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Anti-TUBB Antibody (R3A25)

Catalog #:   RHC26001 Specific References (25) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P07437
Overview

Catalog No.

RHC26001

Species reactivity

Human, Mouse, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:1000-1:2000

Target

Tubulin beta-5 chain, TUBB, Tubulin beta chain, TUBB5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P07437

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3A25

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using TUBB1 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of HepG2 cells using TUBB1 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of HL-60 cells using TUBB1 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using TUBB1 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of THP-1 cells using TUBB1 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of THP-1 cells using TUBB1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using TUBB1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using TUBB1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using TUBB1 mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), and COS-7 (7) cell lysate.
References

Luminex-based pilot study identifies novel serum autoantibodies associated with disease activity in patients with lupus nephritis., PMID:40500568

Identification of antigenic epitopes recognized by tumor infiltrating lymphocytes in high grade serous ovarian cancer by multi-omics profiling of the auto-antigen repertoire., PMID:36943460

The Pre-Transplant Non-HLA Antibody Burden Associates With the Development of Histology of Antibody-Mediated Rejection After Kidney Transplantation., PMID:35250981

Single-dose administration and the influence of the timing of the booster dose on immunogenicity and efficacy of ChAdOx1 nCoV-19 (AZD1222) vaccine: a pooled analysis of four randomised trials., PMID:33617777

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545

Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease., PMID:31462148

Discovery of a Novel Microtubule Targeting Agent as an Adjuvant for Cancer Immunotherapy., PMID:30406141

Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy., PMID:27424607

A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67., PMID:27246531

Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67., PMID:26226545

[Screening of citrullinated proteins in ten tumor cell lines]., PMID:25975782

Differential S-nitrosylation of proteins in Alzheimer's disease., PMID:24157928

Investigating citrullinated proteins in tumour cell lines., PMID:24099319

Effect of all-trans retinoic acid on the differentiation of U87 glioma stem/progenitor cells., PMID:23852377

Screening citrullinated proteins in synovial tissues of rheumatoid arthritis using 2-dimensional western blotting., PMID:23242181

Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer., PMID:21045152

Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells., PMID:16206250

Selection with a regulated cell growth switch increases the likelihood of expression for a linked gamma-globin gene., PMID:15885608

Testis fascin (FSCN3): a novel paralog of the actin-bundling protein fascin expressed specifically in the elongate spermatid head., PMID:11925108

In vivo blockade of CTLA-4 enhances the priming of responsive T cells but fails to prevent the induction of tumor antigen-specific tolerance., PMID:10500201

Conversion of tumor-specific CD4+ T-cell tolerance to T-cell priming through in vivo ligation of CD40., PMID:10395323

Correction of hemophilia B in canine and murine models using recombinant adeno-associated viral vectors., PMID:9883841

beta-Catenin associates with the actin-bundling protein fascin in a noncadherin complex., PMID:8794867

Strain related variations in adenovirally mediated transgene expression from mouse hepatocytes in vivo: comparisons between immunocompetent and immunodeficient inbred strains., PMID:7719932

Datasheet

Document Download

Anti-TUBB Antibody (R3A25).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-TUBB Antibody (R3A25) [RHC26001]
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