Catalog No.
RHF93001
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
Vitamin D(3) 25-hydroxylase, Cytochrome P-450C27/25, CYP27A1, Cytochrome P450 27, CYP27, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, Sterol 27-hydroxylase, Sterol 26-hydroxylase, mitochondrial
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q02318
Applications
FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2C18
Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes., PMID:36498935
Down-regulation of CYP27B1 gene expression in Iranian patients with relapsing-remitting multiple sclerosis., PMID:27792005
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer., PMID:27341022
A rare variant in CYP27A1 and its association with atopic dermatitis with high serum total IgE., PMID:27259383
Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy., PMID:22903823
Expression of Th2-skewed pathology mediators in monocyte-derived type 2 of dendritic cells (DC2)., PMID:19643136
Interferon-gamma impedes reverse cholesterol transport and promotes foam cell transformation in THP-1 human monocytes/macrophages., PMID:15507847
CYP3A4 is a human microsomal vitamin D 25-hydroxylase., PMID:15005856
Metabolism of 25-hydroxyvitamin D3 by microsomal and mitochondrial vitamin D3 25-hydroxylases (CYP2D25 and CYP27A1): a novel reaction by CYP27A1., PMID:12782149
Immune complexes and IFN-gamma decrease cholesterol 27-hydroxylase in human arterial endothelium and macrophages., PMID:11714861
Vitamin D and prostate cancer., PMID:11384870
Cytochrome P450 enzymes in the bioactivation of vitamin D to its hormonal form (review)., PMID:11172626
A potential role for sterol 27-hydroxylase in atherogenesis., PMID:11166758
Identification of 25-hydroxyvitamin D3 1alpha-hydroxylase gene expression in macrophages., PMID:10916079
A highly sensitive method for large-scale measurements of 1,25-dihydroxyvitamin D., PMID:9448854
A possible role for CYP27 as a major renal mitochondrial 25-hydroxyvitamin D3 1 alpha-hydroxylase., PMID:8706816
Liver mitochondrial cytochrome P450 CYP27 and recombinant-expressed human CYP27 catalyze 1 alpha-hydroxylation of 25-hydroxyvitamin D3., PMID:7937829
1 alpha, 25-Dihydroxyvitamin D3 and 19-nor-1 alpha, 25-dihydroxyvitamin D2 suppress immunoglobulin production and thymic lymphocyte proliferation in vivo., PMID:8251528
Purification and characterization of a vitamin D3 25-hydroxylase from pig liver microsomes., PMID:1445236
Molecular cloning of cDNA for vitamin D3 25-hydroxylase from rat liver mitochondria., PMID:2318307
Monoclonal antibodies to chick renal calcium regulating hemeproteins. Biochemical and immunohistochemical interactions with mitochondrial 25-hydroxyvitamin D3 hydroxylases., PMID:1726440
Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes., PMID:2460437
Quantitative analysis of the mitochondrial cytochrome P-450-linked monooxygenase system: NADPH-hepatoredoxin reductase, hepatoredoxin, and cytochrome P-450s27 in livers of patients with cerebrotendinous xanthomatosis., PMID:3791635
For research use only. Not for human or drug use.
