Catalog No.
YZZ06401
Expression system
E. coli
Species
Malus domestica (Apple) (Pyrus malus)
Protein length
Lys90-Ser593
Predicted molecular weight
57.54 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P43309
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Polyphenol oxidase, chloroplastic, Catechol oxidase, PPO
Genome-Wide Identification of the PR-1 Gene Family in Pyrus betulaefolia Bunge and Its Expression Analysis Under Fire Blight Stress., PMID:40507885
Oxygenase Ppo-Regulated Moldy Volatiles Affect Growth, Pathogenicity and Patulin Biosynthesis of Penicillium expansum Through G Protein Signaling., PMID:39728322
Cold plasma and honey synergistically inhibit polyphenol oxidase to enhance fresh-cut apple preservation., PMID:39708505
Cold atmospheric plasma inactivation of polyphenol oxidase: Focus on the protective and boosting effect of mono- and disaccharides., PMID:39674814
Expression of the polyphenol oxidase gene MdPPO7 is modulated by MdWRKY3 to regulate browning in sliced apple fruit., PMID:39535880
Insights into inhibitory action and interaction of bisdemethoxycurcumin on tyrosinase: Spectroscopic and docking analysis., PMID:39423987
The elicitation effects of diode and He-Ne laser irradiations on the alleviation of nutrient-deficiency induced damage in anthocyanin-producing red-fleshed apple cell suspension., PMID:39259817
Purification and characterization of novel isoforms of the polyphenol oxidase from Malus domestica fruit pulp., PMID:37624797
Application of Whey Protein-Based Emulsion Coating Treatment in Fresh-Cut Apple Preservation., PMID:36981067
Purification and Characterization of a Dark Red Skin Related Dimeric Polyphenol Oxidase from Huaniu Apples., PMID:35741987
Multi-Omics Landscape of DNA Methylation Regulates Browning in "Fuji" Apple., PMID:35198585
Comparative physiological and transcriptomic analysis reveal MdWRKY75 associated with sucrose accumulation in postharvest 'Honeycrisp' apples with bitter pit., PMID:35176994
Transcriptomics Reveals the ERF2-bHLH2-CML5 Module Responses to H2S and ROS in Postharvest Calcium Deficiency Apples., PMID:34884817
Relationship between Phenolic Compounds, Antioxidant Properties, and the Allergenic Protein Mal d 1 in Different Selenium-Biofortified Apple Cultivars (Malus domestica)., PMID:33946582
Biocontrol efficiency of Meyerozyma guilliermondii Y-1 against apple postharvest decay caused by Botryosphaeria dothidea and the possible mechanisms of action., PMID:33221041
Transcriptional and physiological analyses of reduced density in apple provide insight into the regulation involved in photosynthesis., PMID:33044972
Inhibitory Effect of Azamacrocyclic Ligands on Polyphenol Oxidase in Model and Food Systems., PMID:32609498
Influence of the functionalisation of mesoporous silica material UVM-7 on polyphenol oxidase enzyme capture and enzymatic browning., PMID:31806389
Structural changes of a protein extract from apple with polyphenoloxidase activity obtained by cationic reversed micellar extraction induced by high-pressure carbon dioxide and thermosonication., PMID:31551453
Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols., PMID:31022487
New Method for Accurate Determination of Polyphenol Oxidase Activity Based on Reduction in SERS Intensity of Catechol., PMID:30209938
Aggregation and Conformational Changes in Native and Thermally Treated Polyphenol Oxidase From Apple Juice (Malus domestica)., PMID:29922647
Browning in apples: Exploring the biochemical basis of an easily-observable phenotype., PMID:28843018
Three recombinantly expressed apple tyrosinases suggest the amino acids responsible for mono- versus diphenolase activity in plant polyphenol oxidases., PMID:28821733
In crystallo activity tests with latent apple tyrosinase and two mutants reveal the importance of the mutated sites for polyphenol oxidase activity., PMID:28777094
Unraveling the inhibition mechanism of cyanidin-3-sophoroside on polyphenol oxidase and its effect on enzymatic browning of apples., PMID:28274409
Application of mesoporous silica materials for the immobilization of polyphenol oxidase., PMID:27664646
Extraction and Separation of Active Ingredients in Schisandra chinensis (Turcz.) Baill and the Study of their Antifungal Effects., PMID:27152614
Salicylic acid confers enhanced resistance to Glomerella leaf spot in apple., PMID:27139585
Interaction of polyphenol oxidase of Solanum tuberosum with β-cyclodextrin: Process details and applications., PMID:26187193
Induction of resistance mediated by an attenuated strain of Valsa mali var. mali using pathogen-apple callus interaction system., PMID:25054166
Effect of halide salts on development of surface browning on fresh-cut 'Granny Smith' (Malus × domestica Borkh) apple slices during storage at low temperature., PMID:24898689
Polyphenol oxidase activity and antioxidant properties of Yomra apple (Malus communis L.) from Turkey., PMID:24246090
Extraction and quantitation of coumarin from cinnamon and its effect on enzymatic browning in fresh apple juice: a bioinformatics approach to illuminate its antibrowning activity., PMID:23683299
The impact of catechin and epicatechin, total phenols and PPO activity on the Mal d 1 content in apple fruit., PMID:23578620
Modelling the interactions between free phenols, L-ascorbic acid, apple polyphenoloxidase and oxygen during a thermal treatment., PMID:23411245
Effect of trehalose on the biocontrol efficacy of Pichia caribbica against post-harvest grey mould and blue mould decay of apples., PMID:23325746
Effects of mouldy core and core rot on physiological and biochemical responses of apple fruit., PMID:21681764
Effects of argon enriched low-oxygen atmospheres and of high-oxygen atmospheres on the kinetics of polyphenoloxidase (PPO)., PMID:21535678
Development of an ingredient containing apple peel, as a source of polyphenols and dietary fiber., PMID:20722929
Distinguishing clonal apple rootstocks by isozymes banding patterns., PMID:11906109
Fast apple (Malus x domestica) and tobacco (Nicotiana tobacum) leaf polyphenol oxidase activity assay for screening transgenic plants., PMID:11141262
Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees., PMID:10385997
Unfolding and refolding of active apple polyphenol oxidase., PMID:9842726
Cloning genomic DNA encoding apple polyphenol oxidase and comparison of the gene product in Escherichia coli and in apple., PMID:9532795
Subcellular location of polyphenol oxidase in apples., PMID:9339550
New spectrophotometric assay for polyphenol oxidase activity., PMID:8297016
For research use only. Not for human or drug use.
