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Anti-MIF/GIF Antibody (R1P24)

Catalog #:   RHD03402 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: FCM, IP, WB
Accession: P14174
Overview

Catalog No.

RHD03402

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

FCM: 1:20-1:100, IP: 1:20-1:50, WB: 1:1000-1:2000

Target

MIF, L-dopachrome isomerase, L-dopachrome tautomerase, GIF, Macrophage migration inhibitory factor, MMIF, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P14174

Applications

FCM, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1P24

Data Image
  • Western blot
    Western blot analysis of MIF expression in (1) THP-1 cell lysate; (2) Mouse brain lysate.
  • Western blot
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
  • Western blot
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
References

Immunogenomic characteristics and prognostic implications of terminally exhausted CD8+ T cells in colorectal cancers., PMID:40519933

CX3CR1+ Monocytes/Macrophages Promote Regional Immune Injury in Mesangial Proliferative Glomerulonephritis through Crosstalk with Activated Mesangial Cells., PMID:40458609

Multi-omics unveils BCAA metabolism markers L-leucine and HMGCS1 as prognostic marker for immunotherapy efficacy in non-small cell lung cancer., PMID:40457349

MIF regulates T helper 17 cell differentiation by activating the p38 MAPK signaling pathway to drive the pathogenesis of EAP., PMID:40440956

Novel function of macrophage migration inhibitory factor in regulating post-infarct inflammation and the therapeutic significance., PMID:40383291

Inhibition of MIF with an Allosteric Inhibitor Triggers Cell Cycle Arrest in Acute Myeloid Leukemia., PMID:40352549

Pregnant Women Chronically Infected by Toxoplasma gondii with Depressive Disorder: Differential Modulation of Pro-Inflammatory and Anti-Inflammatory Cytokines., PMID:40333139

Single-Cell RNA Sequencing Reveals Macrophage Dynamics During MASH in Leptin-Deficient Rats., PMID:39851524

Exosome-Mediated Lectin Pathway and Resistin-MIF-AA Metabolism Axis Drive Immune Dysfunction in Immune Thrombocytopenia., PMID:39792656

System vaccinology analysis of predictors and mechanisms of antibody response durability to multiple vaccines in humans., PMID:39747435

Integrating In Silico and In Vitro Tools for Optimized Antibody Development-Design of Therapeutic Anti-oxMIF Antibodies., PMID:39727487

Plasma protein dynamics during ipilimumab treatment in metastatic melanoma: associations with tumor response, adverse events and survival., PMID:39703053

Serum cytokines as a biomarker for immune checkpoint inhibitor toxicity in patients with pleural mesothelioma., PMID:39687621

Autofluorescence Virtual Staining System for H&E Histology and Multiplex Immunofluorescence Applied to Immuno-Oncology Biomarkers in Lung Cancer., PMID:39636222

Endothelial progenitor cell-derived conditioned medium mitigates chronic cerebral ischemic injury through macrophage migration inhibitory factor-activated AKT pathway., PMID:39543689

Methionine restriction promotes the polarization of macrophages towards M1 and the immunotherapy effect of PD-L1/PD-1 blockades by inhibiting the secretion of MIF by gastric carcinoma cells., PMID:39541710

The VEGF-Mediated Cytoprotective Ability of MIF-Licensed Mesenchymal Stromal Cells in House Dust Mite-Induced Epithelial Damage., PMID:39502000

CD34 as a potential prognostic indicator for camrelizumab response in advanced non-small-cell lung cancer: insights from digital spatial profiling., PMID:39429466

Targeting of oxidized Macrophage Migration Inhibitory Factor (oxMIF) with antibody ON104 attenuates the severity of glomerulonephritis., PMID:39374239

A Modified Bleaching Method for Multiplex Immunofluorescence Staining of FFPE Tissue Sections., PMID:39370592

Enhancing diagnostic accuracy: Direct immunofluorescence assay as the gold standard for detecting Giardia duodenalis and Cryptosporidium spp. in canine and feline fecal samples., PMID:39358726

Analysis of the role of Dirofilaria repens macrophage migration inhibitory factors in host-parasite interactions., PMID:39318519

