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Anti-CHRM3 Antibody (R3F63)

Catalog #:   RHD38803 Specific References (13) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: FCM, IF, IHC
Accession: P20309
Overview

Catalog No.

RHD38803

Species reactivity

Human, Mouse, Rabbit

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:25-1:50, IHC: 1:200-1:400

Target

CHRM3, Muscarinic acetylcholine receptor M3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P20309

Applications

FCM, IF, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3F63

Data Image
  • Flow Cytometry
    Flow cytometric analysis of SK-BR-3 cells using CHRM3 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of SK-N-SH cells using CHRM3 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Confocal Immunofluorescence analysis of NIH3T3 cells using CHRM3 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CHRM3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using CHRM3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using CHRM3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded mouse liver tissues using CHRM3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded rabbit liver tissues using CHRM3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded rabbit kidney tissues using CHRM3 mouse mAb with DAB staining.
References

Epitomic profiling and functional characteristics of pemphigus vulgaris autoantibody binding to keratinocyte M3 muscarinic acetylcholine receptor., PMID:40120680

Severity of neurological Long-COVID symptoms correlates with increased level of autoantibodies targeting vasoregulatory and autonomic nervous system receptors., PMID:37689093

Antibodies to the Muscarinic Acetylcholine Receptor M3 in Primary Biliary Cholangitis Inhibit Receptor Function on Cholangiocytes., PMID:32695096

M3 muscarinic acetylcholine receptor-reactive Th17 cells in primary Sjögren's syndrome., PMID:32614803

RNA sequencing profiles and diagnostic signatures linked with response to ramucirumab in gastric cancer., PMID:32060041

Autoantibodies against muscarinic acetylcholine receptor M3 in Sjogren's syndrome and corresponding mouse models., PMID:29772545

Differential expression of M3 muscarinic receptors in progressive colon neoplasia and metastasis., PMID:28416748

New Etiology of Cholinergic Urticaria., PMID:27584968

Urothelial Barrier Deficits, Suburothelial Inflammation and Altered Sensory Protein Expression in Detrusor Underactivity., PMID:27436428

Type 3 Muscarinic Receptors Contribute to Clearance of Citrobacter rodentium., PMID:25985244

Suitability of Nicotinic Acetylcholine Receptor α7 and Muscarinic Acetylcholine Receptor 3 Antibodies for Immune Detection: Evaluation in Murine Skin., PMID:25673288

Pemphigus vulgaris autoantibody profiling by proteomic technique., PMID:23505434

Muscarinic receptor agonists stimulate human colon cancer cell migration and invasion., PMID:21273532

Datasheet

Document Download

Anti-CHRM3 Antibody (R3F63).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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For research use only. Not for human or drug use.

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Anti-CHRM3 Antibody (R3F63) [RHD38803]
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