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Anti-CDC27 Antibody (R3H96)

Catalog #:   RHD87702 Specific References (13) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC
Accession: P30260
Overview

Catalog No.

RHD87702

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:200-1:1000

Target

ANAPC3, Cell division cycle protein 27 homolog, CDC27, APC3, Anaphase-promoting complex subunit 3, CDC27Hs, D0S1430E, CDC27 homolog, D17S978E, H-NUC

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P30260

Applications

IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3H96

Data Image
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.
References

Proteomic and serological markers for diagnosing cardia gastric cancer and precursor lesions in a Chinese population., PMID:39455684

The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis., PMID:15615783

Mitotic regulation of the human anaphase-promoting complex by phosphorylation., PMID:14657031

The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis., PMID:12429948

The APC is dispensable for first meiotic anaphase in Xenopus oocytes., PMID:11146630

Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts., PMID:10219083

Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events., PMID:9628895

The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins., PMID:9499405

TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast., PMID:8744951

A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B., PMID:7736580

CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition., PMID:7736578

Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis., PMID:7925276

BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans., PMID:8432735

Datasheet

Document Download

Anti-CDC27 Antibody (R3H96).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-CDC27 Antibody (R3H96) [RHD87702]
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