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Anti-RAB5A Antibody (R3F69)

Catalog #:   RHD39303 Specific References (40) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P20339
Overview

Catalog No.

RHD39303

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Ras-related protein Rab-5A, RAB5A, RAB5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P20339

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3F69

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using Rab5a mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using Rab5a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Rab5a mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human duodenum tissues using Rab5a mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using Rab5a mouse mAb against Hela (1) and K562 (2) cell lysate.
  • Western blot
    Western blot analysis using Rab5a mouse mAb against Hela (1) and K562 (2) cell lysate.
References

CTRP7 as a molecular biomarker associating with responsiveness to pulmonary vasodilators: insights from human and animal studies in pulmonary arterial hypertension., PMID:40504501

The mechanisms of HER2 targeted ADCs are dependent on Rab GTPases., PMID:40297622

A trafficking regulatory subnetwork governs αVβ6 integrin-HER2 cross-talk to control breast cancer invasion and drug resistance., PMID:39630893

CD2AP is Co-Expressed with Tropomyosin-Related Kinase A and Ras-Related Protein Rab-5A in Cholinergic Neurons of the Murine Basal Forebrain., PMID:39211110

The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles., PMID:35847504

RAB5A expression is a predictive biomarker for trastuzumab emtansine in breast cancer., PMID:34741021

Direct interaction between Rab5a and Rab4a enhanced epidermal growth factor-stimulated proliferation of gastric cancer cells., PMID:34721780

Epitopes for Multivalent Vaccines Against Listeria, Mycobacterium and Streptococcus spp: A Novel Role for Glyceraldehyde-3-Phosphate Dehydrogenase., PMID:33194812

Programmed death-ligand 1 monoclonal antibody-linked immunoliposomes for synergistic efficacy of miR-130a and oxaliplatin in gastric cancers., PMID:31290727

Association of an IRF3 putative functional uORF variant with resistance to Brucella infection: A candidate gene based analysis of InDel polymorphisms in goats., PMID:30477986

Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase., PMID:29127256

Modifiers of prion protein biogenesis and recycling identified by a highly parallel endocytosis kinetics assay., PMID:28341739

Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells., PMID:27110488

The Rab5A gene of marine fish, large yellow croaker (Larimichthys crocea), and its response to the infection of Cryptocaryon irritans., PMID:27108380

Rab5a is required for spindle length control and kinetochore-microtubule attachment during meiosis in oocytes., PMID:24876181

Identification of an immune-regulated phagosomal Rab cascade in macrophages., PMID:24569883

Endocytosis of HERG is clathrin-independent and involves arf6., PMID:24392021

Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages., PMID:24373249

Internalization of adhesion junction proteins and their association with recycling endosome marker proteins in rat seminiferous epithelium., PMID:22157319

Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway., PMID:21849022

Segmental and subcellular distribution of CFTR in the kidney., PMID:21594792

A structural basis for Lowe syndrome caused by mutations in the Rab-binding domain of OCRL1., PMID:21378754

RNA interference and single particle tracking analysis of hepatitis C virus endocytosis., PMID:20041214

Endothelin-converting enzyme-1 degrades internalized somatostatin-14., PMID:18276747

Phagocytosis-induced apoptosis of macrophages is linked to uptake, killing and degradation of bacteria., PMID:18085665

Agonist-induced endocytosis of rat somatostatin receptor 1., PMID:17170097

Expression and subcellular localization of Spred proteins in mouse and human tissues., PMID:15580519

Multiple regions contribute to membrane targeting of Rab GTPases., PMID:15561774

The role of alpha(5)beta(1)-integrin in the IGF-I-induced migration of extravillous trophoblast cells during the process of implantation., PMID:14742693

Apoptosis by Cd2+ or CdMT in proximal tubule cells: different uptake routes and permissive role of endo/lysosomal CdMT uptake., PMID:12890648

Co-localization of rab4 with endocytosis-related proteins in the rat parotid glands., PMID:12703553

Agonist-induced endocytosis of lysophosphatidic acid-coupled LPA1/EDG-2 receptors via a dynamin2- and Rab5-dependent pathway., PMID:12668728

Dynamin and Rab5a-dependent trafficking and signaling of the neurokinin 1 receptor., PMID:11306580

Colocalization of Fc gamma RI-targeted antigen with class I MHC: implications for antigen processing., PMID:11160307

Direct interaction of EEA1 with Rab5b., PMID:10491193

[Transcytosis of immunoglobulin IgG]., PMID:9990196

Identification of Rab3-, Rab5a- and synaptobrevin II-like proteins in a preparation of rat kidney vesicles containing the vasopressin-regulated water channel., PMID:7540151

Mapping of Ras-related GTP-binding proteins by GTP overlay following two-dimensional gel electrophoresis., PMID:8058727

The involvement of the small GTP-binding protein Rab5a in neuronal endocytosis., PMID:8043272

Rab5a is a common component of the apical and basolateral endocytic machinery in polarized epithelial cells., PMID:8197185

Datasheet

Document Download

Anti-RAB5A Antibody (R3F69).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-RAB5A Antibody (R3F69) [RHD39303]
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