Catalog No.
RHE73702
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4, DNA ligase 4
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P49917
Applications
IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1X89
DNA Ligase 4 Inhibition Sensitizes Prostate Cancer to Immune Checkpoint Blockade In Vivo., PMID:40037856
X-ray scattering reveals disordered linkers and dynamic interfaces in complexes and mechanisms for DNA double-strand break repair impacting cell and cancer biology., PMID:34056803
Temporally uncoupled signal and coding joint formation in human V(D)J recombination., PMID:33157352
Generation of a Mouse Model Lacking the Non-Homologous End-Joining Factor Mri/Cyren., PMID:31795137
Effects of DNA end configuration on XRCC4-DNA ligase IV and its stimulation of Artemis activity., PMID:28696258
The DNA Ligase IV Syndrome R278H Mutation Impairs B Lymphopoiesis via Error-Prone Nonhomologous End-Joining., PMID:26608917
Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair., PMID:25941401
Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines., PMID:24530422
Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair., PMID:21256093
Impaired induction of DNA lesions during immunoglobulin class-switch recombination in humans influences end-joining repair., PMID:21135220
Alternative end-joining catalyzes class switch recombination in the absence of both Ku70 and DNA ligase 4., PMID:20142431
Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70., PMID:20133803
Homozygous DNA ligase IV R278H mutation in mice leads to leaky SCID and represents a model for human LIG4 syndrome., PMID:20133615
Association between total immunoglobulin E and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes., PMID:19604268
Omenn syndrome: inflammation in leaky severe combined immunodeficiency., PMID:18992930
The human set and transposase domain protein Metnase interacts with DNA Ligase IV and enhances the efficiency and accuracy of non-homologous end-joining., PMID:18773976
Human DNA ligases I and III, but not ligase IV, are required for microhomology-mediated end joining of DNA double-strand breaks., PMID:18440984
A severe form of human combined immunodeficiency due to mutations in DNA ligase IV., PMID:16585603
Severe combined immunodeficiency and microcephaly in siblings with hypomorphic mutations in DNA ligase IV., PMID:16358361
Both V(D)J coding ends but neither signal end can recombine at the bcl-2 major breakpoint region, and the rejoining is ligase IV dependent., PMID:16024785
Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells., PMID:15657289
A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts., PMID:15199173
Biochemical evidence for Ku-independent backup pathways of NHEJ., PMID:12954774
In vitro and in vivo interactions of DNA ligase IV with a subunit of the condensin complex., PMID:12589063
Identification of human autoantibodies to the DNA ligase IV/XRCC4 complex and mapping of an autoimmune epitope to a potential regulatory region., PMID:12218164
Autoantibodies against DNA double-strand break repair proteins., PMID:11689355
Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells., PMID:11292837
Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV., PMID:9823897
DNA ligase IV from HeLa cell nuclei., PMID:8798671
For research use only. Not for human or drug use.
