Catalog No.
RHD96512
Species reactivity
Human, Rat, Monkey
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
RAC-alpha serine/threonine-protein kinase, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene c-Akt, Protein kinase B alpha, Protein kinase B, AKT1, PKB
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P31749
Applications
ELISA, FCM, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3J17
Vitamin D and Inhibition of Melanogenesis: Examination of Liposomal Vitamin D Nanocarrier Anti-Melanogenesis Activity on B16 F10 Cell Line., PMID:40390845
Novel Estrogen Receptor - Targeted Therapies in Hormone-Receptor Positive Breast Cancer., PMID:40163189
Oleanolic acid inhibits mesangial cell proliferation and inflammatory response in mesangial proliferative glomerulonephritis through IL-17/ERK/AKT pathway., PMID:40088868
Elucidating the pharmacological foundations and mechanisms of the Sihai Shuyu formula in treating Graves' disease through integrated serum metabolomics and network pharmacology with molecular docking techniques., PMID:39950029
Inhibition of Kinase Activity and In Vitro Downregulation of the Protein Kinases in Lung Cancer and Cervical Cancer Cell Lines and the Identified Known Anticancer Compounds of Ziziphus mucronata., PMID:39942957
Jian Pi Hua Tan Fang Reverses Trastuzumab Resistance of HER2-Positive Gastric Cancer Through PI3K/AKT/mTOR Pathway: Integrating Network Pharmacology, Molecular Docking and Experimental Validation., PMID:39917999
Engineered FHA domains can bind to a variety of Phosphothreonine-containing peptides., PMID:39276365
Insights into the role of FoxL2 in tebuconazole-induced male- biased sex differentiation of zebrafish., PMID:38977095
SUMOylation of annexin A6 retards cell migration and tumor growth by suppressing RHOU/AKT1-involved EMT in hepatocellular carcinoma., PMID:38566133
The Combination of Afatinib With Dasatinib or Miransertib Results in Synergistic Growth Inhibition of Stomach Cancer Cells., PMID:38545471
Effects of Viscum coloratum (Kom.) Nakai on collagen-induced rheumatoid arthritis., PMID:38490288
Molecular screening with liquid biopsy for anti-EGFR retreatment in metastatic colorectal cancer: preliminary data from the randomized phase 2 PARERE trial., PMID:38406172
K-Ras(V12) differentially affects the three Akt isoforms in lung and pancreatic carcinoma cells and upregulates E-cadherin and NCAM via Akt3., PMID:38291468
Negative Hyperselection of Resistance Mutations for Panitumumab Maintenance in RAS Wild-Type Metastatic Colorectal Cancer (PanaMa Phase II Trial, AIO KRK 0212)., PMID:38289994
Integrated Molecular Characterization of HER2-Low Breast Cancer Using Next Generation Sequencing (NGS)., PMID:38137385
The anticancer impacts of free and liposomal caffeic acid phenethyl ester (CAPE) on melanoma cell line (A375)., PMID:38111127
Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease., PMID:38075560
DUSP22 suppresses tumor progression by directly dephosphorylating AKT in non-small cell lung cancer., PMID:37937915
Negative hyperselection of elderly patients with RAS and BRAF wild-type metastatic colorectal cancer receiving initial panitumumab plus FOLFOX or 5-FU/LV., PMID:37924647
IL-21/23 axis modulates inflammatory cytokines and RANKL expression in RA CD4+ T cells via p-Akt1 signaling., PMID:37809066
UPLC-Q-TOF-MS, network analysis, and molecular docking to investigate the effect and active ingredients of tea-seed oil against bacterial pathogens., PMID:37745048
Discovery of natural multi-targets neuraminidase inhibitor glycosides compounds against influenza A virus through network pharmacology, virtual screening, molecular dynamics simulation, and in vitro experiment., PMID:37743355
Lycopene alleviates food allergy by modulating the PI3K/AKT pathway in peanut-sensitized BALB/c mice., PMID:37607433
