Anti-NCK1 Antibody (R3E45)

Catalog No.

RHD16602

Species reactivity

Human, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

SH2/SH3 adaptor protein NCK-alpha, Nck-1, NCK adaptor protein 1, NCK, NCK1, Cytoplasmic protein NCK1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P16333

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3E45

Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion., PMID:31638742

A Study of Interleukin-2 Production and Nck Adaptor Molecule Gene Expression in Systemic Lupus Erythematosus Patients., PMID:29911835

Nck influences preosteoblastic/osteoblastic migration and bone mass., PMID:26621720

Evidence for inducible recruitment of Wiskott-Aldrich syndrome protein to T cell receptor-CD3 complex in Jurkat T cells., PMID:26342115

Depletion of the adaptor protein NCK increases UV-induced p53 phosphorylation and promotes apoptosis., PMID:24086708

The adaptor molecule Nck localizes the WAVE complex to promote actin polymerization during CEACAM3-mediated phagocytosis of bacteria., PMID:22448228

Essential role of the adaptor protein Nck1 in Jurkat T cell activation and function., PMID:22132889

Mapping protein interactions by combining antibody affinity maturation and mass spectrometry., PMID:21704603

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display., PMID:20164216

Nck adaptor proteins link Tks5 to invadopodia actin regulation and ECM degradation., PMID:19596797

The XLP syndrome protein SAP interacts with SH3 proteins to regulate T cell signaling and proliferation., PMID:18951976

Rapid WAVE dynamics in dendritic spines of cultured hippocampal neurons is mediated by actin polymerization., PMID:16190876

Deleted in colorectal cancer binding netrin-1 mediates cell substrate adhesion and recruits Cdc42, Rac1, Pak1, and N-WASP into an intracellular signaling complex that promotes growth cone expansion., PMID:15788770

Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor protein with the TrkB tyrosine kinase receptor., PMID:12074588

Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways., PMID:9843575

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Anti-NCK1 Antibody (R3E45).pdf