Catalog No.
RHC20904
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
Eukaryotic translation initiation factor 4E, EIF4F, mRNA cap-binding protein, eIF4E, eIF-4E, eIF-4F 25 kDa subunit, EIF4EL1, EIF4E
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P06730
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R2Z83
Downregulation of PD-L1 expression by Wnt pathway inhibition to enhance PD-1 blockade efficacy in hepatocellular carcinoma., PMID:40211365
Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway., PMID:39937170
MOGAT3-mediated DAG accumulation drives acquired resistance to anti-BRAF/anti-EGFR therapy in BRAFV600E-mutant metastatic colorectal cancer., PMID:39436710
Autoantibody profiling of patients with immune checkpoint inhibitor-associated myocarditis: a pilot study., PMID:39324142
Chemical and topological design of multicapped mRNA and capped circular RNA to augment translation., PMID:39313647
Tirbanibulin decreases cell proliferation and downregulates protein expression of oncogenic pathways in human papillomavirus containing HeLa cells., PMID:38967656
Phospho-eIF4E stimulation regulates coronavirus entry by selective expression of cell membrane-residential factors., PMID:38299843
Enhanced Antipediatric Sarcoma Effect of Everolimus with Secukinumab by Targeting IL17A., PMID:38295302
An AlphaFold2 map of the 53BP1 pathway identifies a direct SHLD3-RIF1 interaction critical for shieldin activity., PMID:37306046
A Totipotent "All-In-One" Peptide Sequentially Blocks Immune Checkpoint and Reverses the Immunosuppressive Tumor Microenvironment., PMID:36259590
Engineering an autonomous VH domain to modulate intracellular pathways and to interrogate the eIF4F complex., PMID:35982046
Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells., PMID:35768165
Translational regulation of TFH cell differentiation and autoimmune pathogenesis., PMID:35749506
Digitoxin promotes apoptosis and inhibits proliferation and migration by reducing HIF-1α and STAT3 in KRAS mutant human colon cancer cells., PMID:34717917
Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation., PMID:34445582
Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E., PMID:34013332
Inhibition of mutant KRAS-driven overexpression of ARF6 and MYC by an eIF4A inhibitor drug improves the effects of anti-PD-1 immunotherapy for pancreatic cancer., PMID:34001163
Inhibition Effect of Chloroquine and Integrin-Linked Kinase Knockdown on Translation in Melanoma Cells., PMID:33916175
Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors., PMID:33786785
Modulation of the IGF1R-MTOR pathway attenuates motor neuron toxicity of human ALS SOD1G93A astrocytes., PMID:33749521
PCR Array Technology in Biopsy Samples Identifies Up-Regulated mTOR Pathway Genes as Potential Rejection Biomarkers After Kidney Transplantation., PMID:33681239
Exonuclease resistant 18S and 25S ribosomal RNA components in yeast are possibly newly transcribed by RNA polymerase II., PMID:32738873
Mechanism of AMPK-mediated apoptosis of rat gastric smooth muscle cells under high glucose condition., PMID:31769476
Decreased NOTCH1 Activation Correlates with Response to Ibrutinib in Chronic Lymphocytic Leukemia., PMID:31578228
Ciliary neurotrophic factor signaling in the rat orbitofrontal cortex ameliorates stress-induced deficits in reversal learning., PMID:31553898
Impact of the secretome of activated pancreatic stellate cells on growth and differentiation of pancreatic tumour cells., PMID:30923340
Knockdown of IARS2 Inhibited Proliferation of Acute Myeloid Leukemia Cells by Regulating p53/p21/PCNA/eIF4E Pathway., PMID:30832756
The mTORC1/4E-BP/eIF4E Axis Promotes Antibody Class Switching in B Lymphocytes., PMID:30530594
Epithelial mesenchymal transition induced by the CXCL9/CXCR3 axis through AKT activation promotes invasion and metastasis in tongue squamous cell carcinoma., PMID:29286143
Combined treatment of pancreatic cancer xenograft with 90Y-ITGA6B4-mediated radioimmunotherapy and PI3K/mTOR inhibitor., PMID:29204055
Impact of genetic variations in the MAPK signaling pathway on outcome in metastatic colorectal cancer patients treated with first-line FOLFIRI and bevacizumab: data from FIRE-3 and TRIBE trials., PMID:29045529
Predictability of Recurrence using Immunohistochemistry to delineate Surgical Margins in mucosal Head and Neck Squamous Cell Carcinoma (PRISM-HNSCC): study protocol for a prospective, observational and bilateral study in Australia and India., PMID:29038175
Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-κB signalling of young grass carp (Ctenopharyngodon idella)., PMID:28951222
Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-κB and target of rapamycin signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila., PMID:28820083
Programmed cell death-1 (PD-1) checkpoint blockade in combination with a mammalian target of rapamycin inhibitor restrains hepatocellular carcinoma growth induced by hepatoma cell-intrinsic PD-1., PMID:28732118
The Potyvirus Particle Recruits the Plant Translation Initiation Factor eIF4E by Means of the VPg covalently Linked to the Viral RNA., PMID:28609214
Genome-wide association mapping in winter barley for grain yield and culm cell wall polymer content using the high-throughput CoMPP technique., PMID:28301509
Reciprocal regulation of mTOR complexes in pancreatic islets from humans with type 2 diabetes., PMID:28004151
A Review of Stapled Peptides and Small Molecules to Inhibit Protein-Protein Interactions in Cancer., PMID:27356541
mTORC1 signalling and eIF4E/4E-BP1 translation initiation factor stoichiometry influence recombinant protein productivity from GS-CHOK1 cells., PMID:27760840
Mitotic protein kinase CDK1 phosphorylation of mRNA translation regulator 4E-BP1 Ser83 may contribute to cell transformation., PMID:27402756
Signaling pathways controlling activity-dependent local translation of BDNF and their localization in dendritic arbors., PMID:27270670
Synthetic Polyamines to Regulate mRNA Translation through the Preservative Binding of Eukaryotic Initiation Factor 4E to the Cap Structure., PMID:26811205
Expression of Mammalian Target of Rapamycin and Downstream Targets in Normal and Gestational Diabetic Human Term Placenta., PMID:26335179
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes., PMID:26257270
Identification of the receptor tyrosine kinases (RTKs)-oriented functional targets of miR-206 by an antibody-based protein array., PMID:26143374
Doxorubicin induces apoptosis by targeting Madcam1 and AKT and inhibiting protein translation initiation in hepatocellular carcinoma cells., PMID:26124182
Immunohistochemistry-based prognostic biomarkers in NSCLC: novel findings on the road to clinical use?, PMID:25599124
Measles vaccine strains for virotherapy of non-small-cell lung carcinoma., PMID:25157763
Ampelopsin suppresses breast carcinogenesis by inhibiting the mTOR signalling pathway., PMID:24861637
For research use only. Not for human or drug use.
