the β1-AR/cAMP/PKA and p38 MAPK pathways., PMID:23300817

Quantitative assessment of invasive mena isoforms (Menacalc) as an independent prognostic marker in breast cancer., PMID:22971274

β1 -adrenergic receptor autoantibodies from heart failure patients enhanced TNF-α secretion in RAW264.7 macrophages in a largely PKA-dependent fashion., PMID:22628174

Monomeric C-reactive protein is prothrombotic and dissociates from circulating pentameric C-reactive protein on adhered activated platelets under flow., PMID:21859817

Identification of Fyn as the binding partner for the WASP N-terminal domain in T cells., PMID:21705469

Thrombospondin-1 induces platelet activation through CD36-dependent inhibition of the cAMP/protein kinase A signaling cascade., PMID:20664056

Zyxin controls migration in epithelial-mesenchymal transition by mediating actin-membrane linkages at cell-cell junctions., PMID:19927303

Differential VASP phosphorylation controls remodeling of the actin cytoskeleton., PMID:19825941

The immunohistochemical aspects of protein Mena in cervical lesions., PMID:19434313

The expression of cytoskeleton regulatory protein Mena in colorectal lesions., PMID:18758639

The profilin:actin complex localizes to sites of dynamic actin polymerization at the leading edge of migrating cells and pathogen-induced actin tails., PMID:18707793

Protein kinase G phosphorylates soluble guanylyl cyclase on serine 64 and inhibits its activity., PMID:18635821

The nitration of platelet vasodilator stimulated phosphoprotein following exposure to low concentrations of hydrogen peroxide., PMID:18569864

Carbon monoxide and nitric oxide mediate cytoskeletal reorganization in microvascular cells via vasodilator-stimulated phosphoprotein phosphorylation: evidence for blunted responsiveness in diabetes., PMID:18559661

IL-5 and eosinophilia., PMID:18511250

Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila., PMID:17959833

Hearing development and spiral ganglion neurite growth in VASP deficient mice., PMID:17920567

Regulation of VASP serine 157 phosphorylation in human neutrophils after stimulation by a chemoattractant., PMID:17684042

The flow cytometric VASP assay can be used to determine the effectiveness of clopidogrel in patients treated with abciximab., PMID:17263784

Ena/VASP proteins mediate repulsion from ephrin ligands., PMID:17179204

AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein., PMID:17082196

PREL1 provides a link from Ras signalling to the actin cytoskeleton via Ena/VASP proteins., PMID:16783873

Podosomes as smart regulators of cellular adhesion., PMID:16546561

Eve-3: a liver enriched suppressor of Ras/MAPK signaling., PMID:16478641

Datasheet

Document Download

Anti-VASP Antibody (R3M32).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-VASP Antibody (R3M32) [RHE77502]
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