Intravesical BCG in bladder cancer induces innate immune responses against SARS-CoV-2., PMID:37520557
TGF-β1 dominates stromal fibroblast-mediated EMT via the FAP/VCAN axis in bladder cancer cells., PMID:37461061
The MyD88 inhibitor, ST2825, induces cell cycle arrest and apoptosis by suppressing the activation of the NF‑κB/AKT1/p21 pathway in pancreatic cancer., PMID:37326109
Association between the AKT1 single nucleotide polymorphism (rs2498786, rs2494752 and rs5811155) and microscopic polyangiitis risk in a Chinese population., PMID:37029297
IHC expression of AKT in different grades of epithelial dysplasia: An in vitro study., PMID:36588841
Construction of a phos-tag-directed self-assembled fluorescent magnetobiosensor for the simultaneous detection of multiple protein kinases., PMID:36449302
Identification of molecular mechanisms underlying the therapeutic effects of Celosia Cristata on immunoglobulin nephropathy., PMID:36379189
Blockade of the pentraxin 3/CD44 interaction attenuates lung injury-induced fibrosis., PMID:36336784
Exploring the Potential Mechanism of Qi-Shen-Di-Huang Drug Formulary for Myasthenia Gravis (MG) based on UHPLC-QE-MS Network Pharmacology and Molecular Docking Techniques., PMID:36225188
Study on the Potential Mechanism of Semen Strychni against Myasthenia Gravis Based on Network Pharmacology and Molecular Docking with Experimental Verification., PMID:36217431
Systematic elucidation of the bioactive alkaloids and potential mechanism from Sophora flavescens for the treatment of eczema via network pharmacology., PMID:36216196
Tumor microenvironment gene expression profiles associated to complete pathological response and disease progression in resectable NSCLC patients treated with neoadjuvant chemoimmunotherapy., PMID:36171009
Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization., PMID:35931736
Targeting HER3-dependent activation of nuclear AKT improves radiotherapy of non-small cell lung cancer., PMID:35839938
5-HT2B-mediated serotonin activation in enterocytes suppresses colitis-associated cancer initiation and promotes cancer progression., PMID:35664068
Panaxdiol saponins component promotes hematopoiesis by regulating GATA transcription factors of intracellular signaling pathway in mouse bone marrow., PMID:35282082
HERV-W Envelope Triggers Abnormal Dopaminergic Neuron Process through DRD2/PP2A/AKT1/GSK3 for Schizophrenia Risk., PMID:35062349
Dynamics of AQP4 upon exposure to seropositive patient serum before and after Rituximab therapy in Neuromyelitis Optica: A cell-based study., PMID:34715591
Selenium inhibits growth of trastuzumab-resistant human breast cancer cells via downregulation of Akt and beclin-1., PMID:34525121
Defective Reelin/Dab1 signaling pathways associated with disturbed hippocampus development of homozygous yotari mice., PMID:33845123
Active Akt signaling triggers CLL toward Richter transformation via overactivation of Notch1., PMID:33538798
Isoform- and Phosphorylation-specific Multiplexed Quantitative Pharmacodynamics of Drugs Targeting PI3K and MAPK Signaling in Xenograft Models and Clinical Biopsies., PMID:33536190
PI3K/Akt pathway and Nanog maintain cancer stem cells in sarcomas., PMID:33468992
An AKT2-specific nanobody that targets the hydrophobic motif induces cell cycle arrest, autophagy and loss of focal adhesions in MDA-MB-231 cells., PMID:33378961
Inhibition of MEIS3 Generates Cetuximab Resistance through c-Met and Akt., PMID:33376716
The Regenerative Potential of Donkey and Human Milk on the Redox-Sensitive and Proliferative Signaling Pathways of Skin Fibroblasts., PMID:33224432
Determining the effects of trastuzumab, cetuximab and afatinib by phosphoprotein, gene expression and phenotypic analysis in gastric cancer cell lines., PMID:33115415
For research use only. Not for human or drug use.
