Catalog No.
RHD23803
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
UBF, UBF-1, Nucleolar transcription factor 1, UBF1, Autoantigen NOR-90, UBTF, Upstream-binding factor 1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P17480
Applications
IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1Q41
Electron tomography reveals changes in spatial distribution of UBTF1 and UBTF2 isoforms within nucleolar components during rRNA synthesis inhibition., PMID:31479756
UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation., PMID:30610403
Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription., PMID:29712969
Phosphorylated nucleolar Tau protein is related to the neuronal in vitro differentiation., PMID:29684490
PICT-1 triggers a pro-death autophagy through inhibiting rRNA transcription and AKT/mTOR/p70S6K signaling pathway., PMID:27729611
Altered machinery of protein synthesis is region- and stage-dependent and is associated with α-synuclein oligomers in Parkinson's disease., PMID:26621506
Interpretation of an Extended Autoantibody Profile in a Well-Characterized Australian Systemic Sclerosis (Scleroderma) Cohort Using Principal Components Analysis., PMID:26246178
C6orf89 encodes three distinct HDAC enhancers that function in the nucleolus, the golgi and the midbody., PMID:23460338
[Systemic sclerosis autoantibodies: what dermatologists must know]., PMID:23395500
A quantitative analysis of transcriptionally active syncytiotrophoblast nuclei across human gestation., PMID:21883201
UBF levels determine the number of active ribosomal RNA genes in mammals., PMID:19103806
Pontin is localized in nucleolar fibrillar centers., PMID:18548265
RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor., PMID:16809778
Nucleolar protein upstream binding factor is sequestered into adenovirus DNA replication centres during infection without affecting RNA polymerase I location or ablating rRNA synthesis., PMID:16763197
Acetylation of UBF changes during the cell cycle and regulates the interaction of UBF with RNA polymerase I., PMID:16582105
[Ultrastructural localization of transcription sites of rRNA genes in the nucleolus of wheat]., PMID:15344322
Immunolocalization of upstream binding factor and pocket protein p130 during final stages of bovine oocyte growth., PMID:14613906
Autoantibodies to NOR 90/hUBF: longterm clinical and serological followup in a patient with limited systemic sclerosis suggests an antigen driven immune response., PMID:12136917
Autoantibody responses in Chinese hepatocellular carcinoma., PMID:11998899
Distribution and transcription activity of nucleolar DNA in higher plant cells., PMID:11913961
The role of acetylation in rDNA transcription., PMID:11600700
Immunolocalization of nucleolar proteins during bovine oocyte growth, meiotic maturation, and fertilization., PMID:11319160
Functional and molecular reorganization of the nucleolar apparatus in maturing mouse oocytes., PMID:10882521
Behavior of nucleolar proteins during the course of apoptosis in camptothecin-treated HL60 cells., PMID:10842321
Ultrastructural analysis of nucleolar transcription in cells microinjected with 5-bromo-UTP., PMID:10817672
The mitotically phosphorylated form of the transcription termination factor TTF-1 is associated with the repressed rDNA transcription machinery., PMID:10504331
Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A)., PMID:10413677
The DNA binding domains of UBF represent major human autoepitopes conserved in vertebrates species., PMID:10230738
Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein., PMID:9843972
The relationship of ASE-1 and NOR-90 in autoimmune sera., PMID:9818653
Nuclear distribution of human DNA topoisomerase IIbeta: a nuclear targeting signal resides in the 116-residue C-terminal tail., PMID:9743583
Nucleolar function and size in cancer cells., PMID:9588897
Immunohistochemical detection of ribosomal transcription factor UBF: diagnostic value in malignant specimens., PMID:9582531
Mammalian RNA polymerase I exists as a holoenzyme with associated basal transcription factors., PMID:9451438
Association of the nucleolar transcription factor UBF with the transcriptionally inactive rRNA genes of pronuclei and early Xenopus embryos., PMID:9378756
Specific interaction between human kinetochore protein CENP-C and a nucleolar transcriptional regulator., PMID:8702533
Detection of autoantibodies to nucleolar transcription factor NOR 90/hUBF in sera of patients with rheumatic diseases, by recombinant autoantigen-based assays., PMID:8702439
Intracellular distribution of HMG1, HMG2 and UBF change following treatment with cisplatin., PMID:8679707
RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription., PMID:8641287
The rDNA transcription machinery is assembled during mitosis in active NORs and absent in inactive NORs., PMID:8609158
In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive., PMID:8609157
The species-specific RNA polymerase I transcription factor SL-1 binds to upstream binding factor., PMID:8552083
The RNA polymerase I transcription factor UBF and rDNA are located at the same major sites in both interphase and mitotic pig embryonic kidney (PK) cells., PMID:8751374
Autoantibodies to the nucleolar organizer antigen NOR-90 in children with systemic rheumatic diseases., PMID:7783073
Clinical relevance and HLA association of autoantibodies against the nucleolus organizer region (NOR-90)., PMID:7699685
Molecular structure and function of autoantigens in systemic sclerosis., PMID:7650417
[The immunolocalization of the ribosomal gene transcription initiation factor UBF in the interphase and mitosis]., PMID:7571017
Immunodetection of the ribosomal transcription factor UBF at the nucleolus organizer regions of fish cells., PMID:7954874
Immunocytochemical characterization of human NOR-90 (upstream binding factor) and associated antigens reactive with autoimmune sera. Two MR forms of NOR-90/hUBF autoantigens., PMID:8072492
The RNA polymerase I-specific transcription initiation factor UBF is associated with transcriptionally active and inactive ribosomal genes., PMID:8306821
For research use only. Not for human or drug use.
