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Anti-Rat IgG2a Antibody (R2X68), HRP

Catalog #:   RRB96702 Specific References (49) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: WB
Accession: P20760
Overview

Catalog No.

RRB96702

Species reactivity

Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Conjugation

HRP

Tested applications

WB: 1:500-1:1000

Target

IgG2a

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P20760

Applications

WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.01% Thimerosal.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2X68

Data Image
  • Western blot
    Lane 1: 100 ng of rat IgG1 Kappa; Lane 2: 100 ng of rat IgG2a Kappa; Lane 3: 100 ng of rat IgG2b Kappa; Lane 4: 100 ng of rat IgG2c Kappa; Lane 5: 100 ng of rat IgA; Lane 6: 100 ng of rat IgM; Lane 7: 100 ng of rat IgG2a Lambda; Lane 8: 100 ng of mouse IgG1; Lane 9: 100 ng of mouse IgG2a; Lane 10: 100 ng of mouse IgG2b; Lane 11: 100 ng of mouse IgG3; Lane 12: 100 ng of mouse IgA; Lane 13: 100 ng of mouse IgM ;Result: RRB96702 can detect Rat IgG2a by Western blotting.
References

Quantitation of total antibody (tAb) from antibody drug conjugate (ADC) PYX-201 in rat and monkey plasma using an enzyme-linked immunosorbent assay (ELISA) and its application in preclinical studies., PMID:37167766

Cross-reaction between mouse and rat immunoglobulin G: does it matter in sandwich ELISA?, PMID:34403025

Experimental and bioinformatic approach to identifying antigenic epitopes in human α- and β-enolases., PMID:29922723

Specific chemiluminescent protocol for dual-site recognition of Streptococcus mutans utilizing strong affinity between teicoplanin and Gram-positive bacteria., PMID:29310243

A capillary based chemiluminscent multi-target immunoassay., PMID:25731812

Neuron-derived IgG protects dopaminergic neurons from insult by 6-OHDA and activates microglia through the FcγR I and TLR4 pathways., PMID:23791745

An ultrasensitive electrochemical immunosensor platform with double signal amplification for indole-3-acetic acid determinations in plant seeds., PMID:23377501

Origin of the apical transcytic membrane system in jejunal absorptive cells of neonates., PMID:21717309

Substrate specificity of rat sera IgG antibodies with peroxidase and oxidoreductase activities., PMID:16835846

An impedance array biosensor for detection of multiple antibody-antigen interactions., PMID:16732363

Metal ions-dependent peroxidase and oxidoreductase activities of polyclonal IgGs from the sera of Wistar rats., PMID:16416456

The presence of 4-hydroxynonenal/protein complex as an indicator of oxidative stress after experimental spinal cord contusion in a rat model., PMID:9576257

Immunoglobulin G is associated with surfactant protein A aggregate isolated from patients with pulmonary alveolar proteinosis., PMID:9154892

Intestinal transport and processing of immunoglobulin G in the neonatal and adult rat., PMID:7647150

Immunocytochemical localization of immunoglobulins in the rat brain: relationship to the blood-brain barrier., PMID:8040362

Blood-brain barrier changes following intracerebral injection of human recombinant tumor necrosis factor-alpha in the rat., PMID:7807180

Serum proteins bypass the blood-brain fluid barriers for extracellular entry to the central nervous system., PMID:8491281

A one-step immunohistochemical method for detection of blood-brain barrier disturbances for immunoglobulins in lesioned rat brain with special reference to false-positive labelling in immunohistochemistry., PMID:8474256

Feeding neonatal rats with IgG antibodies leads to humoral hyporesponsiveness in the adult., PMID:1385314

Immunoelectron microscopic localization of laminin in rat ovarian follicles., PMID:1605377

Immunocytochemical staining of neuropeptides in terminal arborization of primary afferent fibers anterogradely labeled and identified at light and electron microscopic levels., PMID:1383643

