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Anti-Phospho-Histone H2A.X (Ser139) Antibody (R1P91)

Catalog #:   RHD15008 Specific References (18) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IP, WB
Accession: P16104
Overview

Catalog No.

RHD15008

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IP: 1:20, WB: 1:500-1:1000

Target

H2a/x, Histone H2A.X, H2AFX, Histone H2AX, H2AX

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P16104

Applications

IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1P91

Data Image
  • Western blot
    Western blot analysis of gamma H2A.X (Phospho-Ser139) in HepG2 lysates using Phospho-Histone H2A.X (Ser139) antibody.
  • Western blot
    Western blot analysis of gamma H2A.X (Phospho-Ser139) in Jurkat, THP-1, C6, 3T3 lysates using gamma H2A.X (Phospho-Ser139) antibody.
References

Preclinical activity of datopotamab deruxtecan, a novel TROP2 directed antibody-drug conjugate targeting trophoblast cell-surface antigen 2 (TROP2) in ovarian carcinoma., PMID:38981151

Pannexin 1 Modulates Angiogenic Activities of Human Endothelial Colony-Forming Cells Through IGF-1 Mechanism and Is a Marker of Senescence., PMID:37589139

Evidence of telomere attrition and a potential role for DNA damage in systemic sclerosis., PMID:35086525

Investigating the level of DNA double-strand break in human spermatozoa and its relation to semen characteristics and IVF outcome using phospho-histone H2AX antibody as a biomarker., PMID:31393082

Cytochrome c oxidase deficiency, oxidative stress, possible antioxidant therapy and link to nuclear DNA damage., PMID:29396562

Multiparameter cytometric analysis of complex cellular response., PMID:29220555

Interlaboratory evaluation of a multiplexed high information content in vitro genotoxicity assay., PMID:28370322

γH2AX and p53 responses in TK6 cells discriminate promutagens and nongenotoxicants in the presence of rat liver S9., PMID:27364561

Wee1 Kinase Inhibitor AZD1775 Radiosensitizes Hepatocellular Carcinoma Regardless of TP53 Mutational Status Through Induction of Replication Stress., PMID:26975930

Experimental Adaptation of Rotaviruses to Tumor Cell Lines., PMID:26828934

Genotoxic mode of action predictions from a multiplexed flow cytometric assay and a machine learning approach., PMID:26764165

Cell cycle and apoptosis regulatory proteins, proliferative markers, cell signaling molecules, CD209, and decorin immunoreactivity in low-grade myxofibrosarcoma and myxoma., PMID:25940995

Usefulness of monitoring γ-H2AX and cell cycle arrest in HepG2 cells for estimating genotoxicity using a high-content analysis system., PMID:24980598

SIRT6 protects human endothelial cells from DNA damage, telomere dysfunction, and senescence., PMID:23201774

Follow-up evaluation of radiation-induced DNA damage in CSF disseminated high-grade glioma using phospho-histone H2AX antibody., PMID:21538957

Induction of cell cycle arrest and DNA damage by the HDAC inhibitor panobinostat (LBH589) and the lipid peroxidation end product 4-hydroxynonenal in prostate cancer cells., PMID:21078383

Static electric fields interfere in the viability of cells exposed to ionising radiation., PMID:19399676

Female-specific features of recombinational double-stranded DNA repair in relation to synapsis and telomere dynamics in human oocytes., PMID:15235794

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Phospho-Histone H2A.X (Ser139) Antibody (R1P91) [RHD15008]
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