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Anti-NDUFB8 Antibody (R2W18)

Catalog #:   RHB55302 Specific References (2) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC, IP, WB
Accession: O95169
Overview

Catalog No.

RHB55302

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:25-1:200, IP: 1:100-1:200, WB: 1:500-1:1000

Target

NADH-ubiquinone oxidoreductase ASHI subunit, NDUFB8, CI-ASHI, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial, Complex I-ASHI

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O95169

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2W18

Data Image
  • Immunohistochemical
    IHC-P analysis of heart muscle tissue by NDUFB8 antibody (RHB55302). IHC-P was performed using sections of the formalin-fixed paraffin-embedded heart muscle tissue;Result: Myocytes are positively stained at the cytoplasm.
  • Immunoprecipitation
    Lane 1: Hep G2 lysate; Lane 2: NDUFB8 immunoprecipitated from Hep G2 lysate by RHB55302;Lane 3: The same as Lane 2 but use IgG isotype control antibody ;Result: RHB55302 can immunoprecipitate NDUFB8;
  • Western blot
    Lane 1: 15 μg of HeLa lysate; Lane 2: 15 μg of Hep G2 lysate; Lane 3: 15 μg of THP-1 lysate; Lane 4: 15 μg of U-251MG lysate; Lane 5: 15 μg of A549 lysate; Lane 6: 15 μg of rat brain tissue lysate; Lane 7: 15 μg of mouse brain tissue lysate; Lane 8: 15 μg of mouse heart tissue lysate; Lane 9: 15 μg of HEK-293 lysate; Lane 10: 15 μg of Caco-2 lysate; Lane 11: 15 μg of HL-60 lysate ;Result: RHB55302 can detect NDUFB8 by Western blotting.
References

Loss of cholinergic receptor muscarinic 1 impairs cortical mitochondrial structure and function: implications in Alzheimer's disease., PMID:37274741

Using a quantitative quadruple immunofluorescent assay to diagnose isolated mitochondrial Complex I deficiency., PMID:29142257

Datasheet

Document Download

Anti-NDUFB8 Antibody (R2W18).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-NDUFB8 Antibody (R2W18) [RHB55302]
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