Catalog No.
AHB70501
Biological activity
Measured by its binding ability in a functional ELISA. When recombinant Rat UNC5H2 is immobilized at 5 μg/mL, recombinant human NTN1/Netrin-1 (Cat #: AHB70501) binds with an apparent Kd <1 nM. Measured in a cell proliferation assay using rat schwannoma cells (RT4‑D6P2T). The ED50 for this effect is 0.5-2.0 μg/mL.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
Val22-Ala604
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
O95631
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Netrin-1, NTN1, Epididymis tissue protein Li 131P, NTN1L
Sonic hedgehog medulloblastomas are dependent on Netrin-1 for survival., PMID:40461501
The role of netrin-1 in the diagnosis and prognosis of bladder pain syndrome /interstitial cystitis: a comparative study., PMID:40459754
Regulatory role of neuronal guidance proteins in spinal cord injury., PMID:40364635
NTN-1 attenuates amyloid-β-mediated microglial neuroinflammation and memory impairment via the NF-κB pathway and NLRP3 inflammasome in a rat model of Alzheimer's disease., PMID:40357234
Transient renal tubular injury among children and adolescents during diabetic ketoacidosis: severity, renal perfusion, and urinary netrin- 1 interplay., PMID:40332546
Altered renal vascular patterning reduces ischemic kidney injury and limits age-associated vascular loss., PMID:40331791
MAGI1 attenuates osteoarthritis by regulating osteoclast fusion in subchondral bone through the RhoA-ROCK1 signaling pathway., PMID:40322041
Novel miRNAs, miR-937-3p, miR-4536-3p, and miR-4650-5p, can Modulate Neuronal Differentiation via the Wnt/MAPK Pathway in SH-SY5Y Cells., PMID:40316877
Slit-Robo signaling supports motor neuron avoidance of the spinal cord midline through DCC antagonism and other mechanisms., PMID:40276653
Gram-negative bacteria activate cellular pathways in plaque microenvironment; Systems biology approach., PMID:40275124
Netrin-1 and B-cell maturation antigen expression in a large cohort of 361 lymphomas: sensitive and specific staining in plasmablastic lymphomas, and therapeutic perspectives., PMID:40235169
In silico detection of dysregulated genes and molecular pathways in Alzheimer's disease as basis for food restoring approach., PMID:40212376
The usefulness of urinary netrin-1 determination as an early marker of acute kidney injury in children after cardiac surgery., PMID:40190310
Novel insights into the regulation of neuron migration by axon guidance proteins., PMID:40184989
Butylphthalide protects cerebral infarction in a rat model by regulating netrin-1/deleted in colorectal cancer/vascular endothelial growth factor axis., PMID:40177830
Exploring The Role of Serum Netrin 1 Levels in Diabetic Foot Syndrome And Uncomplicated Type 2 Diabetes Mellitus: A Comparative Analysis., PMID:40176590
Coro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis., PMID:40166342
The contribution of de novo coding mutations to meningomyelocele., PMID:40140573
Systemic Lupus Erythematosus Stimulates Chondrocyte Pyroptosis to Aggravate Arthritis via Suppression of NRF-2/KEAP-1 and NF-κB Pathway., PMID:40129871
A stable NTN1 fluorescent reporter chicken reveals cell specific molecular signatures during optic fissure closure., PMID:40128351
Lentivirus-mediated overexpression of netrin-1/DCC co-expression promotes axonal regeneration and functional recovery in spinal cord injury via the inhibition of the NgR1-RhoA-ROCK signaling pathway., PMID:40092657
Netrin-1 binding to UNC5b improves post-stroke neuronal ferroptosis via AMPK-BACH1 pathway., PMID:40086580
Amphetamine in Adolescence Induces a Sex-Specific Mesolimbic Dopamine Phenotype in the Adult Prefrontal Cortex., PMID:40060609
Modulation of netrin-1/DCC signaling pathway by Jiawei Kongsheng Zhenzhong Pill improves synaptic structural plasticity in PSD rats., PMID:40058944
Microbiota and Inflammatory Markers: A Review of Their Interplay, Clinical Implications, and Metabolic Disorders., PMID:40004236
Determination of Urine Netrin-1 and Beta-Hydroxy Butyrate Levels in Diabetic Ketoacidosis Cases: a Preliminary Report., PMID:39967526
mPFC DCC coupling with CaMKII+ neuronal excitation participates in behavioral despair in male mice., PMID:39952936
Anticancer (cytotoxic, anticlonogenic, antimetastatic, immunomodulatory actions) properties of 3,5-dibromosalicylaldehyde against glioblastoma cells and DFT analyses (FT-IR, Raman, NMR, UV) as well as a molecular docking study., PMID:39924847
TRIM9 Controls Growth Cone Responses to Netrin Through DCC and UNC5C., PMID:39871643
Endothelial lipase-binding peptides similar to netrin-1 inhibit hepatitis B virus infection., PMID:39865491
Identification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis., PMID:39863862
Comprehensive pan-cancer analysis reveals NTN1 as an immune infiltrate risk factor and its potential prognostic value in SKCM., PMID:39863609
Short- and long-range roles of UNC-6/Netrin in dorsal-ventral axon guidance in vivo in Caenorhabditis elegans., PMID:39823521
Predictive Value of Netrin-1 Expression and Ultrasonic Blood Flow in Cervical Intraepithelial Neoplasia Severity., PMID:39802920
Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit., PMID:39774925
Mossy Fiber Sprouting in Temporal Lobe Epilepsy: The Impact of Netrin-1, DCC, and Gene Expression Changes., PMID:39767775
Bafilomycin A1 mitigates subchondral bone degeneration and pain in TMJOA rats., PMID:39756167
The regulatory role of the Netrin-1/UNC5H3 pathway in neuronal pyroptosis after stroke., PMID:39740525
Netrin-1: Key insights in neural development and disorders., PMID:39719818
Hepatocellular carcinoma hosts cholinergic neural cells and tumoral hepatocytes harboring targetable muscarinic receptors., PMID:39717507
Early Detection of Diabetic Nephropathy Based on Urinary and Serum Biomarkers: An Updated Systematic Review., PMID:39717256
Plasma Transcriptome Profile Associated with Chronic Kidney Disease Progression., PMID:39694024
Interplay between Netrin-1 and Norrin controls arteriovenous zonation of blood-retina barrier integrity., PMID:39693351
A Novel De Novo Missense Variant in Netrin-1 (NTN1) Associated With Chorioretinal Coloboma, Sensorineural Hearing Loss and Polydactyly., PMID:39648562
Electroacupuncture improves cognitive function in high-fat diet/streptozocin-induced type 2 diabetic mice by inhibiting autophagy-related ferroptosis., PMID:39647901
Quercetin Alleviates Diabetic Peripheral Neuropathy by Regulating Axon Guidance Factors and Inhibiting the Rho/ROCK Pathway in vivo and in vitro., PMID:39582785
Molecular diversity and migration of GABAergic neurons in the developing ventral midbrain., PMID:39569362
Altered renal vascular patterning reduces ischemic kidney injury and limits vascular loss associated with aging., PMID:39553980
Netrin1 patterns the dorsal spinal cord through modulation of Bmp signaling., PMID:39547237
Pinocembrin alleviates LPS-induced depressive-like behavior in mice via the NLRP3/DCC signaling pathway., PMID:39476759
For research use only. Not for human or drug use.
