Catalog No.
AHN01001
Biological activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in human embryonic kidney cells (HEK293). The ED50 for this effect is 0.1-0.6 μg/mL in the presence of 0.5 μg/mL of recombinant human Wnt-3a.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
Ser81-Thr451 (Cys330Ser)
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Q6P988
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
NOTUM, hNOTUM, Palmitoleoyl-protein carboxylesterase NOTUM
Bioactive components of Jiedu Sangen decoction against colorectal cancer: A novel and comprehensive research strategy for natural drug development., PMID:40279966
Developmental onset of planarian whole-body regeneration depends on axis reset., PMID:40239657
New Qiangguyin activates Wnt/β-catenin pathway by down-regulating Notum to improve osteoporosis in rats., PMID:40210176
Advantages and Limitations of Photoconvertible Probes to Study Subcellular Dynamics in Epithelial Cells., PMID:40098335
map3k1 suppresses terminal differentiation of migratory eye progenitors in planarian regeneration., PMID:40096024
Integration of osimertinib-targeted EGFR gene-associated differential gene expression in constructing a prognostic model for lung adenocarcinoma., PMID:39661202
Therapeutic targeting of Wnt antagonists by small molecules for treatment of osteoporosis., PMID:39447984
USP10 drives cancer stemness and enables super-competitor signalling in colorectal cancer., PMID:39443725
Upregulation of Fatty Acid Synthase Increases Activity of β-Catenin and Expression of NOTUM to Enhance Stem-like Properties of Colorectal Cancer Cells., PMID:39404424
NOTUM-mediated WNT silencing drives extravillous trophoblast cell lineage development., PMID:39325428
Brainstem transcriptomic changes in male Wistar rats after acute stress, comparing the use of duplex specific nuclease (DSN)., PMID:39300279
Efficient CRISPR/Cas9-mediated ebony gene editing in the greater wax moth Galleria mellonella., PMID:39121464
Proteome- and Transcriptome-Wide Genetic Analysis Identifies Biological Pathways and Candidate Drug Targets for Preeclampsia., PMID:39119725
New-QiangGuYin-Containing Serum Inhibits Osteoclast-Derived Exosome Secretion and Down-Regulates Notum to Promote Osteoblast Differentiation., PMID:38935529
Increasing GSH-Px Activity and Activating Wnt Pathway Promote Fine Wool Growth in FGF5-Edited Sheep., PMID:38891117
Notum regulates the cusp and root patterns in mouse molar., PMID:38871845
Genetic insights and therapeutic potential for colorectal cancer: mutation analysis of KRAS gene and efficacy of Oleuropein-conjugated iron oxide nanoparticles., PMID:38837069
Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts., PMID:38786073
After wounding, a G-protein coupled receptor promotes the restoration of tension in epithelial cells., PMID:38536445
Live imaging basement membrane assembly under the pupal notum epithelium., PMID:38525127
NOTUM-MEDIATED WNT SILENCING DRIVES EXTRAVILLOUS TROPHOBLAST CELL LINEAGE DEVELOPMENT., PMID:38405745
5-Aza-2'-Deoxycytidine Ameliorates Choroidal Neovascularization by Inhibiting the Wnt/β-Catenin Signaling Pathway., PMID:38345554
ESCRT-III-dependent adhesive and mechanical changes are triggered by a mechanism detecting alteration of septate junction integrity in Drosophila epithelial cells., PMID:38305711
NOTUM plays a bidirectionally modulatory role in the odontoblastic differentiation of human stem cells from the apical papilla through the WNT/β-catenin signaling pathway., PMID:38278124
Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens., PMID:38154460
On-demand chlorine dioxide solution enhances odontoblast differentiation through desulfation of cell surface heparan sulfate proteoglycan and subsequent activation of canonical Wnt signaling., PMID:37954207
A whole genome scan reveals distinct features of selection in Zhaotong cattle of Yunnan province., PMID:37796667
Notum enhances gastric cancer stem-like cell properties through upregulation of Sox2 by PI3K/AKT signaling pathway., PMID:37749430
Identification and verification of plasma protein biomarkers that accurately identify an ectopic pregnancy., PMID:37715129
APC and P53 mutations synergise to create a therapeutic vulnerability to NOTUM inhibition in advanced colorectal cancer., PMID:37591698
Associations of circulating proteins with lipoprotein profiles: proteomic analyses from the OmniHeart randomized trial and the Atherosclerosis Risk in Communities (ARIC) Study., PMID:37400771
After wounding, a G-protein coupled receptor promotes the restoration of tension in epithelial cells., PMID:37398151
Codon-optimized TDP-43 mediates neurodegeneration in a Drosophila model of ALS/FTLD., PMID:36968586
The logistics of Wnt production and delivery., PMID:36967191
Rational Construction of a Novel Bioluminescent Substrate for Sensing the Tumor-Associated Hydrolase Notum., PMID:36962078
Wnt/β-catenin signalling is required for pole-specific chromatin remodeling during planarian regeneration., PMID:36653403
Human ERG oncoprotein represses a Drosophila LIM domain binding protein-coding gene Chip., PMID:36595681
Functional regulation of Wnt protein through post-translational modifications., PMID:36484635
The expression of NOTUM in replantation of severed fingers may be an important treatment factor., PMID:36465015
The multifaceted role of GCM1 during trophoblast differentiation in the human placenta., PMID:36442132
Applications of Immunomagnetic Reduction Technology as a Biosensor in Therapeutic Evaluation of Chinese Herbal Medicine in Tauopathy Alleviation of an AD Drosophila Model., PMID:36291020
Notum leads to potential pro-survival of OSCC through crosstalk between Shh and Wnt/β-catenin signaling via p-GSK3β., PMID:36280040
Tissue flow regulates planar cell polarity independently of the Frizzled core pathway., PMID:36130497
The CHAMP-study: the CHemopreventive effect of lithium in familial AdenoMatous Polyposis; study protocol of a phase II trial., PMID:35962368
Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion., PMID:35752632
The Tbx6 Transcription Factor Dorsocross Mediates Dpp Signaling to Regulate Drosophila Thorax Closure., PMID:35562934
Design of a Potent, Selective, and Brain-Penetrant Inhibitor of Wnt-Deactivating Enzyme Notum by Optimization of a Crystallographic Fragment Hit., PMID:35536179
Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model., PMID:35463060
Src acts with WNT/FGFRL signaling to pattern the planarian anteroposterior axis., PMID:35297964
Analysis of transcript-wide profile regulated by microsatellite instability of colorectal cancer., PMID:35280417
For research use only. Not for human or drug use.
