Catalog No.
RHJ72103
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:5000
Target
Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, MBD2, DMTase, Demethylase
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q9UBB5
Applications
FCM, IF, IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2K58
MBD2 promotes B cell differentiation and BCR signaling in systemic lupus erythematosus by regulating the LEF-1-PTEN-PI3K axis., PMID:40467564
Aged mice-derived bronchial epithelial cells regulate Th17 cell differentiation in asthma via the MBD2-sICOSL axis., PMID:40252480
YAP/TAZ are crucial regulators of macrophage-mediated pulmonary inflammation and fibrosis after bleomycin-induced injury., PMID:39915054
Epigenetic modifier alpha-ketoglutarate modulates aberrant gene body methylation and hydroxymethylation marks in diabetic heart., PMID:37101286
Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin., PMID:36932919
A CRISPR/Cas9-mediated screen identifies determinants of early plasma cell differentiation., PMID:36685499
Dendritic cells' characteristics in patients with treated systemic lupus erythematosus., PMID:32730703
Melatonin-induced suppression of DNA methylation promotes odontogenic differentiation in human dental pulp cells., PMID:32718272
Alterations in peripheral blood B cells in systemic lupus erythematosus patients with renal insufficiency., PMID:32248020
Promoter CpG-Site Methylation of the KAI1 Metastasis Suppressor Gene Contributes to Its Epigenetic Repression in Prostate Cancer., PMID:27813113
Anti-tumor angiogenesis effect of genetic fusion vaccine encoding murine beta-defensin 2 and tumor endothelial marker-8 in a CT-26 murine colorectal carcinoma model., PMID:26064415
Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine., PMID:25888776
Murine β-defensin-2 may regulate the effect of bacillus Calmette-Guérin (BCG) in normal mouse bladder., PMID:25573055
Mechanical loading in osteocytes induces formation of a Src/Pyk2/MBD2 complex that suppresses anabolic gene expression., PMID:24841674
Beta-defensin 2 enhances immunogenicity and protection of an adenovirus-based H5N1 influenza vaccine at an early time., PMID:24051000
Transcription onset of genes critical in liver carcinogenesis is epigenetically regulated by methylated DNA-binding protein MBD2., PMID:23955541
Effects of ultraviolet B exposure on DNA methylation in patients with systemic lupus erythematosus., PMID:23596493
Epigenetic dysregulation in salivary glands from patients with primary Sjögren's syndrome may be ascribed to infiltrating B cells., PMID:23478041
Enrichment of methylated DNA by methyl-CpG immunoprecipitation., PMID:23296965
The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos., PMID:23154201
MBD2 and multiple domains of CHD4 are required for transcriptional repression by Mi-2/NuRD complexes., PMID:23071088
Epigenetic regulation of CD133/PROM1 expression in glioma stem cells by Sp1/myc and promoter methylation., PMID:22945648
Effects of long-term low-dose formaldehyde exposure on global genomic hypomethylation in 16HBE cells., PMID:21745553
TREM-1 amplifies corneal inflammation after Pseudomonas aeruginosa infection by modulating Toll-like receptor signaling and Th1/Th2-type immune responses., PMID:21555403
Global DNA methylation, DNMT1, and MBD2 in patients with rheumatoid arthritis., PMID:20937307
Label-free DNA methylation analysis using opto-fluidic ring resonators., PMID:20846848
Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications., PMID:20565761
Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine., PMID:20371518
[Immune effects of four coxsackievirus B3 VP1 DNA fusion vaccines in mice]., PMID:20230664
Promoter demethylation and chromatin remodeling by green tea polyphenols leads to re-expression of GSTP1 in human prostate cancer cells., PMID:19856314
In vitro detection of methylated DNA via recombinant protein MBD2b., PMID:18987990
Innate immune responses in murine pleural mesothelial cells: Toll-like receptor-2 dependent induction of beta-defensin-2 by staphylococcal peptidoglycan., PMID:18621910
Immunity against tumor angiogenesis induced by a fusion vaccine with murine beta-defensin 2 and mFlk-1., PMID:18006780
Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses., PMID:16424055
Expression, purification and characterization of methyl DNA binding protein from Bombyx mori., PMID:16299598
The binding sites for the chromatin insulator protein CTCF map to DNA methylation-free domains genome-wide., PMID:15256511
Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression., PMID:14610093
Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns., PMID:14519686
Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3., PMID:12183469
Cancer-related serological recognition of human colon cancer: identification of potential diagnostic and immunotherapeutic targets., PMID:12124339
DNA methyltransferase and demethylase in human prostate cancer., PMID:11870882
Dynamic reprogramming of DNA methylation in the early mouse embryo., PMID:11784103
MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase., PMID:10947852
Active repression of methylated genes by the chromosomal protein MBD1., PMID:10648624
MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex., PMID:10471499
For research use only. Not for human or drug use.
