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Anti-MAP2K3 Antibody (R3L55)

Catalog #:   RHE48804 Specific References (24) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC
Accession: P46734
Overview

Catalog No.

RHE48804

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000

Target

SAPK kinase 2, PRKMK3, Dual specificity mitogen-activated protein kinase kinase 3, MAPKK 3, SAPKK-2, MAP2K3, SKK2, MAP kinase kinase 3, SAPKK2, MEK3, Stress-activated protein kinase kinase 2, MKK3, MAPK/ERK kinase 3, MEK 3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P46734

Applications

ELISA, FCM, IF, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3L55

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using MAP2K3 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of GC-7901 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of HepG2 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using MAP2K3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using MAP2K3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using MAP2K3 mouse mAb with DAB staining.
References

Pharmacogenomics of intravenous immunoglobulin response in Kawasaki disease., PMID:38259468

Peripheral blood mononuclear cell- transcriptome signatures of atopic dermatitis and prediction for the efficacy of dupilumab., PMID:37349237

Blockade of CD26 signaling inhibits human osteoclast development., PMID:24821427

Stem cell factor induces AP-1-dependent mast cell IL-6 production via MAPK kinase 3 activity., PMID:24453276

HDAC inhibition elicits myocardial protective effect through modulation of MKK3/Akt-1., PMID:23762381

Stress-activated kinase pathway alteration is a frequent event in bladder cancer., PMID:22154358

Decreased collagen-induced arthritis severity and adaptive immunity in MKK-6-deficient mice., PMID:21953132

Recent insights into diabetic renal injury from the db/db mouse model of type 2 diabetic nephropathy., PMID:21147843

ß-Elemene inhibits proliferation of human glioblastoma cells and causes cell-cycle G0/G1 arrest via mutually compensatory activation of MKK3 and MKK6., PMID:21132268

Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation., PMID:17214902

Inactivation of the Akt survival pathway during photoreceptor apoptosis in the retinal degeneration mouse., PMID:16565401

The volatile anesthetics halothane and isoflurane differentially modulate proinflammatory cytokine-induced p38 mitogen-activated protein kinase activation., PMID:15290420

p38 MAPK is a critical regulator of the constitutive and the beta4 integrin-regulated expression of IL-6 in human normal thymic epithelial cells., PMID:14579272

Sustained activation of JNK/p38 MAPK pathways in response to cisplatin leads to Fas ligand induction and cell death in ovarian carcinoma cells., PMID:12637505

The sesquiterpene lactone parthenolide inhibits LPS- but not TNF-alpha-induced maturation of human monocyte-derived dendritic cells by inhibition of the p38 mitogen-activated protein kinase pathway., PMID:12170268

Regulation of mediator secretion in human basophils by p38 mitogen-activated protein kinase: phosphorylation is sensitive to the effects of phosphatidylinositol 3-kinase inhibitors and calcium mobilization., PMID:12149431

Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2., PMID:11279118

Hyperosmotic stress stimulates promoter activity and regulates cellular utilization of the serum- and glucocorticoid-inducible protein kinase (Sgk) by a p38 MAPK-dependent pathway., PMID:10842172

Cell cycle arrest and reversion of Ras-induced transformation by a conditionally activated form of mitogen-activated protein kinase kinase kinase 3., PMID:10207109

Human mitogen-activated protein kinase kinase kinase mediates the stress-induced activation of mitogen-activated protein kinase cascades., PMID:9841871

Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6., PMID:9430721

Expression, purification, and characterization of an activated cytokine-suppressive anti-inflammatory drug-binding protein 2 (CSBP2) kinase from baculovirus-infected insect cells., PMID:9226723

A novel kinase cascade mediated by mitogen-activated protein kinase kinase 6 and MKK3., PMID:8663074

Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase., PMID:8621389

Datasheet

Document Download

Anti-MAP2K3 Antibody (R3L55).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-MAP2K3 Antibody (R3L55) [RHE48804]
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