Anti-Human FGL1/HFREP1 Monoclonal Antibody (1A491)

Catalog #: MHG17901 Specific References (0) DATASHEET

Host species: Mouse

Isotype: IgG2a

Applications: ELISA, FCM, IF, IHC

Accession: Q08830

Overview

Catalog No.

MHG17901

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Immunogen

Recombinant Human FGL1/HFREP1 (Leu23-Ile312).

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q08830

Applications

ELISA, FCM, IF, IHC

Form

Liquid

Storage buffer

PBS with 0.05% sodium azide

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.

Alternative Names

Liver fibrinogen-related protein 1, HFREP-1, HP-041, Hepatocyte-derived fibrinogen-related protein 1, HPS, FGL1, LFIRE-1, Hepassocin, Fibrinogen-like protein 1, HFREP1

Clone ID

1A491

Data Image

ELISA

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
FCM

Flow cytometric analysis of Hela cells using FGL1 mouse mAb (green) and negative control (red).
IMMUNOFLUORESCENCE

Immunofluorescence analysis of Hela cells using FGL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
WESTERN BLOT

Recombinant lysates were subjected to SDS PAGE followed by western blot with FGL1/HFREP1 antibody (MHG17901) at 1 μg/ml.

Lane 1: Recombinant human FGL1 Protein

Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

Predict MW: 37 kDa
Observed MW: 37 kDa
WESTERN BLOT

Various lysates were subjected to SDS PAGE followed by western blot with FGL1/HFREP1 antibody (MHG17901) at 1 μg/ml.

Lane 1: Non-transfected HEK293 cell lysate
Lane 2: FGL1 transfected HEK293 cell lysate

Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

Predict MW: 55 kDa
Observed MW: 55 kDa
IMMUNOHISTOCHEMISTRY

Immunohistochemical analysis of paraffin-embedded rectal cancer tissues stained for FGL1/HFREP1 with MHG17901.
IMMUNOHISTOCHEMISTRY

Immunohistochemical analysis of paraffin-embedded lung cancer tissues stained for FGL1/HFREP1 with MHG17901.

References

Datasheet

Document Download

Anti-Human FGL1/HFREP1 Monoclonal Antibody (1A491).pdf

$ 220

Product specifications

50 μg 220 100 μg 350

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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