Breakdown of the blood-brain barrier after fluid percussive brain injury in the rat. Part 1: Distribution and time course of protein extravasation., PMID:1619673

Breakdown of the blood-brain barrier after fluid percussion brain injury in the rat: Part 2: Effect of hypoxia on permeability to plasma proteins., PMID:1291693

Two distinct monoclonal antibodies raised against mouse beta nerve growth factor. Generation of bi-specific anti-nerve growth factor anti-horseradish peroxidase antibodies for use in a homogeneous enzyme immunoassay., PMID:1999653

Development of a bi-specific monoclonal antibody for simultaneous detection of rabbit IgG and horseradish peroxidase: use as a general reagent in immunocytochemistry and enzyme-linked immunosorbent assay., PMID:2299180

Permeability of the normal rat brain, spinal cord and dorsal root ganglia microcirculations to immunoglobulins G., PMID:2317595

Production of a bi-specific monoclonal antibody recognizing mouse kappa light chains and horseradish peroxidase. Applications in immunoassays., PMID:2127927

Morphological localization of a major lysosomal membrane glycoprotein in the endocytic membrane system., PMID:2691512

Biochemical analysis of the movement of a major lysosomal membrane glycoprotein in the endocytic membrane system., PMID:2606917

Measurement of the rates of basal pinocytosis of horseradish peroxidase and internalization of heat-aggregated IgG by macrophages from normal and streptozotocin-induced diabetic rats., PMID:3061933

Foreign and endogenous serum protein extravasation during harmaline tremors or kainic acid seizures in the rat: a comparison., PMID:3140571

The pathophysiology of protein-overload proteinuria., PMID:3661680

The permeability of the primary decidual zone in the rat uterus: an ultrastructural tracer and freeze-fracture study., PMID:3814692

Double-enzyme conjugates, producing an intermediate color, for simultaneous and direct detection of three different intracellular immunoglobulin determinants with only two enzymes., PMID:2419394

Inhalation of horseradish peroxidase by rabbits with specific IgE antibodies results in release into the circulation of platelet-activating factor and in lung lesions., PMID:3971603

Distribution of laminin within rat and mouse renal, splenic, intestinal, and hepatic basement membranes identified after the intravenous injection of heterologous antilaminin IgG., PMID:3881623

Electron-microscopic immunohistochemical study of the localization of immunoglobulin G in the choroid plexus of the rat., PMID:6386170

Laminin in glomerular basement membranes of aminonucleoside nephrotic rats. Increased proteinuria induced by antilaminin immunoglobulin G., PMID:6865329

A new experimental method for the dynamic study of the antibody transfer mechanism from mother to fetus in the rat., PMID:6878185

Proteinuria and structural alterations in rat glomerular basement membranes induced by intravenously injected anti-laminin immunoglobulin G., PMID:7086353

Permeability of the blood-air barrier to antiperoxidase antibodies and their fragments in the normal rat lung., PMID:7091880

The mechanisms of blood-brain barrier impairment by hyperosmolar perfusion. An electron cytochemical study comparing exogenous HRP and endogenous antibody to HRP as tracers., PMID:7064667

Evidence for the sorting of endocytic vesicle contents during the receptor-mediated transport of IgG across the newborn rat intestine., PMID:7298722

The permeability of the blood-brain barrier in acute hypertension. Comparison of an endogenous and exogenous protein tracer., PMID:6939220

Pathways followed by membrane recovered from the surface of plasma cells and myeloma cells., PMID:6156978

Distribution of immunoglobulin G receptors in the small intestine of the young rat., PMID:7364873

Accelerated immune complex nephritis due to mesangial overloading in spontaneous hypertensive (SHR) rats., PMID:161594

Intestinal absorption of immune complexes by neonatal rats: a route of antigen transfer from mother to young., PMID:493961

Fate of plasma membrane during endocytosis. II. Evidence for recycling (shuttle) of plasma membrane constituents., PMID:479310

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-Rat IgG2a Antibody (R2X68), HRP [RRB96702]
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