Application of Different Staining Methods in the Diagnosis of Pigment-Rich Melanoma., PMID:39315764

Immunologic Crosstalk and Host-Specific Immune Signature Associated with Dengue., PMID:39281909

Therapeutic Opportunities in Breast Cancer by Targeting Macrophage Migration Inhibitory Factor as a Pleiotropic Cytokine., PMID:39246383

Macrophage migration inhibitory factor as a therapeutic target in neuro-oncology: A review., PMID:39233830

Development of a Multiplex Immunofluorescence Assay for Tumor Microenvironment Studies of Human and Murine Merkel Cell Carcinoma., PMID:39182611

Virtual multiplexed immunofluorescence staining from non-antibody-stained fluorescence imaging for gastric cancer prognosis., PMID:39154539

Dynamics of scFv-targeted VAP2 correlating with IL-16, MIF and IL-1Ra in ANCA-associated vasculitis., PMID:39127096

Antibiotic use attenuates response to immune checkpoint blockade in urothelial carcinoma via inhibiting CD74-MIF/COPA: revealing cross-talk between anti-bacterial immunity and ant-itumor immunity., PMID:38995167

Redox-dependent plasticity of oxMIF facilitates its interaction with CD74 and therapeutic antibodies., PMID:38972295

Ion channel profiles of extraocular motoneurons and internuclear neurons in human abducens and trochlear nuclei., PMID:38957435

Detailed Profiling of the Tumor Microenvironment in Ethnic Breast Cancer, Using Tissue Microarrays and Multiplex Immunofluorescence., PMID:38928207

The role of macrophage migration inhibitory factor family and CD74 in the pathogenesis of melanoma., PMID:38884501

Immune profiling of mouse lung adenocarcinoma paraffin tissues using multiplex immunofluorescence panel: a pilot study., PMID:38877529

Risk of Seizure Recurrence Due to Autoimmune Encephalitis With NMDAR, LGI1, CASPR2, and GABABR Antibodies: Implications for Return to Driving., PMID:38838283

GammaGateR: semi-automated marker gating for single-cell multiplexed imaging., PMID:38833684

New-generation technologies for spatial tissue analysis, indispensable tools for deciphering intratumor heterogeneity in the development of antibody-drug conjugates and radio-immunoconjugates for cancer treatment., PMID:38751472

Chordoma cancer stem cell subpopulation characterization may guide targeted immunotherapy approaches to reduce disease recurrence., PMID:38741774

CXCL9 Overexpression Predicts Better HCC Response to Anti-PD-1 Therapy and Promotes N1 Polarization of Neutrophils., PMID:38737384

Infliximab modifies CD74-mediated lymphatic abnormalities and adipose tissue alterations in creeping fat of Crohn's disease., PMID:38713235

Comprehensive multi-omics analysis of resectable locally advanced gastric cancer: Assessing response to neoadjuvant camrelizumab and chemotherapy in a single-center, open-label, single-arm phase II trial., PMID:38685486

MIF-Modulated Spinal Proteins Associated with Persistent Bladder Pain: A Proteomics Study., PMID:38674069

Targeting SIGLEC15 as an emerging immunotherapy for anaplastic thyroid cancer., PMID:38652971

Immunoglobulin-like transcript 2 as an impaired anti-tumor cytotoxicity marker of natural killer cells in patients with hepatocellular carcinoma., PMID:38638429

Single-cell and bulk RNA sequencing highlights the role of M1-like infiltrating macrophages in antibody-mediated rejection after kidney transplantation., PMID:38524599

Granzyme B+ B cells detected by single-cell sequencing are associated with prognosis in patients with intrahepatic cholangiocarcinoma following liver transplantation., PMID:38386050

Multiplex Immunofluorescence Staining Protocol for the Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fixed Paraffin-Embedded Samples., PMID:38319577

Targeting VEGF using Bevacizumab attenuates sepsis-induced liver injury in a mouse model of cecal ligation and puncture., PMID:38313162

Lower serum AMH concentration is correlated with serum IgG1 decreased in the infertile woman: A real-world retrospective study., PMID:38286181

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

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For research use only. Not for human or drug use.

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Anti-MIF/GIF Antibody (R1P24) [RHD03402]
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