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Anti-CD45/PTPRC Antibody (R3B04)

Catalog #:   RHC34007 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P08575
Overview

Catalog No.

RHC34007

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08575

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3B04

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using CD45R mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using CD45R mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using CD45R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human tonsil tissues using CD45R mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CD45R mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CD45R mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using CD45R mouse mAb against Jurkat (1), Rat Serum (2), and mouse Serum (3) cell lysate.
References

Flow Cytometric Assessment of TRBC1 Expression for the Diagnosis of T-Cell Lymphoma: Clinical Utility and Pitfalls Related to T-Cell Clones of Uncertain Significance., PMID:40521694

HLA-DQ monomer-based enrichment of alloreactive CD4 T cells., PMID:40502077

Detectable SARS-CoV-2 specific immune responses in recovered unvaccinated individuals 250 days post wild type infection., PMID:40498720

Phenotypic Shifts in Macrophages Within Advanced Atherosclerotic Plaques in Humans., PMID:40496346

Single-cell proteomic analysis reveals Multiple Myeloma heterogeneity and the dynamics of the tumor immune microenvironment in precursor and advanced states., PMID:40482292

Site-Specific Immune and Stromal Architecture Drive Resistance to Trastuzumab Deruxtecan in HER2+ Metastatic Breast Cancer., PMID:40475468

The cytotoxic effect of quercetin-induced apoptosis on lung metastatic cells from giant cell tumor of bone., PMID:40418557

FACS-Proteomics strategy toward extracellular vesicles single-phenotype characterization in biological fluids: exploring the role of leukocyte-derived EVs in multiple sclerosis., PMID:40394633

Alterations in CD8+CD45RA+CCR7- T cells as a potential biomarker for primary Sjögren's syndrome., PMID:40388849

Postnatal epigenetic differences in calves following transient fetal infection with bovine viral diarrhea virus., PMID:40316897

Multiplex immunophenotyping of human acute myeloid leukemia patients revealed single -cell heterogeneity with special attention on therapy sensitive and therapy resistant subpopulations., PMID:40313947

TRPV1 Defends the Healthy Murine Cornea against Staphylococcus aureus Adhesion Independently of Sensory Nerve Firing., PMID:40291673

Fetal-neonatal alloimmune thrombocytopenia: Mothers are affected too., PMID:40275830

Human Leukocyte Antigen Haplotypes Predisposing to Celiac Disease in Patients With Endometriosis., PMID:40260879

Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF., PMID:40258831

Evaluation of a cost-effective gating strategy for CD4+ T lymphocyte enumeration in HIV-infected individuals via flow cytometry., PMID:40253874

Morphological characteristics, extracellular vesicle structure and stem-like specificity of human follicular fluid cell subpopulation during osteodifferentiation., PMID:40253818

The bone marrow niche and hematopoietic system are distinctly remodeled by CD45-targeted astatine-211 radioimmunotherapy., PMID:40236126

Rare Subset of T Cells Form Heterotypic Clusters with Circulating Tumor Cells to Foster Cancer Metastasis., PMID:40236049

Antigen quantity is responsible for the discrepancy between phenotype and single antigen beads for the detection of human leukocyte antigen DQ antibody: Potential clinical implications., PMID:40228791

Recognition of different subsets of alloreactive T cells by activation-induced markers., PMID:40204006

Old hematopoietic stem cells retain competence to reconstitute a youthful B cell system that is highly responsive to protein-based vaccination., PMID:40188072

High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype., PMID:40158231

Potential role of liver-resident CD3+ macrophages in HBV clearance in a mouse hepatitis B model., PMID:40143948

The influence of genetic factors on the clinical manifestations and response to systemic treatment of plaque psoriasis., PMID:40095058

4-Color Flow Cytometric Cytotoxicity Crossmatch Using Whole Blood Lysis., PMID:40090806

Combination therapy with Chicoric acid and PD-1/PD-L1 blockade improves the immunotherapy response in patient-derived ovarian cancer xenograft model., PMID:40087780

Leveraging T cell co-stimulation for enhanced therapeutic efficacy of trispecific antibodies targeting prostate cancer., PMID:40081942

In vivo antibody labeling route and fluorophore dictate labeling efficiency, sensitivity, and longevity., PMID:40073090

Migration of Regulatory T Cells to the Peritumor Microenvironment of Experimental Glioblastoma., PMID:40071070

Phase 1 study of IMCnyeso, a T cell receptor bispecific ImmTAC targeting NY-ESO-1-expressing malignancies., PMID:40054461

Early CTLA4 increase in CD45+ blood cells: an emerging biomarker of atezolizumab-bevacizumab resistance and worse survival in advanced hepatocarcinoma., PMID:40048814

Analysis of Germinal Center Reaction in Competitive Bone Marrow Chimeric Rag2-/-γc-/-Mice., PMID:40029512

Mapping the nanoscale organization of the human cell surface proteome reveals new functional associations and surface antigen clusters., PMID:40027624

Nucleated Red Blood Cells Secrete Haptoglobin to Induce Immunosuppressive Function in Monocytes., PMID:40017804

Effect of chemoradiotherapy on the dynamics of circulating lymphocyte subsets in patients with non-metastatic nasopharyngeal carcinoma., PMID:40012553

Bioorthogonal Chemical Engineering of rAAV Capsid: Advancing Gene Therapy Targeting Using Proteins., PMID:39992873

Characterization of the Cellular Immune Response to Group B Streptococcal Vaginal Colonization., PMID:39975125

Spatially Resolved, Multiregion Proteomics for Prediction of Immunotherapy Outcome in Deficient Mismatch Repair Metastatic Colorectal Cancer., PMID:39969975

Allogeneic Chimeric Antigen Receptor T-Cell Products Cemacabtagene Ansegedleucel/ALLO-501 in Relapsed/Refractory Large B-Cell Lymphoma: Phase I Experience From the ALPHA2/ALPHA Clinical Studies., PMID:39946666

Immunohistochemical identification of immune cell subsets in formalin- and zinc-fixed, paraffin-embedded tissues from chicken and duck using commercial antibodies., PMID:39938274

Plasma leukocyte-derived extracellular vesicles are related to target vessel revascularization in off-pump coronary artery bypass grafting., PMID:39897018

Trends in the Perioperative Practices for Immunological Assessment and Immunosuppression Strategies for Patients Undergoing Intestinal Transplantation at American Transplant Centers., PMID:39890513

Combining magnetically isolated CD45 cells with serum maintains intact drug responsiveness for ELISpot analysis in clinical trials., PMID:39882960

Upregulation of interferon-γ response genes in monocytes and T cells identified by single-cell transcriptomics in patients with anti-citrullinated peptide antibody-positive early rheumatoid arthritis., PMID:39877346

Clinico-Pathological Significance of Tumor Infiltrating Immune Cells in Oral Squamous Cell Carcinoma-Hope or Hype?, PMID:39865357

Metabolic reprogramming and macrophage expansion define ACPA-negative rheumatoid arthritis: insights from single-cell RNA sequencing., PMID:39830504

Dynamic analysis of intrahepatic T cells reveals a unique group of restorative Cxcr3+ tissue-resident CD4 T cells in acute liver injury., PMID:39828240

Targeting CD45 by gene-edited CAR T cells for leukemia eradication and hematopoietic stem cell transplantation preconditioning., PMID:39822756

Protocols for circulating neutrophil depletion in neonatal C57Bl/6 mice., PMID:39821323

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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For research use only. Not for human or drug use.

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Anti-CD45/PTPRC Antibody (R3B04) [RHC34007]
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