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Catalog No.
KAK13902
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Tirzepatide has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-Tirzepatide (LY3298176) Human IgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP conjugated probe is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Tirzepatide (LY3298176) Human IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Tirzepatide (LY3298176) Human IgG concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
3.13 - 200 ng/mL
Sensitivity
2.35 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
83.1 |
22.1 |
5.8 |
93.9 |
22.2 |
5.7 |
|
Standard deviation |
5.0 |
1.6 |
0.3 |
4.5 |
1.2 |
0.5 |
|
CV (%) |
6.1 |
7.1 |
5.8 |
4.7 |
5.5 |
8.9 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.
Alternative Names
Tirzepatide, LY3298176, CAS: 2023788-19-2
For research use only. Not for human or drug use.
