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Anti-Human FGL1/HFREP1 Monoclonal Antibody (1A491)

Catalog #:   MHG17901 Specific References (26) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: ELISA, FCM, IF, IHC
Accession: Q08830
Overview

Catalog No.

MHG17901

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Immunogen

Recombinant Human FGL1/HFREP1 (Leu23-Ile312).

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q08830

Applications

ELISA, FCM, IF, IHC

Form

Liquid

Storage buffer

PBS with 0.05% sodium azide

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.

Alternative Names

Liver fibrinogen-related protein 1, HFREP-1, HP-041, Hepatocyte-derived fibrinogen-related protein 1, HPS, FGL1, LFIRE-1, Hepassocin, Fibrinogen-like protein 1, HFREP1

Clone ID

1A491

Data Image
  • ELISA
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
  • FCM
    Flow cytometric analysis of Hela cells using FGL1 mouse mAb (green) and negative control (red).
  • IMMUNOFLUORESCENCE
    Immunofluorescence analysis of Hela cells using FGL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • WESTERN BLOT
    Recombinant lysates were subjected to SDS PAGE followed by western blot with FGL1/HFREP1 antibody (MHG17901) at 1 μg/ml.

    Lane 1: Recombinant human FGL1 Protein

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
  • WESTERN BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with FGL1/HFREP1 antibody (MHG17901) at 1 μg/ml.

    Lane 1: Non-transfected HEK293 cell lysate
    Lane 2: FGL1 transfected HEK293 cell lysate

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 55 kDa
    Observed MW: 55 kDa
  • IMMUNOHISTOCHEMISTRY
    Immunohistochemical analysis of paraffin-embedded rectal cancer tissues stained for FGL1/HFREP1 with MHG17901.
  • IMMUNOHISTOCHEMISTRY
    Immunohistochemical analysis of paraffin-embedded lung cancer tissues stained for FGL1/HFREP1 with MHG17901.
References

Exploring new frontiers in LAG-3 biology and therapeutics., PMID:40514283

Research advances in FGL1/LAG3 for cancer diagnosis and treatment: From basics to clinical practice., PMID:40317138

Design of NanoBiT-Nanobody-based FGL1 biosensors for early assisted diagnosis of esophageal cancer., PMID:40138964

Ligand-induced ubiquitination unleashes LAG3 immune checkpoint function by hindering membrane sequestration of signaling motifs., PMID:40101708

FGL1 facilitates rather than suppresses anticancer immunity against microsatellite instable gastric cancer., PMID:39672971

A novel LAG3 neutralizing antibody improves cancer immunotherapy by dual inhibition of MHC-II and FGL1 ligand binding., PMID:38776682

The immune checkpoint receptor LAG3: Structure, function, and target for cancer immunotherapy., PMID:38556085

Development of LAG-3/FGL1 blocking peptide and combination with radiotherapy for cancer immunotherapy., PMID:38486998

Structural insights reveal interplay between LAG-3 homodimerization, ligand binding, and function., PMID:38483996

[Screening and activity analysis of nanobodies against lymphocyte activation gene-3(LAG-3)]., PMID:37980554

FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation., PMID:37938970

FGL1-LAG3 axis impairs IL-10-Producing regulatory T cells associated with Systemic lupus erythematosus disease activity., PMID:37916085

The ligand-dependent suppression of TCR signaling by the immune checkpoint receptor LAG3 depends on the cytoplasmic RRFSALE motif., PMID:37788323

LAG-3 as a Potent Target for Novel Anticancer Therapies of a Wide Range of Tumors., PMID:36077354

The promising immune checkpoint LAG-3 in cancer immunotherapy: from basic research to clinical application., PMID:35958563

LAG3 ectodomain structure reveals functional interfaces for ligand and antibody recognition., PMID:35761082

Immune Checkpoint LAG3 and Its Ligand FGL1 in Cancer., PMID:35111155

Fibrinogen-like protein 1 (FGL1): the next immune checkpoint target., PMID:34526102

A Double-Antibody Sandwich ELISA for Sensitive and Specific Detection of Swine Fibrinogen-Like Protein 1., PMID:33968077

Development of a nanobody-based immunoassay for the sensitive detection of fibrinogen-like protein 1., PMID:33633363

Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3., PMID:30580966

Hepassocin regulates cell proliferation of the human hepatic cells L02 and hepatocarcinoma cells through different mechanisms., PMID:21618590

A protein chip approach for high-throughput antigen identification and characterization., PMID:17549792

Evaluation of progesterone content in saliva using magnetic particle-based immuno supported liquid membrane assay (m-ISLMA)., PMID:16473507

Genetic variations in humans associated with differences in the course of hepatitis C., PMID:15063762

Isolation and characterization of a novel liver-specific gene, hepassocin, upregulated during liver regeneration., PMID:11004478

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-Human FGL1/HFREP1 Monoclonal Antibody (1A491) [MHG17901]
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