Catalog No.
RHE73701
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4, DNA ligase 4
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P49917
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1X90
DNA Ligase 4 Inhibition Sensitizes Prostate Cancer to Immune Checkpoint Blockade In Vivo., PMID:40037856
The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signaling., PMID:36473865
DNA ligase IV dificiency with elevated serum IgG levels suspected to have myelodysplastic syndrome: a case report., PMID:36221079
DNA damage and repair in the visual center in the rhesus monkey model of glaucoma., PMID:35364099
X-ray scattering reveals disordered linkers and dynamic interfaces in complexes and mechanisms for DNA double-strand break repair impacting cell and cancer biology., PMID:34056803
Structural basis of long-range to short-range synaptic transition in NHEJ., PMID:33854234
Elucidating the impact of cottonseed hydrolysates on CHO cell culture performance through transcriptomic analysis., PMID:33201275
Temporally uncoupled signal and coding joint formation in human V(D)J recombination., PMID:33157352
Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire., PMID:31875554
Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies., PMID:30395541
Unique heterozygous presentation in an infant with DNA ligase IV syndrome., PMID:28866308
Effects of DNA end configuration on XRCC4-DNA ligase IV and its stimulation of Artemis activity., PMID:28696258
Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α., PMID:27068747
In cellulo phosphorylation of XRCC4 Ser320 by DNA-PK induced by DNA damage., PMID:26666690
The DNA Ligase IV Syndrome R278H Mutation Impairs B Lymphopoiesis via Error-Prone Nonhomologous End-Joining., PMID:26608917
DNA Ligases I and III Support Nucleotide Excision Repair in DT40 Cells with Similar Efficiency., PMID:26131740
Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair., PMID:25941401
A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells., PMID:25879709
Localization of SUCLA2 and SUCLG2 subunits of succinyl CoA ligase within the cerebral cortex suggests the absence of matrix substrate-level phosphorylation in glial cells of the human brain., PMID:25370487
Enhanced non-homologous end joining contributes toward synthetic lethality of pathological RAD51C mutants with poly (ADP-ribose) polymerase., PMID:25292178
Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay., PMID:25063296
Hypoxia enhances the radioresistance of mouse mesenchymal stromal cells., PMID:24578291
Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines., PMID:24530422
[Neuroimmunological diseases associated with VGKC complex antibodies]., PMID:23777104
ID1 affects the efficacy of radiotherapy in glioblastoma through inhibition of DNA repair pathways., PMID:23377983
Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair., PMID:22941596
Targeting abnormal DNA double-strand break repair in tyrosine kinase inhibitor-resistant chronic myeloid leukemias., PMID:22641215
Partial complementation of a DNA ligase I deficiency by DNA ligase III and its impact on cell survival and telomere stability in mammalian cells., PMID:22460582
Cernunnos influences human immunoglobulin class switch recombination and may be associated with B cell lymphomagenesis., PMID:22312109
Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1., PMID:21781197
Enzymatic assembly of overlapping DNA fragments., PMID:21601685
Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair., PMID:21256093
Alternative end-joining catalyzes class switch recombination in the absence of both Ku70 and DNA ligase 4., PMID:20142431
Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70., PMID:20133803
Homozygous DNA ligase IV R278H mutation in mice leads to leaky SCID and represents a model for human LIG4 syndrome., PMID:20133615
DNA-PKcs regulates a single-stranded DNA endonuclease activity of Artemis., PMID:20117966
Association between total immunoglobulin E and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes., PMID:19604268
DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories., PMID:19597347
Impaired lymphocyte development and antibody class switching and increased malignancy in a murine model of DNA ligase IV syndrome., PMID:19451691
The human set and transposase domain protein Metnase interacts with DNA Ligase IV and enhances the efficiency and accuracy of non-homologous end-joining., PMID:18773976
ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1., PMID:18754770
Human DNA ligases I and III, but not ligase IV, are required for microhomology-mediated end joining of DNA double-strand breaks., PMID:18440984
Mechanism and regulation of class switch recombination., PMID:18370922
IgH class switching and translocations use a robust non-classical end-joining pathway., PMID:17713479
Evaluation of NAD(+) -dependent DNA ligase of mycobacteria as a potential target for antibiotics., PMID:17548501
A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses., PMID:17396150
Hepatic HNF4alpha deficiency induces periportal expression of glutamine synthetase and other pericentral enzymes., PMID:17256722
Expression of a mutant p193/CUL7 molecule confers resistance to MG132- and etoposide-induced apoptosis independent of p53 or Parc binding., PMID:17229476
Base excision repair proteins are required for integrin-mediated suppression of bleomycin-induced DNA breakage in murine lung endothelial cells., PMID:17202402
PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery., PMID:16924674
For research use only. Not for human or drug use.
