Catalog No.
RHA88904
Species reactivity
Human, Monkey
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
Lysine-specific histone demethylase 1A, KDM1, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, BRAF35-HDAC complex protein BHC110, KDM1A, AOF2, LSD1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O60341
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R2V20
Lysine-specific demethylase 1 deletion reshapes tumour microenvironment to overcome acquired resistance to anti-programmed death 1 therapy in liver cancer., PMID:40356247
LSD1+8a is an RNA biomarker of neuroendocrine prostate cancer., PMID:40088674
Targeting intracellular proteins with cell type-specific functions for cancer immunotherapy., PMID:39872303
Inhibiting Lysine Demethylase 1A Improves L1CAM-Specific CAR T Cell Therapy by Unleashing Antigen-Independent Killing via the FAS-FASL Axis., PMID:34771652
Targeting LSD1 suppresses stem cell-like properties and sensitizes head and neck squamous cell carcinoma to PD-1 blockade., PMID:34689153
Phase Ib study of patients with metastatic castrate-resistant prostate cancer treated with different sequencing regimens of atezolizumab and sipuleucel-T., PMID:34376554
LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer., PMID:33731702
Targeting Nuclear LSD1 to Reprogram Cancer Cells and Reinvigorate Exhausted T Cells via a Novel LSD1-EOMES Switch., PMID:32612611
Inhibition of histone lysine-specific demethylase 1 elicits breast tumor immunity and enhances antitumor efficacy of immune checkpoint blockade., PMID:30111819
Identification of Rpl29 as a major substrate of the lysine methyltransferase Set7/9., PMID:29959229
LSD1 mediates metabolic reprogramming by glucocorticoids during myogenic differentiation., PMID:29618057
Germline Lysine-Specific Demethylase 1 (LSD1/KDM1A) Mutations Confer Susceptibility to Multiple Myeloma., PMID:29559475
RORα2 requires LSD1 to enhance tumor progression in breast cancer., PMID:28931919
Epigenetic regulation of puberty via Zinc finger protein-mediated transcriptional repression., PMID:26671628
Primary Cilia on Horizontal Basal Cells Regulate Regeneration of the Olfactory Epithelium., PMID:26446227
Measurement of lysine-specific demethylase-1 activity in the nuclear extracts by flow-injection based time-of-flight mass spectrometry., PMID:25759518
Regulation of tissue factor pathway inhibitor-2 (TFPI-2) expression by lysine-specific demethylase 1 and 2 (LSD1 and LSD2)., PMID:25036127
Heterogeneous Antibody-Based Activity Assay for Lysine Specific Demethylase 1 (LSD1) on a Histone Peptide Substrate., PMID:24687155
Dynamic patterns of histone H3 lysine 4 methyltransferases and demethylases during mouse preimplantation development., PMID:24619213
Inhibiting interactions of lysine demethylase LSD1 with snail/slug blocks cancer cell invasion., PMID:23054398
Novel colorimetric assay of LSD1 activity using gold nanoparticles., PMID:22534778
A sensitive, homogeneous, and high-throughput assay for lysine-specific histone demethylases at the H3K4 site., PMID:22192306
Screening assays for epigenetic targets using native histones as substrates., PMID:21965113
Development of homogeneous nonradioactive methyltransferase and demethylase assays targeting histone H3 lysine 4., PMID:21940714
Activation of HIV transcription by the viral Tat protein requires a demethylation step mediated by lysine-specific demethylase 1 (LSD1/KDM1)., PMID:21876670
High-throughput TR-FRET assays for identifying inhibitors of LSD1 and JMJD2C histone lysine demethylases., PMID:21859682
Screening for lysine-specific demethylase-1 inhibitors using a label-free high-throughput mass spectrometry assay., PMID:21855527
Engagement of the lysine-specific demethylase/HDAC1/CoREST/REST complex by herpes simplex virus 1., PMID:19193804
Involvement of histone demethylase LSD1 in Blimp-1-mediated gene repression during plasma cell differentiation., PMID:19124609
For research use only. Not for human or drug use.
