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Anti-FEN1 Antibody (R3K20)

Catalog #:   RHE24403 Specific References (18) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IP, WB
Accession: P39748
Overview

Catalog No.

RHE24403

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IP: 1:20, WB: 1:500-1:1000

Target

FEN-1, Maturation factor 1, hFEN-1, DNase IV, RAD2, FEN1, Flap endonuclease 1, MF1, Flap structure-specific endonuclease 1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P39748

Applications

IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3K20

Data Image
  • Immunoprecipitation
    Immunoprecipitation analysis of FEN1 in Hela lysates using FEN1 antibody.
  • Western blot
    Western blot analysis of FEN1 in Hela, Jurkat, 3T3, C6, CEM and Ramos lysates using FEN1 antibody.
  • Western blot
    Western blot analysis of FEN1 in Hela, Jurkat, K562 and 3T3 lysates using FEN1 antibody.
References

A reduced proteomic signature in critically ill Covid-19 patients determined with plasma antibody micro-array and machine learning., PMID:38760690

Fenofibrate attenuates asthma features in an ovalbumin-induced mouse model via suppressing NF-κB binding activity., PMID:37295485

Photo-affinity pulling down of low-affinity binding proteins mediated by post-translational modifications., PMID:32200891

Lysine methylation of FEN1 by SET7 is essential for its cellular response to replicative stress., PMID:29029401

Cell Killing Mechanisms and Impact on Gene Expression by Gemcitabine and 212Pb-Trastuzumab Treatment in a Disseminated i.p. Tumor Model., PMID:27467592

A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes., PMID:27141097

A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells., PMID:25879709

Crystal structure of the VapBC-15 complex from Mycobacterium tuberculosis reveals a two-metal ion dependent PIN-domain ribonuclease and a variable mode of toxin-antitoxin assembly., PMID:25450593

Impact of α-targeted radiation therapy on gene expression in a pre-clinical model for disseminated peritoneal disease when combined with paclitaxel., PMID:25268703

Human MUS81 complexes stimulate flap endonuclease 1., PMID:22551069

Long patch base excision repair in mammalian mitochondrial genomes., PMID:18635552

Partial reconstitution of DNA large loop repair with purified proteins from Saccharomyces cerevisiae., PMID:18628298

Heat shock does not induce gammaH2AX foci formation but protects cells from N-methyl-N'-nitro-N-nitrosoguanidine-induced genotoxicity., PMID:17276133

Characterization of plant proliferating cell nuclear antigen (PCNA) and flap endonuclease-1 (FEN-1), and their distribution in mitotic and meiotic cell cycles., PMID:11851910

Fen1 expression: a novel marker for cell proliferation., PMID:10211123

Processing of branched DNA intermediates by a complex of human FEN-1 and PCNA., PMID:8668533

Ovarian cancer-associated antibodies recovered from ascites: their use for the isolation of ovarian cancer-associated antigen to produce monoclonal antibodies., PMID:2182406

Immunohistochemical characterization of a monoclonal antibody detecting an endometrioid ovarian cancer-associated antigen., PMID:2197240

Datasheet

Document Download

Anti-FEN1 Antibody (R3K20).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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For research use only. Not for human or drug use.

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Anti-FEN1 Antibody (R3K20) [RHE24403]
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