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Anti-PDHA1 Antibody (R3A91)

Catalog #:   RHC33502 Specific References (10) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, FCM, WB
Accession: P08559
Overview

Catalog No.

RHC33502

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, WB: 1:500-1:2000

Target

PHE1A, PDHE1-A type I, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHA1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08559

Applications

ELISA, FCM, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3A91

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using PDHA1 mouse mAb (green) and negative control (red).
  • Western blot
    Western blot analysis using PDHA1 mouse mAb against HepG2 (1), HL-60 (2),SK-OV-3 (3),PC-3 (4) and PANC-1 (5) cell lysate.
References

Glaucoma-Associated CDR1 Peptide Promotes RGC Survival in Retinal Explants through Molecular Interaction with Acidic Leucine Rich Nuclear Phosphoprotein 32A (ANP32A)., PMID:37509196

Effects of UBE3A on Cell and Liver Metabolism through the Ubiquitination of PDHA1 and ACAT1., PMID:36920305

Anti-PDHA1 antibody is detected in a subset of patients with schizophrenia., PMID:32404964

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance., PMID:30786811

Identification of immunogenic proteins and evaluation of recombinant PDHA1 and GAPDH as potential vaccine candidates against Streptococcus iniae infection in flounder (Paralichthys olivaceus)., PMID:29847601

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins., PMID:29786648

Pyruvate dehydrogenase complex and lactate dehydrogenase are targets for therapy of acute liver failure., PMID:29580866

SIRT3 deacetylates and increases pyruvate dehydrogenase activity in cancer cells., PMID:25152236

Increased 8-hydroxy-2'-deoxyguanosine in plasma and decreased mRNA expression of human 8-oxoguanine DNA glycosylase 1, anti-oxidant enzymes, mitochondrial biogenesis-related proteins and glycolytic enzymes in leucocytes in patients with systemic lupus erythematosus., PMID:24345202

Pemphigus vulgaris autoantibody profiling by proteomic technique., PMID:23505434

Datasheet

Document Download

Anti-PDHA1 Antibody (R3A91).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-PDHA1 Antibody (R3A91) [RHC33502]
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