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Anti-NOS1 Antibody (R3H81)

Catalog #:   RHD84304 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF
Accession: P29475
Overview

Catalog No.

RHD84304

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000

Target

Constitutive NOS, Neuronal NOS, nNOS, N-NOS, bNOS, NOS type I, NC-NOS, Peptidyl-cysteine S-nitrosylase NOS1, NOS1, Nitric oxide synthase, brain

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P29475

Applications

ELISA, FCM, IF

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3H81

Data Image
  • Flow Cytometry
    Flow cytometric analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
References

N2-Polarized Neutrophils Reduce Inflammation in Rosacea by Regulating Vascular Factors and Proliferation of CD4+ T Cells., PMID:34953863

An advanced method for the immunohistochemical detection of nitric oxide synthase (NOS) in the female genital tract., PMID:34116059

Structural and functional relations between the connective tissue and epithelium of enamel organ and their role during enamel maturation., PMID:34100179

Re-evaluation of the immunohistochemical distribution of isoforms of nitric oxide synthase in the human prostate: A light and electron microscopical study., PMID:34051107

Hypoxia-induced expression of neuronal nitric oxide synthase in astrocytes of human corpus callosum., PMID:33709161

Blood pressure and albuminuria in a female mouse model of systemic lupus erythematosus: impact of long-term high salt consumption., PMID:32813539

Effects of Edaravone on Nitric Oxide, Hydroxyl Radicals and Neuronal Nitric Oxide Synthase During Cerebral Ischemia and Reperfusion in Mice., PMID:31882337

Arginase enzymes in the human prostate: A molecular biological and immunohistochemical approach., PMID:31482616

Bisphenol-A exposure induced neurotoxicity in glutamatergic neurons derived from human embryonic stem cells., PMID:30953815

Effects of TNF-α on penile structure alteration in rats with hyperprolactinemia., PMID:28763467

Osteopontin inhibits osteoblast responsiveness through the down-regulation of focal adhesion kinase mediated by the induction of low-molecular weight protein tyrosine phosphatase., PMID:28331074

Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus., PMID:28028361

Defining Subpopulations of Arcuate Nucleus GABA Neurons in Male, Female, and Prenatally Androgenized Female Mice., PMID:27710963

[Effect of adaptation to hypoxia on expression of NO synthase isoforms in rat myocardium]., PMID:27116881

Insights into the C-terminal Peptide Binding Specificity of the PDZ Domain of Neuronal Nitric-oxide Synthase: CHARACTERIZATION OF THE INTERACTION WITH THE TIGHT JUNCTION PROTEIN CLAUDIN-3., PMID:27030110

Identification of a novel splice variant of neuronal nitric oxide synthase, nNOSβ, in myofilament fraction of murine cardiomyocytes., PMID:26271450

PnPP-19, a Synthetic and Nontoxic Peptide Designed from a Phoneutria nigriventer Toxin, Potentiates Erectile Function via NO/cGMP., PMID:26119670

Colocalization of cannabinoid receptor 1 with somatostatin and neuronal nitric oxide synthase in rat brain hippocampus., PMID:26115586

Synthetic and endogenous cannabinoids protect retinal neurons from AMPA excitotoxicity in vivo, via activation of CB1 receptors: Involvement of PI3K/Akt and MEK/ERK signaling pathways., PMID:25989217

Expression of nNOS in the human larynx., PMID:25656267

Inhibition of i-NOS but not n-NOS protects rat primary cell cultures against MPP(+)-induced neuronal toxicity., PMID:25503828

Blockade of PD1 and TIM3 restores innate and adaptive immunity in patients with acute alcoholic hepatitis., PMID:25479137

Intracallosal neuronal nitric oxide synthase neurons colocalize with neurokinin 1 substance P receptor in the rat., PMID:25312245

Global analysis of S-nitrosylation sites in the wild type (APP) transgenic mouse brain-clues for synaptic pathology., PMID:24895380

Aerobic exercise inhibits sympathetic nerve sprouting and restores β-adrenergic receptor balance in rats with myocardial infarction., PMID:24842290

Myogenin, AP2β, NOS-1, and HMGA2 are surrogate markers of fusion status in rhabdomyosarcoma: a report from the soft tissue sarcoma committee of the children's oncology group., PMID:24618610

Neuro-anatomical evidence indicating indirect modulation of macrophages by vagal efferents in the intestine but not in the spleen., PMID:24489965

Birthdating of myenteric neuron subtypes in the small intestine of the mouse., PMID:23861145

Bone and muscle structure and quality preserved by active versus passive muscle exercise on a new stepper device in 21 days tail-suspended rats., PMID:23728103

Nerve injury-induced protein 1 (Ninjurin-1) is a novel therapeutic target for cavernous nerve injury-induced erectile dysfunction in mice., PMID:23551591

Myenteric neuron numbers are maintained in aging mouse distal colon., PMID:23517051

Neural nitric oxide synthase participates in pemphigus vulgaris acantholysis through upregulation of Rous sarcoma, mammalian target of rapamycin and focal adhesion kinase., PMID:23362871

Effect of mesenchymal stem cells associated to matrixen on the erectile function in the rat model with bilateral cavernous nerve crushing injury., PMID:23302404

Pasireotide (SOM230) protects the retina in animal models of ischemia induced retinopathies., PMID:22960304

Fatigue and muscle atrophy in a mouse model of myasthenia gravis is paralleled by loss of sarcolemmal nNOS., PMID:22952904

A perioculomotor nitridergic population in the macaque and cat., PMID:22836763

Galanin expression in the mouse major pelvic ganglia during explant culture and following cavernous nerve transection., PMID:22585545

AAT polymorphisms in intron 20 of NOS1 confer vulnerability to mite-induced allergic rhinitis in Chinese patients., PMID:22448456

Expression of nitric oxide synthases in leukocytes in nasal polyps., PMID:22374200

Stimulation of unprimed macrophages with immune complexes triggers a low output of nitric oxide by calcium-dependent neuronal nitric-oxide synthase., PMID:22205701

nNOS deficiency-induced cell proliferation depletes the neurogenic reserve., PMID:22027181

The beta-isoform of neuronal nitric oxide synthase (nNOS) lacking the PDZ domain is localized at the sarcolemma., PMID:21945318

Nitration of tryptophan in ribosomal proteins is a novel post-translational modification of differentiated and naïve PC12 cells., PMID:21642007

TRPV1 expression in acupuncture points: response to electroacupuncture stimulation., PMID:21256210

Astrocytes express N-methyl-D-aspartate receptor subunits in development, ischemia and post-ischemia., PMID:21116713

Neuronal nitric oxide synthase supports Renin release during sodium restriction through inhibition of phosphodiesterase 3., PMID:20651700

The effects of NOS1 gene on asthma and total IgE levels in Taiwanese children, and the interactions with environmental factors., PMID:20609134

Spinal motoneuron synaptic plasticity after axotomy in the absence of inducible nitric oxide synthase., PMID:20497552

Short-chain fatty acids regulate the enteric neurons and control gastrointestinal motility in rats., PMID:20152836

Effect of cryopreservation on nitric oxide production by stallion spermatozoa., PMID:19605785

Datasheet

Document Download

Anti-NOS1 Antibody (R3H81).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-NOS1 Antibody (R3H81) [RHD84304]
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