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Anti-HSD3B1 Antibody (R1P20)

Catalog #:   RHD02801 Specific References (34) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, WB
Accession: P14060
Overview

Catalog No.

RHD02801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, WB: 1:1000-1:2000

Target

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, HSDB3A, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-HSD I, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3BH, 3-beta-hydroxysteroid 3-dehydrogenase, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, HSD3B1, Steroid Delta-isomerase, Trophoblast antigen FDO161G, Dihydrotestosterone oxidoreductase, Delta-5-3-ketosteroid isomerase

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P14060

Applications

IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1P20

Data Image
  • Western blot
    Western blot analysis of HSD3B1 expression in Human placenta lysate.
  • Western blot
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
  • Western blot
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
References

The Influence of the Germline HSD3B1 Adrenal-Permissive Allele (c.1100 C) on the Somatic Alteration Landscape, the Transcriptome, and Immune Cell Infiltration in Prostate Cancer., PMID:40282446

Microbiota modulates the steroid response to acute immune stress in male mice., PMID:38361932

Interleukins 4 and 13 drive lipid abnormalities in skin cells through regulation of sex steroid hormone synthesis., PMID:34521750

Machine learning gene expression predicting model for ustekinumab response in patients with Crohn's disease., PMID:34469062

Cisatracurium stimulates testosterone synthesis in rat and mouse Leydig cells via nicotinic acetylcholine receptor., PMID:33111502

Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats., PMID:33090725

Three populations of adult Leydig cells in mouse testes revealed by a novel mouse HSD3B1-specific rat monoclonal antibody., PMID:30851938

Development of monoclonal antibodies against the human 3β-hydroxysteroid dehydrogenase/isomerase isozymes., PMID:28863887

Isoform-specific monoclonal antibodies against 3β-hydroxysteroid dehydrogenase/isomerase family provide markers for subclassification of human primary aldosteronism., PMID:24423300

HSD3B1 is a specific trophoblast-associated marker not expressed in a wide spectrum of tumors., PMID:23318910

Fetal testis dysgenesis and compromised Leydig cell function in Tgfbr3 (beta glycan) knockout mice., PMID:19696014

Hypoxia-inducible factor-1alpha is constitutively expressed in murine Leydig cells and regulates 3beta-hydroxysteroid dehydrogenase type 1 promoter activity., PMID:18930903

HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions., PMID:18223326

A case of 3beta-hydroxysteroid dehydrogenase type II (HSD3B2) deficiency picked up by neonatal screening for 21-hydroxylase deficiency: difficulties and delay in etiologic diagnosis., PMID:17496421

YY1 binding within the human HSD3B2 gene intron 1 is required for maximal basal promoter activity: identification of YY1 as the 3beta1-A factor., PMID:15291746

Expression of connexin43 in mouse Leydig, Sertoli, and germinal cells at different stages of postnatal development., PMID:11505367

Stat5-mediated regulation of the human type II 3beta-hydroxysteroid dehydrogenase/delta5-delta4 isomerase gene: activation by prolactin., PMID:10406460

Immunohistochemical study of 3beta-hydroxysteroid dehydrogenase/delta5-delta4 isomerase in the rat cardiovascular system., PMID:9862144

Organization of 3 beta-hydroxysteroid dehydrogenase/isomerase and cytochrome P450scc into a catalytically active molecular complex in bovine adrenocortical mitochondria., PMID:8547175

Ontogenesis of 3 beta-hydroxysteroid dehydrogenase delta 5-delta 4 isomerase in the rat ovary as studied by immunocytochemistry and in situ hybridization., PMID:8439611

Characterization, expression, and immunohistochemical localization of 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase in human skin., PMID:1401999

Immunohistochemical localization of 3 beta-hydroxy-5-ene-steroid dehydrogenase/delta 5----delta 4 isomerase in human placenta and fetal membranes throughout gestation., PMID:1381375

Immunocytochemical localization of 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in human ovary., PMID:1569177

Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes., PMID:1924345

Regulation of adrenal 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase expression and activity by adrenocorticotropin and corticosterone in the rat., PMID:1655393

3 beta-hydroxysteroid dehydrogenase/delta 5----4-isomerase expression in rat and characterization of the testis isoform., PMID:1955079

Corticotropin regulation of 3 beta-hydroxysteroid dehydrogenase/delta 5----4-isomerase in ovine adrenocortical cells: inhibition by transforming growth factor beta., PMID:1851115

Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues., PMID:1985917

Regulation of 3 beta-hydroxysteroid dehydrogenase/delta 5----4-isomerase expression by adrenocorticotropin in bovine adrenocortical cells., PMID:1846095

Ontogeny of 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) in human adrenal gland performed by immunocytochemistry., PMID:2090513

Localization of 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in rat gonads and adrenal glands by immunocytochemistry and in situ hybridization., PMID:2387258

Light microscopic immunocytochemical localization of 3 beta-hydroxy-5-ene-steroid dehydrogenase/delta 5-delta 4-isomerase in the gonads and adrenal glands of the guinea pig., PMID:2351102

3 beta-hydroxysteroid dehydrogenase/isomerase in the fetal zone and neocortex of the human fetal adrenal gland., PMID:2158427

Light and electron microscopic immunocytochemistry on the localization of 3 beta-hydroxysteroid dehydrogenase/isomerase in the bovine adrenal cortical cells., PMID:3163332

Datasheet

Document Download

Anti-HSD3B1 Antibody (R1P20).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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Anti-HSD3B1 Antibody (R1P20) [RHD02801]
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