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Anti-CD43/SPN/Leukosialin Antibody (R3E34)

Catalog #:   RHD15501 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IHC, WB
Accession: P16150
Overview

Catalog No.

RHD15501

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

GPL115, Leukocyte sialoglycoprotein, Leukosialin, Sialophorin, SPN, Galactoglycoprotein, CD43, CD43ct, CD43-ct, GALGP

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P16150

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3E34

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using CD43 mouse mAb (green) and negative control (red).
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD43 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using CD43 mouse mAb against HEK293-6E (1), HEK293 (2),K562 (3), MOLT4 (4), Jurkat (5),and PANC-1 (6) cell lysate.
References

Deep profiling of B cells responding to various pathogens uncovers compartments in IgG memory B cell and antibody-secreting lineages., PMID:39970201

Integration of CD200, CD43 and ROR1 in Multiparameter Flow Cytometry (MFC) Routine Panels for the Differential Diagnosis of B-cell lymphoproliferative Disorders (B-LPDs)., PMID:39830795

Peritoneal B1 and B2 cells respond differently to LPS and IL-21 stimulation., PMID:38615627

Faster clinical decisions in B-cell acute lymphoblastic leukaemia: A single flow cytometric 12-colour tube improves diagnosis and minimal residual disease follow-up., PMID:38432068

Improvement of Severe Colon Stricture after Rituximab Therapy for Concomitant Mucosa-associated Lymphoid Tissue Lymphoma in a Patient with Ulcerative Colitis., PMID:37997222

Identification of novel targets for immunotherapies in subsets of patients with diffuse large B-cell lymphoma (DLBCL)., PMID:37964473

A Push to Consider Mantle Cell Lymphoma in Adults with Leukemia/Lymphoma with Blastoid Morphology., PMID:37873796

Phospholipase D4 as a signature of toll-like receptor 7 or 9 signaling is expressed on blastic T-bet + B cells in systemic lupus erythematosus., PMID:37840148

Shared and unique antibody and B cell profiles in HIV-positive and HIV-negative individuals with cryptococcal meningoencephalitis., PMID:37771088

Transplantation elicits a clonally diverse CD8+ T cell response that is comprised of potent CD43+ effectors., PMID:37590141

Plasma cells are not restricted to the CD27+ phenotype: characterization of CD27-CD43+ antibody-secreting cells., PMID:37492569

Tumor cell-intrinsic and tumor microenvironmental conditions co-determine signaling by the glycoimmune checkpoint receptor Siglec-7., PMID:37253806

CD169-CD43 interaction is involved in erythroblastic island formation and erythroid differentiation., PMID:36861412

B cell receptor-induced IL-10 production from neonatal mouse CD19+CD43- cells depends on STAT5-mediated IL-6 secretion., PMID:36735294

Bone marrow-derived naïve B lymphocytes improve heart function after myocardial infarction: a novel cardioprotective mechanism for empagliflozin., PMID:36171393

T-helper cell regulation of CD45 phosphatase activity by galectin-1 and CD43 governs chronic lymphocytic leukaemia proliferation., PMID:35655388

Plasma Cell-Predominant Lichen Planopilaris., PMID:35076427

Hematogones with light chain restriction: A potential diagnostic pitfall when using flow cytometry analysis to assess bone marrow specimens., PMID:34735934

A new score including CD43 and CD180: Increased diagnostic value for atypical chronic lymphocytic leukemia., PMID:34061467

Immunoglobulins and Transcription Factors in Otitis Media., PMID:33801155

Quick and easy purification of murine untouched naive B cells or germinal center B cells by MACS., PMID:33718892

Chemokine Receptor-6 Promotes B-1 Cell Trafficking to Perivascular Adipose Tissue, Local IgM Production and Atheroprotection., PMID:33679793

Interferon α Enhances B Cell Activation Associated With FOXM1 Induction: Potential Novel Therapeutic Strategy for Targeting the Plasmablasts of Systemic Lupus Erythematosus., PMID:33633721

