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Anti-PHB Antibody (R3J73)

Catalog #:   RHE08102 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P35232
Overview

Catalog No.

RHE08102

Species reactivity

Human, Mouse, Rat, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Prohibitin, PHB, PHB1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P35232

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3J73

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PHB mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using PHB mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PHB mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PHB mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PHB mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.
References

Evaluation of the hematological parameters, inflammatory biomarkers, and thyroid hormones in hypothyroidism patients., PMID:39731180

Bioinformatic and functional analysis of a PHB polymerase (PhbC) from Azospirillum baldaniorum., PMID:39179320

A stagewise response to mitochondrial dysfunction in mitochondrial DNA maintenance disorders., PMID:38521420

Nur77 increases mitophagy and decreases aggregation of α-synuclein by modulating the p-c-Abl/p-PHB2 Y121 in α-synuclein PFF SH-SY5Y cells and mice., PMID:38422699

Galectin-3 and prohibitin 1 are autoantigens in IgG4-related cholangitis without clear-cut protective effects against toxic bile acids., PMID:38332916

A comprehensive investigation to the fate of phosphorus in full-scale wastewater treatment plants using aluminum salts for enhanced phosphorus removal., PMID:38159765

One-step selective layer assemble: A versatile approach for the development of a SARS-CoV-2 electrochemical immunosensor., PMID:37709467

Transcriptome alterations in peripheral blood B cells of patients with multiple sclerosis receiving immune reconstitution therapy., PMID:37533036

Abatacept as Monotherapy and in Combination With Methotrexate in Patients With Juvenile Idiopathic Arthritis: Analysis of 2 Phase III Trials., PMID:37453737

Identification of Heterophilic Epitopes of H1N1 Influenza Virus Hemagglutinin., PMID:37074450

Prohibitin mediates the cellular invasion of spring viremia of the carp virus., PMID:36931480

Novel approach based on GQD-PHB as anchoring platform for the development of SARS-CoV-2 electrochemical immunosensor., PMID:36257733

A Preliminary, Single-Center Retrospective Chart Review of Infusion Times of Intravenous Immune Globulin in Kawasaki Disease and Clinical Outcomes., PMID:35845563

Non-Specific Binding and Cross-Reaction of ELISA: A Case Study of Porcine Hemoglobin Detection., PMID:34441486

Electrochemical Detection of Global DNA Methylation Using Biologically Assembled Polymer Beads., PMID:34359688

Bioengineered Polymer Nanobeads for Isolation and Electrochemical Detection of Cancer Biomarkers., PMID:34185493

Comparing RECIST 1.1 and iRECIST in advanced melanoma patients treated with pembrolizumab in a phase II clinical trial., PMID:32995974

Prohibitin (PHB) interacts with AKT in mitochondria to coordinately modulate sperm motility., PMID:32859869

Performance of the 2019 ACR/EULAR classification criteria for IgG4-related disease and clinical phenotypes in a Spanish multicentre registry (REERIGG4)., PMID:32658963

Monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for porcine hemoglobin quantification., PMID:32344349

Cell Surface Display of Poly(3-hydroxybutyrate) Depolymerase and its Application., PMID:32066215

The M4 insulator, the TM2 matrix attachment region, and the double copy of the heavy chain gene contribute to the enhanced accumulation of the PHB-01 antibody in tobacco plants., PMID:31919795

Outer membrane protein I is associated with poly-β-hydroxybutyrate granules and is necessary for optimal polymer accumulation in Azotobacter vinelandii on solid medium., PMID:31329095

Novel Silyl Ether-Based Acid-Cleavable Antibody-MMAE Conjugates with Appropriate Stability and Efficacy., PMID:31288450

Emerging views of mitophagy in immunity and autoimmune diseases., PMID:30951392

Tissue Expression of Prohibition-I and It's Relationship With Prognostic Factors in Breast Cancer., PMID:30697295

Targeting the hepcidin-ferroportin pathway in anaemia of chronic kidney disease., PMID:30677788

The mitochondrial chaperone Prohibitin 1 negatively regulates interleukin-8 in human liver cancers., PMID:30523154

Design and Biological Assembly of Polyester Beads Displaying Pneumococcal Antigens as Particulate Vaccine., PMID:33435075

Monoclonal antibody-based ELISA for the quantification of porcine hemoglobin in meat products., PMID:29412908

Significance of elevated Prohibitin 1 levels in Multiple Sclerosis patients lymphocytes towards the assessment of subclinical disease activity and its role in the central nervous system pathology of disease., PMID:29242126

Recombinant prohibitin protein of Leishmania infantum acts as a vaccine candidate and diagnostic marker against visceral leishmaniasis., PMID:29128045

Polymethacrylate Coated Electrospun PHB Fibers as a Functionalized Platform for Bio-Diagnostics: Confirmation Analysis on the Presence of Immobilized IgG Antibodies against Dengue Virus., PMID:28991214

Self-assembled particulate PsaA as vaccine against Streptococcus pneumoniae infection., PMID:28435909

Minimal change nephrotic syndrome and prohibitin-2 gene polymorphism., PMID:27812762

Epicutaneous immunotherapy induces gastrointestinal LAP+ regulatory T cells and prevents food-induced anaphylaxis., PMID:27417020

[The Analysis for Probable Reasons of Cd4+ T-Cell Activation Non-Linear Dependence on Extra Cellular Calcium Ion Concentration in Human Peripheral Blood in vitro]., PMID:26615640

Characterization of Prohibitin 1 as a Host Partner of Vibrio vulnificus RtxA1 Toxin., PMID:26136468

Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors., PMID:25765434

Mitochondrial prohibitin and its ubiquitination during crayfish Procambarus clarkii spermiogenesis., PMID:25418137

New skin test for detection of bovine tuberculosis on the basis of antigen-displaying polyester inclusions produced by recombinant Escherichia coli., PMID:24532066

Prohibitin 2 represents a novel nuclear AKT substrate during all-trans retinoic acid-induced differentiation of acute promyelocytic leukemia cells., PMID:24522204

Multi-stage continuous high cell density culture systems: a review., PMID:24462363

Immunostimulation by poly-β hydroxybutyrate-hydroxyvalerate (PHB-HV) from Bacillus thuringiensis in Oreochromis mossambicus., PMID:24161774

The scaffold protein prohibitin is required for antigen-stimulated signaling in mast cells., PMID:24023254

A role for prohibitin in mast cell activation: location matters., PMID:24023253

Characterization of Cherax quadricarinatus prohibitin and its potential role in spermatogenesis., PMID:23485620

Identification of prohibitin as a Chikungunya virus receptor protein., PMID:22997079

Characterization of a gene encoding prohibitin in silkworm, Bombyx mori., PMID:22450364

Prohibitins are involved in protease-activated receptor 1-mediated platelet aggregation., PMID:22212092

Datasheet

Document Download

Anti-PHB Antibody (R3J73).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-PHB Antibody (R3J73) [RHE08102]
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