Catalog No.
RHN74701
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
DBF4-type zinc finger-containing protein 1, Chiffon homolog A, Protein DBF4 homolog A, ZDBF1, Activator of S phase kinase, DBF4A, DBF4, ASK
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q9UBU7
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2R17
Inhibition of Cdc7/Dbf4 kinase activity affects specific phosphorylation sites on MCM2 in cancer cells., PMID:18286467
Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells., PMID:16899510
Sequence requirements for function of the Drosophila chorion gene locus ACE3 replicator and ori-beta origin elements., PMID:15105371
A generic time-resolved fluorescence assay for serine/threonine kinase activity: application to Cdc7/Dbf4., PMID:12895303
A 63-base pair DNA segment containing an Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinase., PMID:12015319
Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication., PMID:10523313
Cell cycle regulation of DNA replication initiator factor Dbf4p., PMID:10330168
Oligomers of the Cdc7/Dbf4 protein kinase exist in the yeast cell., PMID:9790600
Preparation of active Cdc7/Dbf4 kinase from yeast cells., PMID:9251036