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Anti-CHRNA3 Antibody (R3J37)

Catalog #:   RHD99701 Specific References (44) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF
Accession: P32297
Overview

Catalog No.

RHD99701

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000

Target

NACHRA3, Neuronal acetylcholine receptor subunit alpha-3, CHRNA3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P32297

Applications

ELISA, FCM, IF

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3J37

Data Image
  • Flow Cytometry
    Flow cytometric analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
References

Nicotinic Acetylcholine Receptor Expression in Merkel Cell Carcinoma Is Associated With Clinical and Histopathologic Parameters., PMID:39814046

Abundant extrasynaptic expression of α3β4-containing nicotinic acetylcholine receptors in the medial habenula-interpeduncular nucleus pathway in mice., PMID:38902419

Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets., PMID:38155190

Disconnected Cardiac Autonomic Nerves in Genetic Ganglionic Acetylcholine Receptor Alpha-3 Subunit Deficiency., PMID:37161764

Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets., PMID:36798270

Antibodies to the α3 subunit of the ganglionic-type nicotinic acetylcholine receptors in patients with autoimmune encephalitis., PMID:32980672

Case of autoimmune autonomic ganglionopathy manifesting anhidrosis., PMID:28620980

Adult-onset Opsoclonus-Myoclonus Syndrome Associated With Ganglionic Acetylcholine Receptor Autoantibody., PMID:27801769

Regulation of Tlx3 by Pax6 is required for the restricted expression of Chrnα3 in Cerebellar Granule Neuron progenitors during development., PMID:27452274

Association between Anti-Ganglionic Nicotinic Acetylcholine Receptor (gAChR) Antibodies and HLA-DRB1 Alleles in the Japanese Population., PMID:26807576

Cholinergic epithelial cell with chemosensory traits in murine thymic medulla., PMID:25300645

CHRNA3 polymorphism modifies lung adenocarcinoma risk in the Chinese Han population., PMID:24686516

Autoantibody-induced internalization of nicotinic acetylcholine receptor α3 subunit exogenously expressed in human embryonic kidney cells., PMID:23313381

Treatment-responsive pandysautonomia in an adolescent with ganglionic α3-AChR antibodies., PMID:22130491

Efficacy of immunotherapy in seropositive and seronegative putative autoimmune autonomic ganglionopathy., PMID:19506222

Ganglionic acetylcholine receptor autoantibody: oncological, neurological, and serological accompaniments., PMID:19506133

Special section on autoimmune autonomic ganglionopathy. Editorial., PMID:19217830

A Japanese case of autoimmune autonomic ganglionopathy (AAG) and a review of AAG cases in Japan., PMID:19195935

Clinical laboratory evaluation of autoimmune autonomic ganglionopathy: Preliminary observations., PMID:19155193

Antibody titers predict clinical features of autoimmune autonomic ganglionopathy., PMID:19144572

Autoimmune autonomic ganglionopathy with Sjögren's syndrome: significance of ganglionic acetylcholine receptor antibody and therapeutic approach., PMID:19097826

Other autonomic neuropathies associated with ganglionic antibody., PMID:19058765

Immunotherapy for autoimmune autonomic ganglionopathy., PMID:19056323

Autoimmune autonomic ganglionopathy with late-onset encephalopathy., PMID:19028442

Autonomic ganglia, acetylcholine receptor antibodies, and autoimmune ganglionopathy., PMID:18951069

Functional interactions between the SK2 channel and the nicotinic acetylcholine receptor in enteric neurons of the guinea pig ileum., PMID:17953675

Nicotinic acetylcholine receptors containing subunits alpha3 and alpha5 in rat nociceptive dorsal root ganglion neurons., PMID:17192625

The high-affinity choline transporter: a critical protein for sustaining cholinergic signaling as revealed in studies of genetically altered mice., PMID:16722248

Nicotinic acetylcholine receptor subunits and associated proteins in human sperm., PMID:15894803

Passive transfer of autoimmune autonomic neuropathy to mice., PMID:15306637

Production of antibodies to alpha(181-192) peptides of neuronal nicotinic acetylcholine receptor coupled to protein carriers in different orientations., PMID:14964862

Distribution of neuronal nicotinic acetylcholine receptors containing different alpha-subunits in the submucosal plexus of the guinea-pig., PMID:14766321

Increased exchange rate of histone H1 on chromatin by exogenous myogenin expression., PMID:12528897

Dystrophin stabilizes alpha 3- but not alpha 7-containing nicotinic acetylcholine receptor subtypes at the postsynaptic apparatus in the mouse superior cervical ganglion., PMID:12079404

Expression of nicotinic receptors in the skin of patients with palmoplantar pustulosis., PMID:11952537

Changes in nicotinic acetylcholine receptor subunits expression in brain of patients with Down syndrome and Alzheimer's disease., PMID:11771745

Structure of epitopes recognized by the antibodies to alpha(181-192) peptides of neuronal nicotinic acetylcholine receptors: extrapolation to the structure of acetylcholine-binding domain., PMID:11730940

Nicotinic acetylcholine receptor subtypes in rat superior cervical ganglion neurons as studied by sequential application of two alpha-subunit-specific antibodies., PMID:11297818

Neuronal nicotinic acetylcholine receptor alpha3 subunit protein in rat brain and sympathetic ganglion measured using a subunit-specific antibody: regional and ontogenic expression., PMID:11279289

Anti-neuronal nicotinic receptor antibodies in MG patients with thymoma., PMID:11137585

Neuronal nicotinic acetylcholine receptors of Drosophila melanogaster: the alpha-subunit dalpha3 and the beta-type subunit ARD co-assemble within the same receptor complex., PMID:11024458

Alpha subunit composition of nicotinic acetylcholine receptors in the rat autonomic ganglia neurons as determined with subunit-specific anti-alpha(181-192) peptide antibodies., PMID:10501468

Alpha4 but not alpha3 and alpha7 nicotinic acetylcholine receptor subunits are lost from the temporal cortex in Alzheimer's disease., PMID:10501210

Fast excitatory nicotinic transmission in the chick lateral spiriform nucleus., PMID:10479683

Datasheet

Document Download

Anti-CHRNA3 Antibody (R3J37).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-CHRNA3 Antibody (R3J37) [RHD99701]
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