Recombinant PDCoV Nsp5/Mpro/3CL Protein, N-His

Catalog No.

YVV43501

Expression system

E. coli

Species

Porcine deltacoronavirus

Protein length

Ala2509-Gln2815

Predicted molecular weight

36.85 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

UVJ47558.1

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

3C-like proteinase, 3CL-PRO, Main protease, Mpro, Non-structural protein 5, nsp5, ORF1ab polyprotein

Cleavage of the selective autophagy receptor NBR1 by the PDCoV main protease NSP5 impairs autophagic degradation of the viral envelope protein., PMID:40047225

Porcine deltacoronavirus nsp5 antagonizes type I interferon signaling by cleaving IFIT3., PMID:38289117

Cleavage of HDAC6 to dampen its antiviral activity by nsp5 is a common strategy of swine enteric coronaviruses., PMID:38289103

Broad antagonism of coronaviruses nsp5 to evade the host antiviral responses by cleaving POLDIP3., PMID:37801439

Porcine Deltacoronavirus Infection Cleaves HDAC2 to Attenuate Its Antiviral Activity., PMID:35916536

Coronaviruses Nsp5 Antagonizes Porcine Gasdermin D-Mediated Pyroptosis by Cleaving Pore-Forming p30 Fragment., PMID:35012343

Porcine Deltacoronavirus nsp5 Cleaves DCP1A To Decrease Its Antiviral Activity., PMID:32461317

Porcine deltacoronavirus nucleocapsid protein antagonizes IFN-β production by impairing dsRNA and PACT binding to RIG-I., PMID:31129785

Porcine Deltacoronavirus nsp5 Antagonizes Type I Interferon Signaling by Cleaving STAT2., PMID:28250121

Porcine deltacoronavirus nsp5 inhibits interferon-β production through the cleavage of NEMO., PMID:27984784

Document Download

Recombinant PDCoV Nsp5/Mpro/3CL Protein, N-His.pdf