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Anti-HSPA4/HSP70 Antibody (R3J60)

Catalog #:   RHE05803 Specific References (13) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IHC, WB
Accession: P34932
Overview

Catalog No.

RHE05803

Species reactivity

Human, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Heat shock 70-related protein APG-2, APG2, HSP70RY, Heat shock 70 kDa protein 4, HSPA4

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P34932

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3J60

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using HSP70 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using HSP70 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using HSP70 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using HSP70 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using HSP70 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSP70 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using HSP70 mouse mAb against NIH/3T3 (1), Hela (2), HepG2 (3), Hek293 (4), COS-7 (5), A549 (6), and Jurkat (7) cell lysate.
References

Integrative analysis of serum proteomics and transcriptomics in hepatitis C., PMID:40082995

HSPA4 upregulation induces immune evasion via ALKBH5/CD58 axis in gastric cancer., PMID:38589927

The antimicrobial protein S100A12 identified as a potential autoantigen in a subgroup of atopic dermatitis patients., PMID:30728947

Tumor-educated B cells selectively promote breast cancer lymph node metastasis by HSPA4-targeting IgG., PMID:30643287

Enteral glutamine infusion modulates ubiquitination of heat shock proteins, Grp-75 and Apg-2, in the human duodenal mucosa., PMID:24449167

Screening of autoantibodies as potential biomarkers for hepatocellular carcinoma by using T7 phase display system., PMID:22018955

Overexpression of Apg-2 increases cell proliferation and protects from oxidative damage in BaF3-BCR/ABL cells., PMID:20198334

High-content functional screen to identify proteins that correct F508del-CFTR function., PMID:19088066

Identification of an overexpressed gene, HSPA4L, the product of which can provoke prevalent humoral immune responses in leukemia patients., PMID:17588478

Cleavage at the carboxyl-terminus of Ku80 during apoptosis in human Jurkat T cells., PMID:16002990

Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes., PMID:15897011

Gene cloning of immunogenic antigens overexpressed in pancreatic cancer., PMID:11237751

Expression of APG-2 protein, a member of the heat shock protein 110 family, in developing rat brain., PMID:10566957

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-HSPA4/HSP70 Antibody (R3J60) [RHE05803]
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