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Anti-KDEL (Lys-Asp-Glu-Leu) Antibody (R1A68)

Catalog #:   RHD62501 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: FCM, IF, IHC, WB
Accession: P24390
Overview

Catalog No.

RHD62501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000

Target

KDEL, Lys Asp Glu Leu, KDEL

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P24390

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at +4 ℃ short term (1-2 weeks). Store at -20 ℃ 12 months. Store at -80 ℃ long term.

Clone ID

R1A68

Data Image
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human liver, using KDEL Antibody.
  • Western blot
    Western blot analysis of KDEL expression in HeLa cell lysate.
References

Plant-derived recombinant macromolecular PAP-IgG Fc as a novel prostate cancer vaccine candidate eliciting robust immune responses., PMID:40140219

Comparative Analysis of SPLICS and MCS-DETECT for Detecting Mitochondria-ER Contact Sites (MERCs)., PMID:40115170

CALR-mutated myeloproliferative neoplasms., PMID:39960832

Modification of Fc-fusion protein structures to enhance efficacy of cancer vaccine in plant expression system., PMID:39724301

Plant-derived EpCAM-Fc fusion proteins induce in vivo immune response to produce IgGs inhibiting invasion and migration of colorectal cancer cells., PMID:39630205

Production and characterization of novel Anti-HIV Fc-fusion proteins in plant-based systems: Nicotiana benthamiana & tobacco BY-2 cell suspension., PMID:39142626

Fc engineered anti-virus therapeutic human IgG1 expressed in plants with altered binding to the neonatal Fc receptor., PMID:38528347

Designer Small-Molecule Control System Based on Minocycline-Induced Disruption of Protein-Protein Interaction., PMID:38243811

Enhanced cytotoxicity of a Pseudomonas Exotoxin A based immunotoxin against prostate cancer by addition of the endosomal escape enhancer SO1861., PMID:37484018

Co-transient expression of PSA-Fc and PAP-Fc fusion protein in plant as prostate cancer vaccine candidates and immune responses in mice., PMID:37269373

Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation., PMID:37137223

Plant-derived PAP proteins fused to immunoglobulin A and M Fc domains induce anti-prostate cancer immune response in mice., PMID:37037672

Salvianolic acid A attenuates inflammation-mediated atherosclerosis by suppressing GRP78 secretion of endothelial cells., PMID:36758912

Directing ricin-based immunotoxins with targeting affibodies and KDEL signal peptide to cancer cells effectively induces apoptosis and tumor suppression., PMID:35999603

Enhanced HIV SOSIP Envelope yields in plants through transient co-expression of peptidyl-prolyl isomerase B and calreticulin chaperones and ER targeting., PMID:35705669

Effect of an Endoplasmic Reticulum Retention Signal Tagged to Human Anti-Rabies mAb SO57 on Its Expression in Arabidopsis and Plant Growth., PMID:34711693

Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies)., PMID:34620716

Effect of leaf position and days post-infiltration on transient expression of colorectal cancer vaccine candidate proteins GA733-Fc and GA733-FcK in Nicotiana benthamiana plant., PMID:33868796

Combined immunodeficiency due to a mutation in the γ1 subunit of the coat protein I complex., PMID:33529166

RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells., PMID:33013955

Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System., PMID:32977599

Bispecific Targeting of EGFR and Urokinase Receptor (uPAR) Using Ligand-Targeted Toxins in Solid Tumors., PMID:32630411

Expression and In Vitro Function of Anti-Breast Cancer Llama-Based Single Domain Antibody VHH Expressed in Tobacco Plants., PMID:32079309

Improvement of Intracellular Traffic System by Overexpression of KDEL Receptor 1 in Antibody-Producing CHO Cells., PMID:32073237

Synthetic antibody discovery against native antigens by CRISPR/Cas9-library generation and endoplasmic reticulum screening., PMID:32020276

Repeated Oral Administration of a KDEL-tagged Recombinant Cholera Toxin B Subunit Effectively Mitigates DSS Colitis Despite a Robust Immunogenic Response., PMID:31756977

Expression and in vitro function of anti-cancer mAbs in transgenic Arabidopsis thaliana., PMID:31234954

ATPase activity of human binding immunoglobulin protein (BiP) variants is enhanced by signal sequence and physiological concentrations of Mn2., PMID:31033254

Transient expression of etanercept therapeutic protein in tobacco (Nicotiana tabacum L.)., PMID:30825567

Plant-derived secretory component forms secretory IgA with shiga toxin 1-specific dimeric IgA produced by mouse cells and whole plants., PMID:30506369

Human colorectal cancer antigen GA733-2-Fc fused to endoplasmic reticulum retention motif KDEL enhances its immunotherapeutic effects., PMID:30249898

Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response., PMID:30248125

Data on leukocyte PDZK1 deficiency affecting macrophage apoptosis but not monocyte recruitment, cell proliferation, macrophage abundance or ER stress in atherosclerotic plaques of LDLR deficient mice., PMID:30246067

Construction and characterization of the recombinant immunotoxin RTA-4D5-KDEL targeting HER2/neu-positive cancer cells and locating the endoplasmic reticulum., PMID:30141083

Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression., PMID:29441088

In Vitro Evaluation of Vegf-Pseudomonas Exotoxin: A Conjugated on Tumor Cells., PMID:29226113

An anti-amoebic vaccine: generation of the recombinant antigen LC3 from Entamoeba histolytica linked to mutated exotoxin A (PEΔIII) via the Pichia pastoris system., PMID:28470625

Intrabody targeting vascular endothelial growth factor receptor-2 mediates downregulation of surface localization., PMID:27982020

FAD-linked Presenilin-1 V97L mutation impede tranport regulation and intracellular Ca(2+) homeostasis under ER stress., PMID:26884997

A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles., PMID:26861384

Heterologous Expression of Hepatitis C Virus Core Protein in Oil Seeds of Brassica napus L., PMID:26855744

Endoplasmic Reticulum (ER)-Targeted, Galectin-Mediated Retrograde Transport by Using a HaloTag Carrier Protein., PMID:26773807

Transgenic Production of an Anti HIV Antibody in the Barley Endosperm., PMID:26461955

Specific in vivo knockdown of protein function by intrabodies., PMID:26252565

Tobacco seeds as efficient production platform for a biologically active anti-HBsAg monoclonal antibody., PMID:26109093

Malaria DNA vaccine gp96NTD-CSP elicits both CSP-specific antibody and CD8(+) T cell response., PMID:25786609

Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity., PMID:25713356

Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris., PMID:25641071

Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications., PMID:25598788

Functional knock down of VCAM1 in mice mediated by endoplasmatic reticulum retained intrabodies., PMID:25484057

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-KDEL (Lys-Asp-Glu-Leu) Antibody (R1A68) [RHD62501]
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