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Anti-TRIM28/KAP1 Antibody (R1A78)

Catalog #:   RHG46503 Specific References (43) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: FCM, IF, IHC, WB
Accession: Q13263
Overview

Catalog No.

RHG46503

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:5000

Target

E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, KAP1, TF1B, RNF96, TIF1B, TRIM28, TIF1 beta

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13263

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at +4 ℃ short term (1-2 weeks). Store at -20 ℃ 12 months. Store at -80 ℃ long term.

Clone ID

R1A78

Data Image
  • Western blot
    All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
  • Western blot
    Western blot analysis of KAP1 expression in HeLa cell lysate.
References

Single-cell analysis of post-translational modifications identifies immunosuppressive macrophage subtypes in the HBV-positive hepatocellular carcinoma microenvironment., PMID:40455063

TRIM28 functions as SUMO ligase to SUMOylate TRAF6 and regulate NF-κB activation in HBV-replicating cells., PMID:39920527

PRMT5-Mediated ALKBH5 Methylation Promotes Colorectal Cancer Immune Evasion via Increasing CD276 Expression., PMID:39781264

Immune Checkpoint VISTA Negatively Regulates Microglia Glycolysis and Activation via TRIM28-Mediated Ubiquitination of HK2 in Sepsis-Associated Encephalopathy., PMID:39455538

The TRIM28/miR133a/CD47 axis acts as a potential therapeutic target in pancreatic necrosis by impairing efferocytosis., PMID:38872307

The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding., PMID:35563440

A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets., PMID:33082525

Verteporfin Inhibits PD-L1 through Autophagy and the STAT1-IRF1-TRIM28 Signaling Axis, Exerting Antitumor Efficacy., PMID:32265228

Linker histone variant H1t is closely associated with repressed repeat-element chromatin domains in pachytene spermatocytes., PMID:32131873

Anti-TIF1γ antibody and the expression of TIF1γ in idiopathic inflammatory myopathies., PMID:30398003

Autoantibody to transcriptional intermediary factor-1β as a myositis-specific antibody: clinical correlation with clinically amyopathic dermatomyositis or dermatomyositis with mild myopathy., PMID:30120913

Cancer-Germline Antigen Expression Discriminates Clinical Outcome to CTLA-4 Blockade., PMID:29656892

Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non‑small cell lung cancer xenografts., PMID:29115614

TRIM28 epigenetic corepressor is indispensable for stable induced pluripotent stem cell formation., PMID:28759843

Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates., PMID:28498803

Chloroquine triggers Epstein-Barr virus replication through phosphorylation of KAP1/TRIM28 in Burkitt lymphoma cells., PMID:28249048

Metabolic Stress-Induced Phosphorylation of KAP1 Ser473 Blocks Mitochondrial Fusion in Breast Cancer Cells., PMID:27364555

Transcriptional elongation requires DNA break-induced signalling., PMID:26671524

Anti-transcription intermediary factor-1γ/α/β antibody-positive dermatomyositis associated with multiple panniculitis lesions., PMID:26258542

CCAR2/DBC1 is required for Chk2-dependent KAP1 phosphorylation and repair of DNA damage., PMID:26158765

KAP1 promotes proliferation and metastatic progression of breast cancer cells., PMID:25421577

TRIM28 and β-actin identified via nanobody-based reverse proteomics approach as possible human glioblastoma biomarkers., PMID:25419715

Metastasis-associated protein ribosomal RNA processing 1 homolog B (RRP1B) modulates metastasis through regulation of histone methylation., PMID:25092915

STAT3-activated GM-CSFRα translocates to the nucleus and protects CLL cells from apoptosis., PMID:24836891

Kaposi's sarcoma-associated herpesvirus-encoded LANA interacts with host KAP1 to facilitate establishment of viral latency., PMID:24741090

Autoantibodies to transcription intermediary factor TIF1β associated with dermatomyositis., PMID:22513056

KAP1 regulates gene networks controlling mouse B-lymphoid cell differentiation and function., PMID:22452978

A reverse-ELISA for the detection of TRIM28/KAP1 serum autoantibodies in colorectal cancer patients., PMID:22268577

53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair., PMID:20081839

Human IgG antibody profiles differentiate between symptomatic patients with and without colorectal cancer., PMID:19828471

The impact of heterochromatin on DSB repair., PMID:19442252

KRAB zinc-finger proteins localise to novel KAP1-containing foci that are adjacent to PML nuclear bodies., PMID:19258395

Novel breast cancer metastasis-associated proteins., PMID:19086899

The novel protein complex with SMARCAD1/KIAA1122 binds to the vicinity of TSS., PMID:18675275

Phosphorylation at Ser473 regulates heterochromatin protein 1 binding and corepressor function of TIF1beta/KAP1., PMID:18590578

Association of the transcriptional corepressor TIF1beta with heterochromatin protein 1 (HP1): an essential role for progression through differentiation., PMID:15342492

SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins., PMID:11959841

A novel transrepression pathway of c-Myc. Recruitment of a transcriptional corepressor complex to c-Myc by MM-1, a c-Myc-binding protein., PMID:11585818

Transcriptional repression mediated by the KRAB domain of the human C2H2 zinc finger protein Kox1/ZNF10 does not require histone deacetylation., PMID:11405226

A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1., PMID:11013263

Mice lacking the transcriptional corepressor TIF1beta are defective in early postimplantation development., PMID:10851139

Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins., PMID:10549285

KAP-1 corepressor protein interacts and colocalizes with heterochromatic and euchromatic HP1 proteins: a potential role for Krüppel-associated box-zinc finger proteins in heterochromatin-mediated gene silencing., PMID:10330177

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

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For research use only. Not for human or drug use.

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Anti-TRIM28/KAP1 Antibody (R1A78) [RHG46503]
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