Catalog No.
AHJ71601
Biological activity
Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells. When 5 x 10^4 cells/well are added to recombinant human SEMA5B (Cat #: AHJ71601) coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 ℃. The ED50 for this effect is 0.7‑2.8 μg/mL.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
Val78-Thr834
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9P283
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
SEMAG, Semaphorin-5B, SEMA5B, KIAA1445
Demystifying the impact of prenatal tobacco exposure on the placental immune microenvironment: Avoiding the tragedy of mending the fold after death., PMID:37700485
Axon Fasciculation, Mediated by Transmembrane Semaphorins, Is Critical for the Establishment of Segmental Specificity of Corticospinal Circuits., PMID:37344234
Genes associated with diagnosis and prognosis of Burkitt lymphoma., PMID:36354023
Combining bioinformatics, network pharmacology and artificial intelligence to predict the mechanism of celastrol in the treatment of type 2 diabetes., PMID:36339449
Identification of 8-gene risk prediction signature associated with NOTCH1 in stomach adenocarcinoma based on bioinformatics analysis., PMID:36092350
Remote ischemic preconditioning causes transient cell cycle arrest and renal protection by a NF-κB-dependent Sema5B pathway., PMID:35727636
Magnesium-Encapsulated Injectable Hydrogel and 3D-Engineered Polycaprolactone Conduit Facilitate Peripheral Nerve Regeneration., PMID:35652188
Prognostic and Diagnostic Values of Semaphorin 5B and Its Correlation With Tumor-Infiltrating Immune Cells in Kidney Renal Clear-Cell Carcinoma., PMID:35480320
MicroRNA-210 regulates the metabolic and inflammatory status of primary human astrocytes., PMID:34991629
Transcriptomic profile of Pea3 family members reveal regulatory codes for axon outgrowth and neuronal connection specificity., PMID:33097800
Pax6 modulates intra-retinal axon guidance and fasciculation of retinal ganglion cells during retinogenesis., PMID:32999322
PRDM16 suppresses HIF-targeted gene expression in kidney cancer., PMID:32251515
Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement during Development., PMID:31209173
Prioritizing candidate genes for fertility in dairy cows using gene-based analysis, functional annotation and differential gene expression., PMID:30935378
Assays to Examine Transmembrane Semaphorin Function In Vitro., PMID:27787856
Association Between Three SNPs and Thromboangiitis Obliterans in Xinjiang Uyghur Population., PMID:26829209
Methyl-CpG Binding Protein 2 (Mecp2) Regulates Sensory Function Through Sema5b and Robo2., PMID:26733807
Driver Gene and Novel Mutations in Asbestos-Exposed Lung Adenocarcinoma and Malignant Mesothelioma Detected by Exome Sequencing., PMID:26463840
Semaphorin 5B is a repellent cue for sensory afferents projecting into the developing spinal cord., PMID:24718987
Expansion of the piriform cortex contributes to corticothalamic pathfinding defects in Gli3 conditional mutants., PMID:24014668
Sema6B, Sema6C, and Sema6D expression and function during mammalian retinal development., PMID:23646199
Transmembrane semaphorin5B is proteolytically processed into a repulsive neural guidance cue., PMID:22817385
Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function., PMID:21835343
Semaphorin 5B mediates synapse elimination in hippocampal neurons., PMID:19463192
Identification and localization of novel genes preferentially expressed in human kidney glomerulus., PMID:19019172
Combined activation of calpain and calcineurin during ligand-induced growth cone collapse., PMID:17826176
Genome-wide gene expression profiles of clear cell renal cell carcinoma: identification of molecular targets for treatment of renal cell carcinoma., PMID:16964377
For research use only. Not for human or drug use.
