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Anti-PLIN3 Antibody (R2V33)

Catalog #:   RHA96801 Specific References (16) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: FCM, IF, WB
Accession: O60664
Overview

Catalog No.

RHA96801

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:100-1:500, WB: 1:500-1:2000

Target

47 kDa mannose 6-phosphate receptor-binding protein, M6PRBP1, 47 kDa MPR-binding protein, PLIN3, Mannose-6-phosphate receptor-binding protein 1, Cargo selection protein TIP47, Perilipin-3, PP17, TIP47, Placental protein 17

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O60664

Applications

FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2V33

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using PLIN3 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using PLIN3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using PLIN3 mouse mAb against THP-1 (1), HepG2 (2), K562 (3) and Hela (4) cell lysate.
References

Choline kinase alpha 2 acts as a protein kinase to promote lipolysis of lipid droplets., PMID:34077757

Oleic acid in the absence of a PPARγ agonist increases adipogenic gene expression in bovine muscle satellite cells1., PMID:31424542

Delayed recruiting of TPD52 to lipid droplets - evidence for a "second wave" of lipid droplet-associated proteins that respond to altered lipid storage induced by Brefeldin A treatment., PMID:31278300

On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex., PMID:24587346

A Toll-like receptor 9-mediated pathway stimulates perilipin 3 (TIP47) expression and induces lipid accumulation in macrophages., PMID:20628022

Preliminary study of TIP47 as a possible new biomarker of cervical dysplasia and invasive carcinoma., PMID:19331227

Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100., PMID:18577578

All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 to lipid droplets in the retinal pigment epithelium., PMID:17525222

Immunohistochemical staining for adipophilin, perilipin and TIP47., PMID:16556662

Spatial integration of TIP47 and adipophilin in macrophage lipid bodies., PMID:15545278

A high-throughput proteo-genomics method to identify antibody targets associated with malignant disease., PMID:15137953

Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells., PMID:12631276

Functional conservation for lipid storage droplet association among Perilipin, ADRP, and TIP47 (PAT)-related proteins in mammals, Drosophila, and Dictyostelium., PMID:12077142

Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells., PMID:11827983

TIP47 is not a component of lipid droplets., PMID:11313361

TIP47 associates with lipid droplets., PMID:11084026

Datasheet

Document Download

Anti-PLIN3 Antibody (R2V33).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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For research use only. Not for human or drug use.

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Anti-PLIN3 Antibody (R2V33) [RHA96801]
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