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Anti-BMI1 Antibody (R3J71)

Catalog #:   RHE07802 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC
Accession: P35226
Overview

Catalog No.

RHE07802

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000

Target

RING finger protein 51, BMI1, RNF51, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, PCGF4

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P35226

Applications

ELISA, FCM, IF, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3J71

Data Image
  • Flow Cytometry
    Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BMI1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using BMI1 mouse mAb with DAB staining.
References

Thymic Bmi-1 hampers γδT17 generation and its derived RORγt-IL-17A signaling to delay cardiac aging., PMID:40366697

The transcription repressor Bach2 is required for maintaining the B-1 cell population by regulating self-renewal., PMID:40170867

Hedgehog Pathway Is a Regulator of Stemness in HER2-Positive Trastuzumab-Resistant Breast Cancer., PMID:39596169

Antibody conjugated targeted nanotherapy epigenetically inhibits calpain-mediated mitochondrial dysfunction to attenuate Parkinson's disease., PMID:39245478

Characterization of immortalized ovarian epithelial cells with BRCA1/2 mutation., PMID:38615309

Correlation between intestinal stem cell niche changes and small bowel crypt failure in patients with treatment-naïve celiac disease., PMID:38427764

BMI1 regulates human erythroid self-renewal through both gene repression and gene activation., PMID:38370741

The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation., PMID:37901870

Targeting BMI-1 to deplete antibody-secreting cells in autoimmunity., PMID:37799772

SOX18 meditates the resistance of Bmi1-expressing cells to cetuximab in HNSCC., PMID:37184032

Chondroitin Sulphate/Dermatan Sulphate Proteoglycans: Potential Regulators of Corneal Stem/Progenitor Cell Phenotype In Vitro., PMID:36768414

Age disparities in intestinal stem cell quantities: a possible explanation for preterm infant susceptibility to necrotizing enterocolitis., PMID:36208323

Targeting BMI-1 in B cells restores effective humoral immune responses and controls chronic viral infection., PMID:34845392

MiR-342-3p inhibits LCSC oncogenicity and cell stemness through HDAC7/PTEN axis., PMID:34842937

Targeting LSD1 suppresses stem cell-like properties and sensitizes head and neck squamous cell carcinoma to PD-1 blockade., PMID:34689153

The IL-6R and Bmi-1 axis controls self-renewal and chemoresistance of head and neck cancer stem cells., PMID:34689150

Administration of cyclophosphamide to rats induces pica and potentiates 5-hydroxytryptamine synthesis in the intestine without causing severe intestinal injury., PMID:34507634

Endoplasmic reticulum stress inhibits 3D Matrigel-induced vasculogenic mimicry of breast cancer cells via TGF-β1/Smad2/3 and β-catenin signaling., PMID:34320274

CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance., PMID:33945793

hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination., PMID:33916959

Upregulating hsa-miR-128a Increased the Effects of Pembrolizumab on Laryngeal Cancer Cells via the p53 Pathway., PMID:33791361

The effect of fibroblast growth factor 7 on human dental pulp stem cells for differentiation to AQP5-positive and αSMA-positive cells in vitro and in vivo., PMID:33783980

G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease., PMID:33758195

IL-21 Promotes Intestinal Memory IgA Responses., PMID:32859726

Stem Cell Marker Expression in Early Stage Colorectal Cancer is Associated with Recurrent Intestinal Neoplasia., PMID:32647988

Genomic and epigenomic insights into the origin, pathogenesis, and clinical behavior of mantle cell lymphoma subtypes., PMID:32584970

IL1R2 Blockade Suppresses Breast Tumorigenesis and Progression by Impairing USP15-Dependent BMI1 Stability., PMID:31921558

Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer., PMID:31897209

Precision medicine for human cancers with Notch signaling dysregulation (Review)., PMID:31894255

Shark tooth regeneration reveals common stem cell characters in both human rested lamina and ameloblastoma., PMID:31685919

E-cadherin-Fc chimera protein matrix enhances cancer stem-like properties and induces mesenchymal features in colon cancer cells., PMID:31505062

Antibody-drug conjugate T-DM1 treatment for HER2+ breast cancer induces ROR1 and confers resistance through activation of Hippo transcriptional coactivator YAP1., PMID:31085100

The expression and prognostic value of stem cell markers Bmi-1, HESC5:3, and HES77 in human papillomavirus-positive and -negative oropharyngeal squamous cell carcinoma., PMID:30915904

Transformation of mature mouse B cells into malignant plasma cells in vitro via introduction of defined genetic elements., PMID:30664244

Inhibition of chemotherapy resistant breast cancer stem cells by a ROR1 specific antibody., PMID:30622177

The Polycomb BMI1 Protein Is Co-expressed With CD26+ in Leukemic Stem Cells of Chronic Myeloid Leukemia., PMID:30574454

Gankyrin Contributes to Tumorigenesis and Chemoresistance in Sporadic Colorectal Cancer., PMID:30513515

Combined detection of serum autoantibodies as diagnostic biomarkers in esophagogastric junction adenocarcinoma., PMID:30426295

Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma., PMID:30297829

Bevacizumab with dose-dense paclitaxel/carboplatin as first-line chemotherapy for advanced ovarian cancer., PMID:30059681

Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5- and Bmi1-Expressing Intestinal Cell Populations., PMID:29928673

Effect of Bmi-1-mediated NF-κB signaling pathway on the stem-like properties of CD133+ human liver cancer cells., PMID:29843222

Abundant expression of BMI1 in follicular lymphoma is associated with reduced overall survival., PMID:29251058

Bmi‑1‑targeting suppresses osteosarcoma aggressiveness through the NF‑κB signaling pathway., PMID:28983587

Bispecific antibody suppresses osteosarcoma aggressiveness through regulation of NF-κB signaling pathway., PMID:28631557

Intestinal Epithelial Stem Cells: Distinct Behavior After Surgical Injury and Teduglutide Administration., PMID:28362133

MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1., PMID:28098896

Identification, expansion and characterization of cancer cells with stem cell properties from head and neck squamous cell carcinomas., PMID:27619333

Heterogeneity in readouts of canonical wnt pathway activity within intestinal crypts., PMID:27264700

Bmi1 inhibition enhances the sensitivity of pancreatic cancer cells to gemcitabine., PMID:27177084

Datasheet

Document Download

Anti-BMI1 Antibody (R3J71).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-BMI1 Antibody (R3J71) [RHE07802]
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