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Anti-SDHA Antibody (R3J09)

Catalog #:   RHD92702 Specific References (29) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: ELISA, FCM, IF, WB
Accession: P31040
Overview

Catalog No.

RHD92702

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

SDH2, Flavoprotein subunit of complex II, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, SDHA, SDHF, Fp

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P31040

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3J09

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Raji cells using SDHA mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of THP-1 cells using SDHA mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using SDHA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using SDHA mouse mAb against Jurkat (1), MCF-7 (2), Hela (3), HepG2 (4), PC-3 (5), .HL-60 (6),and NIH/3T3 (7) cell lysate.
References

Identification of a novel transcript of mouse Sdha., PMID:40001192

Melatonin ameliorates Pb-induced mitochondrial homeostasis and ovarian damage through regulating the p38 signaling pathway., PMID:39983510

Comprehensive Genomic Assessment of Advanced-Stage GI Stromal Tumors Using the Japanese National Center for Cancer Genomics and Advanced Therapeutics Database., PMID:39447098

Protein succinylation associated with the progress of hepatocellular carcinoma., PMID:36308411

Evaluating the mitochondrial activity and inflammatory state of dimethyl sulfoxide differentiated PLB-985 cells., PMID:33838400

Detection of Mycobacterium avium Subspecies paratuberculosis (MAP) Microorganisms Using Antigenic MAP Cell Envelope Proteins., PMID:33614761

Identification of Reference Genes in Chicken Intraepithelial Lymphocyte Natural Killer Cells Infected with Very-virulent Infectious Bursal Disease Virus., PMID:32444639

SDHA gain-of-function engages inflammatory mitochondrial retrograde signaling via KEAP1-Nrf2., PMID:31527833

Targeting a Sirt5-Positive Subpopulation Overcomes Multidrug Resistance in Wild-Type Kras Colorectal Carcinomas., PMID:29514096

Identification of antigenic proteins from Mycobacterium avium subspecies paratuberculosis cell envelope by comparative proteomic analysis., PMID:29458660

Mitochondrial reactive oxygen species suppress humoral immune response through reduction of CD19 expression in B cells in mice., PMID:27883180

TLR-2 and TLR-4 transcriptions in unstimulated blood from dogs with leishmaniosis due to Leishmania infantum at the time of diagnosis and during follow-up treatment., PMID:27692322

Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo., PMID:27640183

Identification of DLD, by immunoproteomic analysis and evaluation as a potential vaccine antigen against three Vibrio species in Epinephelus coioides., PMID:26562319

The inflammatory cytokine TWEAK decreases PGC-1α expression and mitochondrial function in acute kidney injury., PMID:26535995

SDHD immunohistochemistry: a new tool to validate SDHx mutations in pheochromocytoma/paraganglioma., PMID:25405498

Adrenocortical oncocytoma presenting as Cushing's syndrome: an additional report of a paediatric case., PMID:25077961

Association of cancer metabolism-related proteins with oral carcinogenesis - indications for chemoprevention and metabolic sensitizing of oral squamous cell carcinoma?, PMID:25048361

Succinate dehydrogenase-deficient renal cell carcinoma: detailed characterization of 11 tumors defining a unique subtype of renal cell carcinoma., PMID:25034258

Cannabinoid control of brain bioenergetics: Exploring the subcellular localization of the CB1 receptor., PMID:24944910

Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer., PMID:24837709

The acetylproteome of Gram-positive model bacterium Bacillus subtilis., PMID:23468065

Immune-related gene expression profile in laboratory common marmosets assessed by an accurate quantitative real-time PCR using selected reference genes., PMID:23451040

Immunohistochemical loss of succinate dehydrogenase subunit A (SDHA) in gastrointestinal stromal tumors (GISTs) signals SDHA germline mutation., PMID:23282968

A NADH dehydrogenase ubiquinone flavoprotein is decreased in patients with dilated cardiomyopathy., PMID:20930427

The cardiac calsequestrin gene (CASQ2) is up-regulated in the thyroid in patients with Graves' ophthalmopathy--support for a role of autoimmunity against calsequestrin as the triggering event., PMID:20039900

Extracellular plasma RNA from colon cancer patients is confined in a vesicle-like structure and is mRNA-enriched., PMID:18456845

Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies., PMID:17346803

Cloning and expression in Escherichia coli of sdhA, the structural gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex., PMID:2987185

Datasheet

Document Download

Anti-SDHA Antibody (R3J09).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-SDHA Antibody (R3J09) [RHD92702]
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