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Anti-CD3D Antibody (R2Y69)

Catalog #:   RHC08103 Specific References (13) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, FCM, WB
Accession: P04234
Overview

Catalog No.

RHC08103

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, WB: 1:500-1:2000

Target

T-cell surface glycoprotein CD3 delta chain, CD3D, T-cell receptor T3 delta chain, T3D, CD3d

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P04234

Applications

ELISA, FCM, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2Y69

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using CD3D mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of THP-1 cells using CD3D mouse mAb (green) and negative control (red).
  • Western blot
    Western blot analysis using CD3D mouse mAb against HL-60 (1), and U937 (2) cell lysate.
References

Dual Role of Necroptosis in Cervical Cancer: Promoting Tumor Aggression and Modulating the Immune Microenvironment via the JAK2-STAT3 Pathway., PMID:39247606

JAML immunotherapy targets recently activated tumor-infiltrating CD8+ T cells., PMID:36701231

CD3D, GZMK, and KLRB1 Are Potential Markers for Early Diagnosis of Rheumatoid Arthritis, Especially in Anti-Citrullinated Protein Antibody-Negative Patients., PMID:34603038

Surface Ligand Valency and Immunoliposome Binding: when More Is Not Always Better., PMID:34463936

Identification of CD8+ T Cell-Related Genes: Correlations with Immune Phenotypes and Outcomes of Liver Cancer., PMID:34124275

CTLA-4 blockade and interferon-α induce proinflammatory transcriptional changes in the tumor immune landscape that correlate with pathologic response in melanoma., PMID:33428680

Distinct Gene Expression Patterns between Nasal Mucosal Cells and Blood Collected from Allergic Rhinitis Sufferers., PMID:29920501

Entire CD3ε, δ, and γ humanized mouse to evaluate human CD3-mediated therapeutics., PMID:28368009

Preferential expression of IGHV and IGHD encoding antibodies with exceptionally long CDR3H and a rapid global shift in transcriptome characterizes development of bovine neonatal immunity., PMID:27601209

Differentially expressed gene transcripts using RNA sequencing from the blood of immunosuppressed kidney allograft recipients., PMID:25946140

Human CD3γ, but not CD3δ, haploinsufficiency differentially impairs γδ versus αβ surface TCR expression., PMID:23336327

New alleles of chicken CD8 alpha and CD3d found in Chinese native and western breeds., PMID:17904644

Novel insight into the agonistic mechanism of alefacept in vivo: differentially expressed genes may serve as biomarkers of response in psoriasis patients., PMID:17513795

Datasheet

Document Download

Anti-CD3D Antibody (R2Y69).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-CD3D Antibody (R2Y69) [RHC08103]
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