Therapeutic afucosylated monoclonal antibody and bispecific T-cell engagers for T-cell acute lymphoblastic leukemia., PMID:33597219

Melanoma cells can be eliminated by sialylated CD43 × CD3 bispecific T cell engager formats in vitro and in vivo., PMID:33225419

[Assessment of the regenerative potential of platelet-rich plasma in urethroplasty in the experimental model]., PMID:32897011

A novel transgenic mouse strain expressing PKCβII demonstrates expansion of B1 and marginal zone B cell populations., PMID:32753714

Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes., PMID:32582197

Contribution of immunophenotype to the investigation and differential diagnosis of Burkitt lymphoma, double-hit high-grade B-cell lymphoma, and single-hit MYC-rearranged diffuse large B-cell lymphoma., PMID:32497402

Significance of CD10 protein expression in the diagnostics of follicular lymphoma: A comparison of conventional immunohistochemistry with flow cytometry supported by the establishment of BCL2 and BCL6 rearrangements., PMID:32364682

Suppressed humoral immunity is associated with dengue nonstructural protein NS1-elicited anti-death receptor antibody fractions in mice., PMID:32286343

Determination of CD Markers Profile of the Cell Line Infected by S15 Vaccine Strain of Theileria annulata Schizont Using RT-PCR Analysis., PMID:31939260

Primary Low-Grade B-Cell Lymphoma of Skull With Translocation Between Immunoglobulin and Interferon Regulatory Factor 4 Genes., PMID:31631721

Encoding the Sequence of Specific Autoantibodies Against beta-Amyloid and alpha-Synuclein in Neurodegenerative Diseases., PMID:31507618

Human B1 Cells are the Main Blood Group A-Specific B Cells That Have a Moderate Correlation With Anti-A Antibody Titer., PMID:31432639

JL1 Antigen Expression on Bone Marrow Lymphoma Cells from Patients With Non-Hodgkin Lymphoma., PMID:31432632

Pediatric Mesenchymal Stem Cells Exhibit Immunomodulatory Properties Toward Allogeneic T and B Cells Under Inflammatory Conditions., PMID:31245368

Immunophenotyping of Murine Precursor B-Cell Leukemia/Lymphoma: A Comparison of Immunohistochemistry and Flow Cytometry., PMID:31170889

Responsiveness of chronic lymphocytic leukemia cells to B-cell receptor stimulation is associated with low expression of regulatory molecules of the nuclear factor-κB pathway., PMID:31097630

A Chemo-enzymatically Linked Bispecific Antibody Retargets T Cells to a Sialylated Epitope on CD43 in Acute Myeloid Leukemia., PMID:31064847

Human B-1 Cells and B-1 Cell Antibodies Change With Advancing Age., PMID:30941130

Glycosylation in health and disease., PMID:30858582

The Incidence and Immunophenotypic and Genetic Features of JL1 Expressing Cells and the Therapeutic Potential of an Anti-JL1 Antibody in De Novo Pediatric Acute Leukemias., PMID:30809981

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector., PMID:30582589

Differential inhibition of mucin-type O-glycosylation (MTOG) induced by peracetyl N-thioglycolyl-d-galactosamine (Ac5GalNTGc) in myeloid cells., PMID:30336974

Sinomenine Inhibits the Progression of Rheumatoid Arthritis by Regulating the Secretion of Inflammatory Cytokines and Monocyte/Macrophage Subsets., PMID:30319663

B cells from patients with multiple sclerosis have a pathogenic phenotype and increased LTα and TGFβ1 response., PMID:30244922

Identification of Human B-1 Helper T Cells With a Th1-Like Memory Phenotype and High Integrin CD49d Expression., PMID:30061889

Potential killers exposed: tracking endogenous influenza-specific CD8+ T cells., PMID:29972699

Early Generated B-1-Derived B Cells Have the Capacity To Progress To Become Mantle Cell Lymphoma-like Neoplasia in Aged Mice., PMID:29898964

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-CD43/SPN/Leukosialin Antibody (R3E34) [RHD15501]
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